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Method for propagating raspberry by cell culture

A technology for cell culture and raspberry is applied in the field of tissue culture of economic shrubs, which can solve the problems of backward method of breeding seedlings, short supply of seedlings and the like, and achieve the effects of easy management, less disease and high economic benefit.

Inactive Publication Date: 2010-07-07
沈阳科奥可再生能源研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the backward breeding of seedlings, the shortage of seedlings has become the main limiting factor for the development of raspberry production

Method used

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Experimental program
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Effect test

Embodiment Construction

[0012] The concrete technical scheme of the present invention will be further described below in conjunction with embodiment:

[0013] Select young raspberry stems and stem tips as explants (preferably in spring), cut off the leaves, soak in washing powder water (concentration about 5%) for 5 minutes, and rinse under tap water for 30-60 minutes (water flow is not suitable) If it is too large), then disinfect it with 70-75% alcohol for 10 seconds in the sterile room, then soak it in 0.1% mercuric chloride solution for 7-10 minutes, rinse it with sterile water 4-5 times, and cut it into 0.5-1.5cm Stem segments with axillary buds were inoculated into sterilized induction medium (MS 改良 +6-BA 2mg / L+NAA 0.45mg / L+sucrose 30g / L+agar 7g / L), and then cultured at 26±1°C, light intensity 1900lx, light time 12h / d. After about 10 days, lateral buds can grow, and when the lateral buds grow to 2-3 cm, subculture can be carried out.

[0014] The lateral buds were cut into small pieces of abo...

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PUM

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Abstract

The invention discloses a method for propagating raspberry by cell culture, which is characterized by comprising the following steps of: selecting young and tender stem segments and stem tips of the raspberry to serve as explants, disinfecting the explants, cutting the explants into stem segments with axillary buds, inoculating the stem segments with the axillary buds to a sterilized induction culture medium to culture and grow lateral buds, and performing successive transfer culture; cutting the lateral buds into small blocks, and inoculating the small blocks to a culture medium in a test tube to culture and grow young leaves; taking a sprouting seedling out of the test tube, inoculating the sprouting seedling to the culture medium to perform rooting culture, opening a culture flask widely for tempering the sprouting seedling when adventitious roots grow out on the base of a bud, and then transplanting the seedling to a seedling bed; preparing the seedling bed with a matrix, taking a test-tube plantlet out of the culture flask, putting the test-tube plantlet into warm water to wash off the culture medium, dipping the test-tube plantlet into IBA solution, and transplanting the test-tube plantlet to the seedling bed; and shading the transplanted test-tube plantlet and preserving the moisture of the test-tube plantlet during the initial stage of transplantation, culturing the test-tube plantlet on the seedling bed for 22 to 28 days, transferring the test-tube plantlet into a nutrition pot, and finally transplanting the test-tube plantlet into a large farmland. The method propagates seedlings of the raspberry quickly by using a tissue culture technique, accelerates desertification control and ecological restoration in China and promotes the development of regional economic constructions.

Description

technical field [0001] The invention relates to tissue culture of shrub economic plants, in particular to a method for cell culture and propagation of raspberries, belonging to bioengineering and rapid breeding technology of shrub economic plants. Background technique [0002] Raspberry is a small perennial deciduous shrub belonging to Rubus genus Rosaceae. The fruit is rich in GABA, vitamins, salicylic acid, antioxidant dismutase tannin and other disease-preventing and anti-cancer drugs. Pain, nourishing muscles and promoting blood circulation, etc., has high economic value. At present, my country has a raspberry cultivation area of ​​about 3000hm 2 , it is predicted that the development space of raspberry in my country is about 50000hm 2 . Due to the backwardness of the existing seedling breeding methods, the shortage of seedlings has become the main limiting factor for the current development of raspberry production. my country's ecological and environmental problems ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 冯连春韩德昌孙维厚杨家鹤高兴安
Owner 沈阳科奥可再生能源研究所
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