A kind of culture medium applied to the solid-state fermentation of Trametes to produce laccase

Abstract

The invention relates to technical field of fermentation, in particular to a culture medium applied to production of laccase from solid fermentation of tramete. The culture medicum comprises the following raw materials in percentage by weight: 38-45% of hickory shells, 2-10% of rapeseed dreg powder and the balance of water. The sterilized and cooled culture medium is inoculated into a bran culture, and put in a solid fermentation tray for fermentation. After the tramete is inoculated into the culture medium, the growing period is short, so that the laccase can be rapidly produced, the fermentation period is 5-9 days, and the fermentation time is shortened. Moreover, the lignocellulose can promote the tramete to produce the laccase, so that the production efficiency of the laccase can be greatly improved through the culture medium provided by the invention. The growth of various infectious microbes can be prohibited by polyphenol in the hickory shells, and the tramete can grow in the hickory shells, so that the contamination rate can be reduced because of the culture medium provided by the invention.

Description

technical field [0001] The invention relates to the technical field of fermentation, in particular to a culture medium applied to the solid-state fermentation of Trametes to produce laccase. Background technique [0002] Pine laccase is a copper-containing polyphenol oxidase, which can use oxygen as an electron acceptor to oxidize aromatic compounds and has strong substrate specificity and substrate breadth, so laccase is widely used in the paper industry , environmental protection, food industry and organic synthesis. [0003] Laccases are divided into plant laccases and fungal laccases. Trametes has attracted attention because of its rapid growth and strong enzyme production ability. Fungal laccase is currently mainly obtained through liquid submerged fermentation and solid-state fermentation. Compared with liquid fermentation, solid-state fermentation has the advantages of simple process, low energy consumption, low equipment investment and less waste liquid. The synth...

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Problems solved by technology

[0004] Luo Shaohua et al. (Optimization of laccase-producing conditions for wood chips pretreated by white-rot fungus and evaluation of saccharification, Wine Science and Technology, 2011, 10:17-20) disclosed a laccase-producing medium for the species of Trachelia grandis, composed of wood chips, wheat bran, ammonium tartrate and nutrient solution, the ratio of material to water is 0.28, and the nutrient solution of the medium contains Tween 80, calcium chloride, magnesium sulfate, etc. high oxygen demand

[0005] Wang Xin et al. (The impact of culture conditions on the production of laccase by solid-state fermentation of Trametesversicolor SYBC13, Northwest Agricultural Journal, 2009, 18 (6): 174-179) discloses a solid-state fermentation medium for Trametes versicolor SYBC13, which consists of soybean meal, sawdust , maltose, sodium nitrate, and copper sulfate. The disadvantage of this medium is that copper ions need to be added, which increases the treatment cost of fermentation wastewater

The disadvantage of this invention is that the cultivation time is longer, and it needs to be cultivated for 20-30 days at 20-28°C

The disadvantage of this invention is that due to the wide variety of white rot fungi, the enzyme production conditions of different types of microorganisms in the medium may vary greatly

[0008] Chinese Patent Publication No. CN102851260A, the publication date is January 2, 2013, and the title of the invention is "a method for producing laccase by solid-state fermentation using rice straw as raw material". The culture medium is composed of crushed rice straw. The disadvantage of the invention is that the culture medium needs to adjust the pH, which is troublesome for solid-state fermentation.