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Application of bacteroides fragilis combined with immune checkpoint inhibitor in treatment of skin tumors

A technology of Bacteroides fragilis and inhibitors, applied in the field of probiotics, to achieve the effects of reducing weight, improving quality of life, and improving comprehensive curative effect

Active Publication Date: 2022-04-15
GUANGZHOU ZHIYI PHARMA INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no literature on the use of Bacteroides fragilis in combination with immune checkpoint inhibitors for the treatment of cutaneous melanoma

Method used

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  • Application of bacteroides fragilis combined with immune checkpoint inhibitor in treatment of skin tumors
  • Application of bacteroides fragilis combined with immune checkpoint inhibitor in treatment of skin tumors
  • Application of bacteroides fragilis combined with immune checkpoint inhibitor in treatment of skin tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1: the fermentation culture of Bacteroides fragilis

[0054] Streak inoculation of Bacteroides fragilis ZY-312 strain on blood plate, anaerobic culture for 48h. Observe the colony morphological characteristics, staining characteristics, size, club shape and distribution, etc.

[0055] Colony characteristics: Bacteroides fragilis ZY-312, after being cultured on a blood plate for 48 hours, is slightly convex, translucent, white, smooth, non-hemolytic, and the diameter of the colony is between 1-3mm. See figure 1 .

[0056] Morphology under the microscope: Bacteroides fragilis ZY-312 was examined by Gram staining. It is a Gram-negative bacterium, showing a typical rod shape, with blunt rounded ends and dense staining.

[0057] Select a single colony and inoculate it in a plant-derived peptone liquid medium for fermentation and culture for 8 hours (at a temperature of 37°C). The obtained bacterial liquid is centrifuged and precipitated at a speed of 3000r / min f...

Embodiment 2

[0058] Embodiment 2: Preparation of Bacteroides fragilis live bacteria liquid

[0059] 1) Streak-inoculate the strain on a blood plate and incubate anaerobically at 37°C for 48 hours.

[0060] Colony characteristics: Bacteroides fragilis ZY-312, after being cultured on a blood plate for 48 hours, is slightly convex, translucent, white, smooth, non-hemolytic, and the diameter of the colony is 1-3mm.

[0061] 2) Enrichment: Select a single colony from step 1) and inoculate in TSB (tryptone soybean broth, containing 5% fetal bovine serum) for enrichment culture, and the obtained bacterial solution is preserved for future use.

[0062] 3) Viable Bacteroides fragilis solution: the bacterial solution prepared in step 1) was used to measure the number of bacteria with a McFarland turbidimetric tube, and diluted to 10 with normal saline. 7 CFU / ml and 10 9 CFU / ml, save for future use. Used in the following examples.

Embodiment 3

[0063] Embodiment 3: Preparation of the inactivated bacteria powder of Bacteroides fragilis

[0064] (1) Take the Bacteroides fragilis fermented liquid obtained in Example 1 and carry out centrifugation to the fermented liquid, collect the wet thalline, add physiological saline according to the thalline: normal saline=1:(10~30) (m:v) ratio Resuspend and wash the bacteria sludge, and centrifuge again to collect the washed bacteria;

[0065] (2) Add 5% maltodextrin+0.9% sodium chloride to the thallus obtained in step (1) as an excipient mixed, according to the thallus: excipient=1:(5~15)(m:m) Add in proportion, stir and disperse, heat inactivate at (70-100)±5°C for (20-40)±5 minutes to obtain inactivated bacteria solution;

[0066] (3) Centrifuge the inactivated bacteria liquid obtained in step (2) to collect the inactivated bacteria sludge.

[0067] (4) Add excipients to the inactivated bacteria slime collected in step (3) to make the total weight consistent with the weight o...

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Abstract

The invention discloses an application of bacteroides fragilis combined with an immune checkpoint inhibitor in treatment of skin tumors, in particular to a bacteroides fragilis ZY-312 with the preservation number of CGMCC No.10685, and the bacteroides fragilis ZY-312 is combined with a PD-1 inhibitor for use, so that the comprehensive curative effect of treating the skin tumors can be remarkably improved; the bacteroides fragilis can effectively prevent and treat generation, development, relapse and metastasis of melanoma by reducing the levels of proinflammatory factors IL-1, IL-6, IL-8 and VEGF, increasing migration of CD8 + effector T cells, reducing recruitment of Treg cells, reducing the weight of in-situ tumors and increasing the cancer inhibition rate, the harm of radiotherapy and chemotherapy to organisms is relieved, and the living quality of patients is improved.

Description

technical field [0001] The invention relates to the technical field of probiotics, in particular to the application of Bacteroides fragilis combined with immune checkpoint inhibitors in the treatment of skin tumors. Background technique [0002] Melanoma is a kind of highly malignant tumor originating from melanocytes, which can occur in different parts or tissues such as skin, mucous membrane (digestive tract, respiratory tract, genitourinary tract, etc.), uvea, pia mater, etc. It is a malignant tumor with high mortality, high metastasis rate and difficult treatment. There are nearly 20,000 new cases of melanoma in China every year, and the incidence rate is increasing year by year. There are as many as 3,800 deaths each year, accounting for 1 / 3 of all Asian patients, ranking first in Asia. Among them, skin melanoma has a high degree of malignancy, rapid growth, and mostly poor prognosis. Therefore, early diagnosis and timely and correct treatment are very important. Trea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/741A61K45/06A61K39/395A61P35/00
CPCY02A50/30C07K14/195A61K39/395A61P35/00A61K45/06A61K35/741
Inventor 王晔郑丽君黄烁雅吴家棋
Owner GUANGZHOU ZHIYI PHARMA INC
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