The invention discloses a method for propagating raspberry by
cell culture, which is characterized by comprising the following steps of: selecting young and tender stem segments and stem tips of the raspberry to serve as explants, disinfecting the explants,
cutting the explants into stem segments with axillary buds, inoculating the stem segments with the axillary buds to a sterilized induction culture medium to culture and grow lateral buds, and performing successive transfer culture;
cutting the lateral buds into small blocks, and inoculating the small blocks to a culture medium in a
test tube to culture and grow young leaves; taking a
sprouting seedling out of the
test tube, inoculating the
sprouting seedling to the culture medium to perform rooting culture, opening a culture flask widely for
tempering the
sprouting seedling when adventitious roots grow out on the base of a
bud, and then
transplanting the seedling to a seedling
bed; preparing the seedling
bed with a matrix, taking a test-tube
plantlet out of the culture flask, putting the test-tube
plantlet into
warm water to wash off the culture medium, dipping the test-tube
plantlet into IBA solution, and
transplanting the test-tube plantlet to the seedling
bed; and shading the transplanted test-tube plantlet and preserving the
moisture of the test-tube plantlet during the initial stage of
transplantation, culturing the test-tube plantlet on the seedling bed for 22 to 28 days, transferring the test-tube plantlet into a
nutrition pot, and finally
transplanting the test-tube plantlet into a large farmland. The method propagates seedlings of the raspberry quickly by using a
tissue culture technique, accelerates
desertification control and ecological restoration in China and promotes the development of regional economic constructions.