46results about How to "Effective colonization" patented technology

The invention discloses a micro-ecological flora of crop root system probiotics and an application thereof. The micro-ecological flora comprises a leading flora, a balance flora, a basic flora, a secondary-function flora and a special-function flora, wherein the leading flora comprises 25-35 parts of nitrogen-fixing bacteria by mass; the balance flora comprises 17-23 parts of phosphate solubilizing bacteria and 17-23 parts of potassium solubilizing bacteria by mass; the basic flora comprises 2-7 parts of yeast, 2-7 parts of rhizobia and 2-7 parts of photosynthetic bacteria by mass; the secondary-function flora comprises 2-7 parts of bacillus and 2-7 parts of actinomycetes by mass; the special-function flora comprises 2-4 parts of white rot fungi and 1-3 parts of pseudomonas. In the micro-ecological flora disclosed by the invention, the strain stability is good, functional microorganisms can be effectively planted in soil, and the functions of improving the crop yield and quality, improving the soil, detoxifying heavy metals and resisting diseases and pests can be realized.

The invention discloses a microbial preparation for preventing and controlling plant root knot nematodes and the application thereof. The preparation is mixed from Paecilomyces lilacinus, Bacillus subtilis, shell powder, shrimp shell powder, weathered coal humic acid and peat. The shell powder and shrimp shell powder are added to induce paecilomyces lilacinus to produce chitinase. The preparation can be stored at room temperature, and after storage for 90 days at 25 DEG C, the survival rate of paecilomyces lilacinus is more than 80%. The tomato pot experiment and cucumber field test prove that the preparation has remarkable effect on the control of root knot nematodes; at the same time, soil colonization test verify the Paecilomyces lilacinus in the preparation has good colonization condition in soil.

The present invention provides a probiotics fermentation milk. According to the present invention, match of flora and nutrients, and reasonable material use amount ratio are adopted to effectively improve field planting effects of probiotics in intestinal tract, probiotics activity is provided, and health effects of good taste, immunity improving and cholesterol lowering are provided.

The invention discloses probiotics compound cranberry female reproduction health nutrition supplementary. The nutrition supplementary is prepared from the following raw materials of active probiotics micro capsules, cranberry fruit powder, collagen, fructo-oligose, herba lophatheri, soy isoflavone, grape fruit powder and vitamin C, wherein the probiotics micro capsules are 9-11 billion colony forming unit (CFU), the cranberry fruit powder is 3-7 weight parts, the collagen is 1-10 weight parts, the fructo-oligose is 8-15 weight parts, the herba lophtheri is 1-10 weight parts, the soy isoflavone is 9-25 weight parts, the grape fruit powder is 1-20 weight parts, and the vitamin C is 0.01-0.05 weight part. When the cranberry fruit powder and the active probiotics micro capsules are combined and used according to a reasonable proportion, flora balance is effectively maintained, and an antibacterial effect is improved by one time. Compared with the prior art, the probiotics compound cranberry female reproduction health nutrition supplementary has the advantages of effectively improving female reproduction health and being convenient to take ,little in side effect and the like.

The invention provides a trichoderma harzianum-contanining functional vegetable seedling biological matrix and a preparation method thereof. The functional vegetable seedling biological matrix is formed by adding a functional bacterial strain, namely the trichoderma harzianum SQR-T037, into a common seedling matrix. As for seedlings such as cucumbers, eggplants and watermelons, the specific fungus trichoderma harzianum SQR-T037 is selected, is kept alive and is added into the matrix to obtain an active biological matrix product, and by using the product, the plant growth promoting effect on seedling stages of seedlings such as the cucumbers, eggplants and watermelons is good, and high-quality seedlings of which the rhizospheres include a large amount of microorganisms with an activity function can be cultivated; meanwhile, the beneficial microorganisms can be in large-scale colonization at the rhizospheres of the seedlings, so that the growth of the cultivated seedlings in the later cultivation process can be promoted, the yield of the crops planted in a big field is improved, and the effect of preventing and controlling a cucumber soil-borne disease of the cultivated seedlings is achieved.

The invention belongs to the field of health food and specifically relates to fungus and polysaccharide and probiotic composite function food. The functional food is composed of the following raw materials (by weight): 30-80 parts of a ganoderma extract, 20-40 parts of a tremella extract, 10-15 parts of lentinan, 2-10 parts of fructo-oligosaccharide, 2-5 parts of wheat fiber, 2-5 parts of taurine, 0.01-0.03 part of tea polyphenol, 10-50 parts of malt extract and 20-30 parts of lactobacillus plantarum powder. By the extraction method of polysaccharide, rate of polysaccharide extraction is boosted, the product has a good effect of raising immunity, and the product has good efficacy of degrading cholesterol.

The invention discloses a bacillus megatherium QBJP-F6. The bacillus megatherium QBJP-F6 is preserved in the Common Microorganism Center of the China Committee for Culture Collection of Microorganisms, and the preservation number is CGMCC NO.12888. The invention further discloses an application of the bacillus megatherium QBJP-F6 in preparation of a functional vegetable seedling biological matrix. The QBJP-F6 can effectively colonize the rhizosphere of a seedling, and a bacterial strain has a high growth promoting function and can enhance growth in seedling later-period potting and field planting. The functional vegetable seedling biological matrix has good growth promoting effect on vegetables at the seedling stage and can significantly promote growth of various vegetables at the seedling stage, high-quality seedlings of which the rhizospheres are provided with a large number of active function microorganisms can be obtained, and growth of the seedlings in the later-period cultivation process can be significantly promoted.

The present invention discloses a casein and bone protocalcium compound probiotic preparation which is made from the following raw materials: galactooligosaccharides, casein phosphopeptides, bone protocalcium and probiotics. The use amount of the casein phosphopeptides ranges from 15%-35%; the use amount of the bone protocalcium ranges from 2%-6%; the use amount of the probiotics ranges from 0.1%-1%; and the balance is galactooligosaccharides. The activated probiotics are prepared by using a double-layer embedding microencapsulated technology, the probiotics are bifidobacterium lactis and lactobacillus rhamnosus, and the bifidobacterium lactis and lactobacillus rhamnosus are at a ratio of 1 : 1, and preferably the product can achieve the best effect using a 37 DEG C or less warm water or milk for administration. Compared with the prior art, the compound probiotic preparation can effectively promote calcium absorption, and is low in toxic and side effects, wide in application range, long in activity, is resistant to gastric acid, etc.

The invention discloses a water culture method of dwarf bamboo suitable for laboratory scientific research and a culture device thereof. The culture device is firstly manufactured and comprises waterculture barrels, a fixing device and a ventilating device, wherein diluted working nutrient solution is added into each water culture barrel, dwarf bamboo plants required by the experiment are selected, the root system original soil is removed, the root system is washed by using clear water, the over ground part of the cleaned dwarf bamboo is fixed above a fixing device, the underground part is placed below the fixing device, the fixing device and the bamboo seedlings are placed into the water culture barrel together, and the bamboo seedlings are treated at the stage when the original soil culture root system is replaced and the new aquatic root system is induced, the bamboo seedlings are treated at the growth stage of the aquatic root system. The method and the device can achieve the water culture of the dwarf bamboo, and the whole transplanting process from the unearthed bamboo seedlings to the planting in the water culture device is beneficial to the material taking test of each organ of the dwarf bamboo, thereby expanding the wider scientific research application in a laboratory, the rhizomes can effectively absorb nutrients required by the plants.

The invention discloses a functional vegetable seedling biological matrix containing bacillus subtilis G10 and a preparation method thereof. The bacillus subtilis G10 with the preservation number of CGMCC NO.9921 is preserved in the China General Microbiological Culture Collection Center on November 3, 2014. The functional vegetable seedling biological matrix is obtained by adding the bacillus subtilis G10 into a conventional seedling matrix. The functional vegetable seedling biological matrix has a good growth promotion effect for vegetable and fruit seedling stage to obtain high-quality seedlings, and can be used for remarkably reinforcing growth of seedlings in field planting in the late stage since beneficial microorganisms can be colonized massively in seedling rhizosphere.

The invention discloses a probiotics and Bombyx mori compounded diabetic patient nutritional supplement.The probiotics and Bombyx mori compounded diabetic patient nutritional supplement is prepared from, by weight, 90-110 CFU of activated probiotics micro-capsules, 3-8 parts of aminobutyric acid, 9-12 parts of xylooligosaccharide, 1-5 parts of zinc-rich yeast, 2-6 parts of mulberry polysaccharide, 1-3 parts of silkworm pupa protein peptide and 10-24 parts of dietary fibers.Multiple activated probiotics are matched with short peptides, amino acids, the water-soluble dietary fibers and the like reasonably, growth and reproduction of intra-intestinal probiotics are promoted by the activated probiotics, and silkworm pupa polypeptides cooperate with biologically active substances such as active polysaccharides to decrease blood sugar.Compared with the prior art, the probiotics and Bombyx mori compounded diabetic patient nutritional supplement has the advantages of capability of decreasing blood sugar effectively, convenience in eating, little toxic or side effect and the like.

The invention belongs to the field of health food and specifically relates to agaric polysaccharide functional food. The functional food is composed of the following raw materials (by weight): 20-50 parts of auricularia auricula polysaccharide, 20-50 parts of tremella polysaccharide, 10-20 parts of ganoderma lucidum polysaccharide, 1-10 parts of taurine, 3-5 parts of L-arginine, 2-10 parts of fructo-oligosaccharide, 0.01-0.02 part of tea polyphenol, 10-80 parts of malt extract and 10-20 parts of lactobacillus plantarum powder. By the extraction method of polysaccharide, rate of polysaccharide extraction is boosted, the product has a good effect of enhancing immunity, and the product has good efficiency of degrading cholesterol.

The invention discloses a probiotic compound oligopeptide nutritional supplement capable of lowering uric acid. The probiotic compound oligopeptide nutritional supplement is prepared from the following raw materials in parts by weight: 2-7 parts of ocean fish oligopeptide powder, 6-14 parts of collagen, 12-24 parts of xylooligosaccharide, 4-9 parts of common lophatherum herb, 20-40 parts of sorbitol, 6-10 parts of citric acid and 0.01-0.05 part of vitamin C, as well as 9-11 billion CFU of active probiotic micro-capsules. Compared with the prior art, the product has the advantages of being capable of effectively lowering uric acid, having good taste, having anti-ageing effect and small toxic and side effect, and being convenient to eat.

The invention discloses probiotics fermented milk and a preparation technology thereof. The preparation technology comprises the following steps of pretreating, centrifuging and separating, fermenting, material mixing, filling and storing. The preparation technology has the advantages that multiple bacterial strains are inoculated at one time in the fermenting process, and the temperature in different fermenting phases is controlled to control the growth rates of different bacterial strains, so that the different bacterial stains can reach the pilot function; the survival rate of probiotics in the fermenting process is improved; the production technology is simplified, and the probiotics fermented milk with high nutritional value, more probiotics and long quality warranty period is obtained.

The invention discloses application of drug combination of bacteroides fragilis zwitterionic capsular polysaccharide and a PD-1 antibody and / or a PD-L1 antibody in prevention and treatment of respiratory system tumors. A large number of experiments prove that the bacteroides fragilis zwitter-ion capsular polysaccharide, especially the zwitter-ion capsular polysaccharide extracted from bacteroides fragilis ZY-312 with the preservation number of CGMCC No.10685, and the PD-1 antibody / PD-L1 antibody are combined for use, so that the immune cell proportion and the immune factor level can be adjusted, the anti-tumor immune response of a body is enhanced, and the respiratory system tumors are effectively prevented and treated.

The invention discloses application of bacteroides fragilis and / or zwitterionic capsular polysaccharide thereof in preparation of a medicine for preventing and treating cancers of a urogenital system. The bacteroides fragilis, especially the bacteroides fragilis ZY-312 with the preservation number of CGMCC No.10685 and the zwitter-ion capsular polysaccharide A of the bacteroides fragilis ZY-312 can regulate the levels of T cells and immune factors, enhance body immunity and effectively prevent and treat kidney cancer, bladder cancer, prostate cancer, bladder urinary tract epithelial cancer, ovarian cancer, breast cancer, cervical cancer and other urogenital system tumors.

The invention provides a culture medium for promoting synergistic proliferation of lactobacillus and bifidobacterium and application of the culture medium. The culture medium is prepared from the following components in percentage by mass: 2 to 5 percent of prebiotic composition, 0.5 to 5 percent of peptone, 0.5 to 2 percent of beef extract, 0.1 to 2 percent of yeast extract, 0.05 to 1 percent of diammonium hydrogen citrate, 0.05 to 1 percent of K2HPO4, 0.01 to 1 percent of MgSO4, 0.01 to 1 percent of MnSO4, 0.05 to 1 percent of tween-80 and 0.05 to 1 percent of L-cysteine hydrochloride. The prebiotic composition is prepared from stachyose, mannose oligomer and xylooligosaccharide according to the mass ratio of (1 to 2): (1 to 2): (0.1 to 1). The composite prebiotic culture medium can obviously improve the proliferation and acid production capacity of lactobacillus and bifidobacterium, and the activity and functions of common beneficial bacteria in the intestinal tract are ensured through high-density culture, so that the common beneficial bacteria can be effectively colonized in the intestinal tract.

The invention discloses an application of bacteroides fragilis combined with an immune checkpoint inhibitor in treatment of skin tumors, in particular to a bacteroides fragilis ZY-312 with the preservation number of CGMCC No.10685, and the bacteroides fragilis ZY-312 is combined with a PD-1 inhibitor for use, so that the comprehensive curative effect of treating the skin tumors can be remarkably improved; the bacteroides fragilis can effectively prevent and treat generation, development, relapse and metastasis of melanoma by reducing the levels of proinflammatory factors IL-1, IL-6, IL-8 and VEGF, increasing migration of CD8 + effector T cells, reducing recruitment of Treg cells, reducing the weight of in-situ tumors and increasing the cancer inhibition rate, the harm of radiotherapy and chemotherapy to organisms is relieved, and the living quality of patients is improved.

The invention discloses an application of Bacteroides fragilis and / or zwitterionic capsular polysaccharide thereof in preparation of a medicine for treating respiratory system tumors, the Bacteroides fragilis, especially the Bacteroides fragilis ZY-312 with the preservation number of CGMCC No.10685 and the zwitterionic capsular polysaccharide thereof, can be used for preparing medicines for treating respiratory system tumors by increasing the levels of anti-tumor factors IL-12 and IFN-gamma, and can be used for preparing medicines for treating respiratory system tumors. The traditional Chinese medicine composition can be used for inhibiting expression of a tumor promoting factor IL-1beta, promoting up-regulation of the proportion of infiltrated CD8 + CD45 + T cells in tumors, regulating the microenvironment of tumor tissues, reducing the weight of in-situ tumors and inhibiting the number of tumor metastases, can be used for effectively preventing and treating respiratory system tumors, can be independently used for cancer treatment, and also can be used for preventing and treating tumors. The traditional Chinese medicine composition can also be combined with other treatment means such as microorganisms, operations, radiotherapy and chemotherapy, so that the comprehensive curative effect is remarkably improved, the harm of radiotherapy and chemotherapy to organisms is relieved, occurrence, development, relapse and metastasis of respiratory system tumors are effectively prevented, and the living quality of patients is improved.

The invention discloses bacteroides fragilis, in particular to combined application of bacteroides fragilis ZY-312 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.10685 and a PD-1 antibody and / or a PD-L1 antibody, and the bacteroides fragilis ZY-312 and the PD-1 antibody and / or the PD-L1 antibody are used for correcting the proportion of immune cells of a body, regulating the level of cancer-related cytokines of the body, improving the anti-tumor immune response of the body and effectively preventing and treating tumors of a respiratory system

The invention relates to the technical field of functional microorganism screening and application, in particular to novel bacillus subtilis and application thereof in aquaculture. The bacillus subtilis is screened from intestinal tracts of litopenaeus vannamei, the preservation number is CCTCC NO: M 2020091, pathogenic bacteria can be significantly inhibited, the immunity and disease resistance of aquatic animals are improved, protease and amylase are produced to improve feed digestion and utilization, and the growth of the aquatic animals is promoted, and the bacillus subtilis can be widelyapplied to the field of aquatic feeds.

The invention discloses application of combined medication of bacteroides fragilis zwitterionic capsular polysaccharide and an immune checkpoint inhibitor in prevention and treatment of tumors of a genitourinary system. A large number of experiments prove that when the capsular polysaccharide A of the bacteroides fragilis, especially the bacteroides fragilis ZY-312 with the preservation number of CGMCC No.10685, and an immune checkpoint inhibitor are combined for medication, the anti-tumor immune response of a body can be enhanced by regulating and controlling the levels of T cells and anti-tumor immune factors, and then the aim of preventing and treating the tumors of the genitourinary system is achieved.

Belonging to the technical field of food processing, the invention discloses a preparation method of composite probiotic powder. The composite probiotic powder comprises freeze-dried fungus powder, prebiotics and sugar alcohols; the freeze-dried fungus powder is screened from healthy intestinal tracts of Chinese human bodies and traditional fermented foods, and is more suitable for the intestinaltracts of Chinese people. And the composite probiotic powder has the efficacy of relieving constipation and atopic dermatitis pruritus, regulating intestinal flora, relieving hyperlipidemia, relievingheavy metal toxicity, resisting oxidation, relieving inflammation, regulating intestinal flora, resisting oxidation, scavenging free radicals, reducing blood pressure, relieving type II diabetes mellitus and the like. According to the preparation method disclosed by the invention, the probiotic liquid is firstly concentrated, then freeze-drying is carried out to remove moisture, and then the product is uniformly mixed with granulated four prebiotics and three sugar alcohols according to a certain proportion, so that the flavor and taste of the product are greatly improved, and the product canalso be taken by diabetics orally.

The invention discloses an application of a combination of bacteroides fragilis and an immune checkpoint inhibitor in treatment of digestive system tumors, and particularly relates to a combination of bacteroides fragilis ZY-312 with the preservation number of CGMCC No.10685, inactivated bacteria of the bacteroides fragilis ZY-312 and the immune checkpoint inhibitor including a PD-1 antibody or a PD-L1 antibody, and the bacteroides fragilis ZY-312, the inactivated bacteria of the bacteroides fragilis ZY-312 and the immune checkpoint inhibitor including the PD-1 antibody or the PD-L1 antibody can be combined in vivo by increasing the proportion of CD4 + T cells and CD8 + T cells. By down-regulating the level of cell factors IL-10 in tumors or up-regulating the level of INF-gamma, the growth of transplanted tumors in mice can be effectively inhibited. The traditional Chinese medicine composition can also be combined with treatment means such as operation, radiotherapy and chemotherapy, so that the comprehensive curative effect is remarkably improved, the harm of radiotherapy and chemotherapy to the body is relieved, occurrence, development, relapse and metastasis of tumors are effectively prevented, and the living quality of patients is improved.

The invention discloses application of drug combination of bacteroides fragilis and a PD-1 antibody and / or a PD-L1 antibody in prevention and treatment of tumors of a genitourinary system. A large number of experiments prove that the bacteroides fragilis, especially the bacteroides fragilis ZY-312 with the preservation number of CGMCC No.10685, can be used for effectively preventing and treating the tumors of the genitourinary system by regulating immune factors, improving the state of immune cells and enhancing the anti-tumor immune response of a body.

The invention discloses a recombinant bacillus subtilis and an application thereof. According to the recombinant bacillus subtilis, a gene for coding antiviral polypeptide and a gene for coding spore coat protein in bacillus subtilis are fused, exogenous fusion expression is carried out in the bacillus subtilis, and the recombinant bacillus subtilis capable of displaying the antiviral polypeptide on the spore surface of the bacillus subtilis is obtained. The antiviral polypeptide is derived from feline coronavirus, and the amino acid sequence of the antiviral polypeptide is as shown in SEQ ID No.2. The spores generated by the recombinant bacillus subtilis immunize mice by means of intragastric administration and nasal drip, can activate strong mucosal immune response in intestinal mucosa and specific and systemic immune response in mice, can resist adverse environment of gastrointestinal tracts, and are effectively colonized, so the spore of the recombinant bacillus subtilis is a potential excellent carrier.

The invention discloses application of bacteroides fragilis and / or zwitterionic capsular polysaccharide thereof in preparation of drugs for preventing and treating digestive system tumors. A large number of experiments prove that the bacteroides fragilis ZY-312 with the preservation number of CGMCC No.10685 and the zwitterionic capsular polysaccharide thereof can relieve inflammatory response, protect normal tissues, improve anti-tumor immune response of organisms and effectively prevent and treat digestive system tumors.

The invention discloses paenibacillus QBJP-F4, which is preserved in China General Microbiological Culture Collection Center, with preservation number of CGMCC NO.12887. The invention also discloses an application of the paenibacillus QBJP-F4 in preparing a functional vegetable biological breeding medium. The QBJP-F4 is capable of achieving effective colonization in seedling rhizosphere; and the strain (the QBJP-F4) is relatively strong in growth promoting function and is capable of enhancing the grow the of bred seedlings in later potting and field planting. The functional vegetable biological breeding medium has a good growth promoting effect in a vegetable seedling stage, and the breeding medium is capable of obviously promoting the growth of a plurality of vegetables in the seedling stage, obtaining high-quality seedlings which contain a great amount of active functional microorganisms in rhizosphere and remarkably promoting the growth of the bred seedlings in a later planting process.

The invention discloses a probiotic drop for improving allergic constitution and a preparation method of the probiotic drop, and belongs to the technical field of medical biology. The probiotic drop comprises the following raw materials: lactobacillus casei, lactobacillus paracasei, lactobacillus rhamnosus, lactobacillus reuteri, lactobacillus acidophilus, bifidobacterium longum, lactobacillus plantarum, fermented lactic acid bacteria, galactooligosaccharide, xylooligosaccharide, anhydrous glucose, protein hydrolysate, polyhydric alcohols, natural vitamin E, unsaturated fatty acid, vegetable oil and phospholipid oil. The preparation method of the drop is simple, the process is easy to control, and the drop is stable in performance, not solidified at low temperature, uniform in component, good in oxidation resistance and more stable in storage.

The invention relates to the technical field of functional microorganism screening and application, and particularly provides a micro-ecological composite additive containing bacillus subtilis DN02 and application of the micro-ecological composite additive in aquaculture. The bacillus subtilis DN02 is screened from intestinal tracts of litopenaeus vannamei, the preservation number is CCTCC NO: M2020091, pathogenic bacteria can be significantly inhibit, the immunity and disease resistance of aquatic animals are improved, protease and amylase are produced to improve feed digestion and utilization, the growth of the aquatic animals is prompted, and the bacillus subtilis DN02 can be widely applied to the field of aquatic feeds.