Method for propagation culture and solid fermentation of white-rot fungi

A technology of expanded culture and solid fermentation, applied in microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of weak pertinence, complicated operation, high cost, and achieve strong pertinence and low fermentation cost. , the effect of convenient operation

Inactive Publication Date: 2009-12-02
CHONGQING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to overcome the deficiencies of the above-mentioned prior art, and propose a method for solid fermentation of white rot fungus enlarged culture, solve the problems of low efficiency, complicated operation, high cost, and not strong pertinence

Method used

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  • Method for propagation culture and solid fermentation of white-rot fungi
  • Method for propagation culture and solid fermentation of white-rot fungi
  • Method for propagation culture and solid fermentation of white-rot fungi

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Embodiment Construction

[0020] Below in conjunction with embodiment, technical scheme of the present invention is further described as follows:

[0021] Take out the white rot fungus strains (Phanerochaete chrysosporium, Trametes vermilion (purchased from Institute of Microbiology, Chinese Academy of Sciences), Pleurotus ostreatus (purchased from Chongqing Institute of Agricultural Sciences)) preserved at 4°C from the refrigerator, and place at 35°C In the incubator for 25 minutes, the PDA medium was sterilized at 0.5 MPa at 121°C for 30 minutes, divided into petri dishes and cooled, then inoculated, then wrapped with a safety film and placed in a 32°C biochemical incubator for 3 to 5 days. 250ml triangular bottle liquid 90ml (each liter contains bean sprouts juice 200mL, glucose 10.0g, potassium dihydrogen phosphate (KH 2 PO 4 )3.0g, VB 1 0.001g, Tween 202.0g, magnesium sulfate (MgSO 4 ·7H 2 O) 1.5g, ferrous sulfate (FeSO 4 ·7H 2O) 0.2g, manganese sulfate (MnSO 4 ·H 2 O) 0.2g, copper sulfate...

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Abstract

The invention relates to a method for propagation culture and solid fermentation of white-rot fungi, which belongs to a method for fungal solid fermentation and comprises the following steps: activation of strains; liquid propagation culture; fermentation tank liquid propagation culture; and solid culture and domestication in a canned bottle, and fermentation in a fermentation tank, wherein the inoculum sizes of the stage of the solid culture and domestication in the canned bottle and the stage of the fermentation in the fermentation tank are 10 percent and 5 percent respectively. The method has the following advantages: the method has simple technical process so as to be directly applied to large-scale fermentation tanks in factories, has strong the pertinence so as to be applied to sewage treatment and mixed white-rot fungi fermentation in the paper industry, and has high use value, high fermentation efficiency, low fermentation cost and the like.

Description

technical field [0001] The invention belongs to a method for solid fermentation of fungi, in particular to a method for expanded cultivation of white rot fungus solid fermentation. Background technique [0002] The unique and effective degradation ability of white rot fungi makes it a powerful new force in the field of environmental protection. It transforms the active potential of this kind of filamentous fungi with spectrum attack on xenobiotics into media of different states in the environment. Realistic control of pollutants in the environment is the common pursuit goal and unremitting direction of efforts of scientists and related industries in various countries. Although compared with the mature bacterial system, the application of white rot fungi in environmental protection is still in the stage of exploration and establishment; although due to the particularity of the physiological metabolism of this type of bacteria and the almost harsh requirements for environmenta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
Inventor 段传人贾秋云朱丽平唐菊胡江
Owner CHONGQING UNIV
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