The invention discloses a probiotic drop for improving allergic constitution and a preparation method of the probiotic drop, and belongs to the technical field of medical biology. The probiotic drop comprises the following raw materials: lactobacillus casei, lactobacillus paracasei, lactobacillus rhamnosus, lactobacillus reuteri, lactobacillus acidophilus, bifidobacterium longum, lactobacillus plantarum, fermented lactic acid bacteria, galactooligosaccharide, xylooligosaccharide, anhydrous glucose, protein hydrolysate, polyhydric alcohols, natural vitamin E, unsaturated fatty acid, vegetable oil and phospholipid oil. The preparation method of the drop is simple, the process is easy to control, and the drop is stable in performance, not solidified at low temperature, uniform in component, good in oxidation resistance and more stable in storage.