Culture medium for promoting synergistic proliferation of lactobacillus and bifidobacterium and application of culture medium

Abstract

The invention provides a culture medium for promoting synergistic proliferation of lactobacillus and bifidobacterium and application of the culture medium. The culture medium is prepared from the following components in percentage by mass: 2 to 5 percent of prebiotic composition, 0.5 to 5 percent of peptone, 0.5 to 2 percent of beef extract, 0.1 to 2 percent of yeast extract, 0.05 to 1 percent of diammonium hydrogen citrate, 0.05 to 1 percent of K2HPO4, 0.01 to 1 percent of MgSO4, 0.01 to 1 percent of MnSO4, 0.05 to 1 percent of tween-80 and 0.05 to 1 percent of L-cysteine hydrochloride. The prebiotic composition is prepared from stachyose, mannose oligomer and xylooligosaccharide according to the mass ratio of (1 to 2): (1 to 2): (0.1 to 1). The composite prebiotic culture medium can obviously improve the proliferation and acid production capacity of lactobacillus and bifidobacterium, and the activity and functions of common beneficial bacteria in the intestinal tract are ensured through high-density culture, so that the common beneficial bacteria can be effectively colonized in the intestinal tract.

Description

Technical field [0001] The invention belongs to the technical field of microorganisms, and specifically relates to a culture medium that promotes the coordinated proliferation of Lactobacillus and Bifidobacterium and its application. Background technique [0002] Prebiotics refer to carbohydrates that cannot be digested and utilized by the human body but can selectively promote the proliferation and metabolism of intestinal probiotics. Prebiotics are essentially oligosaccharides and bifid factors, such as fructooligosaccharides, galacto-oligosaccharides, and mannooligosaccharides. , xylo-oligosaccharides, stachyose, resistant dextrin, etc., provide effective carbon sources for endogenous probiotics, stimulate their growth and control, while indirectly protecting intestinal health and promoting the absorption of nutrients, which generally do not Produce allergic and other immune reactions. Prebiotics are non-organic living organisms and reach the intestines in undigested for...

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Problems solved by technology

Bifidobacterium infantis, Bifidobacterium adolescentis and Bifidobacterium longum selected by this invention all grow well in the culture medium, but the culture medium also has the ability to promote the synergistic proliferation of Lactobacillus and Bifidobacterium Purpose

[0006] CN107653196A discloses a kind of Paulady's yeast enrichment medium and preparation method thereof, and described Brady's yeast enrichment medium is aseptically prepared with 100 mL In terms of substances contained in water, it contains 4-6 g of glucose, 2-3 g of yeast extract powder, 0.2-0.6 g of fructooligosaccharide, 0.2-0.5 g of disodium hydrogen phosphate, 0.1-0.5 g of calcium chloride, and 1- 3g, corn steep liquor 2-3g, soybean oligosaccharides 0.2-0.5g, xylooligosaccharides 0.3-0.6g, the presence of this medium also fails to achieve the purpose of promoting the synergistic proliferation of Lactobacillus and Bifidobacteria

[0007] CN112063679A discloses a method for mixing probiotics to ferment complex carbohydrates to prepare short-chain fatty acids, wherein the fermentation medium includes the following components in every 100 mL : Carbon source 1~2 g, mucin 0.2~0.6 g, casein 0.2~0.6 g, peptone 0.3~0.7 g, tryptone 0.3~0.7 g and other components, wherein the carbon source is oligosaccharides, non-starch polysaccharides, The mixture of non-toxic starch and sugar alcohol can achieve the purpose of producing short-chain fatty acids in vitro, but the co-cultivation of Bifidobacterium and Lactobacillus cannot be carried out during the culture process, nor can it promote the synergistic proliferation of Lactobacillus and Bifidobacteria the goal of

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