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Paenibacillus QBJP-F4 and application thereof

A QBJP-F4, Paenibacillus technology, applied in the field of agricultural microorganisms, can solve the problems of not forming large-scale promotion products, insufficient systematic and in-depth research, etc., and achieve the effect of promoting survival ability, good growth promoting effect, and promoting growth.

Active Publication Date: 2017-03-22
南京秦邦吉品农业开发有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, some domestic experts are trying to work in this area, mainly focusing on using AM mycorrhiza to inoculate existing substrates for seedling cultivation. Although some results have been achieved, the research is not systematic and in-depth, nor has it formed a large-scale promotion and has been recognized by the majority of farmers. products, so the research on this patent becomes particularly important

Method used

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  • Paenibacillus QBJP-F4 and application thereof
  • Paenibacillus QBJP-F4 and application thereof
  • Paenibacillus QBJP-F4 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1, isolation and identification of bacterial strain

[0033] Take 5g of soil in a triangular flask filled with 45ml of sterile water, and shake it in a shaker at 30°C and 170r / min for 20min to make a soil suspension. After 20min in a water bath at 80°C, dilute to 10 -1 、10-2 、10 -3 、10 -4 、10 -5 After waiting for different gradient dilutions, smear the plate, incubate in the dark for 1d to 2d in the incubator, and then pick a single colony, after purification on the plate, select 12 strains in total, and measure the ability of each strain to produce the auxin indole acetic acid (IAA) (Method reference: Glickmann E, DessauxY. A critical examination of the specificity of the salkowski reagent forindolic compounds produced by phytopathogenic bacteria. [J]. Applied and environmental microbiology, 1995, 612). Using the same method, a strain QBJP-F4 with excellent growth-promoting ability was obtained as a result of primary screening and re-screening, which was ...

Embodiment 2

[0036] Embodiment 2, the production of functional bacteria fermented liquid

[0037] The strain QBJP-F4 (CGMCC NO.12887) was inoculated into PDA culture medium for liquid fermentation production. The conditions of fermentation production were: pH 7.0, fermentation temperature 30°C, stirring speed 170rpm / min, spores formed in the middle and late stages of fermentation, The fermentation time is 48 hours, so that the amount of bacteria or spores in the fermentation broth is ≥1×10 10 a / ml;

[0038] The preparation method of the PDA culture medium used is as follows, taking the preparation of 1L medium as an example: 200g potatoes are peeled, cut into small pieces, put them in water and boil them, boil them for 30min and then filter them, add 20g ordinary sucrose to the filtrate and make the volume to 1000ml , natural pH, sterilized at 121°C for 20min.

Embodiment 3

[0039] Example 3, research and development of functional biological matrix

[0040] The effect of biological matrix seedling cultivation with strain QBJP-F4 as functional bacteria

[0041] Research and development of the substrate: the fermentation broth of the bacterial strain QBJP-F4 prepared in Example 2 was inoculated (according to 5v / w% of the dry weight of the substrate, the same below) into the common seedling cultivation substrate, and mixed evenly. The functional strain QBJP-F4 in the matrix was counted by selective medium, and the formula of selective medium was: peptone 10.0g, yeast powder 5.0g, NaCl 10.0g, agar 2.0%, deionized water 1000mL, pH 7.2-7.4, 121 ℃ autoclave for 20min. 1% polymyxin 2mL, 1% cycloheximide 4mL (the antibiotics are added after the medium is cooled before being poured). The number of colonies of functional bacteria QBJP-F4 is calculated by dry weight per gram of substrate. After counting, the number of functional bacteria in the biological ...

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Abstract

The invention discloses paenibacillus QBJP-F4, which is preserved in China General Microbiological Culture Collection Center, with preservation number of CGMCC NO.12887. The invention also discloses an application of the paenibacillus QBJP-F4 in preparing a functional vegetable biological breeding medium. The QBJP-F4 is capable of achieving effective colonization in seedling rhizosphere; and the strain (the QBJP-F4) is relatively strong in growth promoting function and is capable of enhancing the grow the of bred seedlings in later potting and field planting. The functional vegetable biological breeding medium has a good growth promoting effect in a vegetable seedling stage, and the breeding medium is capable of obviously promoting the growth of a plurality of vegetables in the seedling stage, obtaining high-quality seedlings which contain a great amount of active functional microorganisms in rhizosphere and remarkably promoting the growth of the bred seedlings in a later planting process.

Description

technical field [0001] The invention relates to the field of agricultural microorganisms, in particular to a Paenibacillus QBJP-F4 and its application in preparing functional vegetable seedling-raising biological substrates. Background technique [0002] With the development of my country's modern agriculture, more and more people pay more and more attention to industrial vegetable seedling cultivation, and plug seedling cultivation is the main measure of current industrial seedling cultivation. According to incomplete statistics, the area of ​​crops that need to be raised in my country is very large, among which the planting area of ​​rice exceeds 300 million mu / year, and the area of ​​economic crops exceeds 500 million mu / year. At present, in China, materials rich in organic matter (such as peat, decomposed plant residues, etc.) are mainly used, and an appropriate proportion of light inorganic materials, such as vermiculite and expanded perlite, are used to make seedling s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01G9/10C12R1/01
CPCA01G24/00C12N1/205C12R2001/01
Inventor 李荣巩子毓秦俊俞萍
Owner 南京秦邦吉品农业开发有限公司
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