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Bacillus megatherium QBJP-F6 and application thereof

A technology of Bacillus megaterium and QBJP-F6, which is applied in the field of agricultural microorganisms, can solve the problems of insufficient systematic and in-depth research and no large-scale promotion products, etc., and achieve the effect of good growth promotion, survival ability and growth promotion.

Active Publication Date: 2017-03-22
南京秦邦吉品农业开发有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, some domestic experts are trying to work in this area, mainly focusing on using AM mycorrhiza to inoculate existing substrates for seedling cultivation. Although some results have been achieved, the research is not systematic and in-depth, nor has it formed a large-scale promotion and has been recognized by the majority of farmers. products, so the research on this patent becomes particularly important

Method used

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  • Bacillus megatherium QBJP-F6 and application thereof
  • Bacillus megatherium QBJP-F6 and application thereof
  • Bacillus megatherium QBJP-F6 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1, isolation and identification of bacterial strain

[0033] Take 5g of soil in a triangular flask filled with 45ml of sterile water, and shake it in a shaker at 30°C and 170r / min for 20min to make a soil suspension. After 20min in a water bath at 80°C, dilute to 10 -1 、10 -2 、10 -3 、10-4 、10 -5 After waiting for different gradient dilutions, smear the plate, incubate in the dark for 1d to 2d in the incubator, and then pick a single colony, after purification on the plate, select 12 strains in total, and measure the ability of each strain to produce the auxin indole acetic acid (IAA) (Method reference: Glickmann E, DessauxY. A critical examination of the specificity of the salkowski reagent forindolic compounds produced by phytopathogenic bacteria. [J]. Applied and environmental microbiology, 1995, 612). Using the same method, a strain QBJP-F6 with excellent growth-promoting ability was obtained as a result of primary screening and re-screening, which was ...

Embodiment 2

[0036] Embodiment 2, the production of functional bacteria bacterial liquid

[0037] The strain QBJP-F6 (CGMCC NO.12888) was inoculated into the PDA culture medium for liquid fermentation production. The conditions for fermentation production were: pH 7.0, fermentation temperature 30°C, stirring speed 170rpm / min, spores formed in the middle and late stages of fermentation, The fermentation time is 48 hours, so that the amount of bacteria or spores in the fermentation broth is ≥1×10 10 a / ml;

[0038] The preparation method of the PDA culture medium used is as follows, taking the preparation of 1L medium as an example: 200g potatoes are peeled, cut into small pieces, put them in water and boil them, boil them for 30min and then filter them, add 20g ordinary sucrose to the filtrate and make the volume to 1000ml , natural pH, sterilized at 121°C for 20min.

Embodiment 3

[0039] Example 3, research and development of functional biological matrix

[0040] The effect of biological matrix seedling cultivation with strain QBJP-F6 as functional bacteria

[0041] Research and development of the substrate: Inoculate the fermentation broth of the bacterial strain QBJP-F6 prepared in Example 2 (inoculate according to 5v / w% of the dry weight of the substrate, the same below) into the common seedling cultivation substrate, and mix evenly. The functional strain QBJP-F6 in the matrix was counted by selective medium. The formula of selective medium was: peptone 10.0g, yeast powder 5.0g, NaCl 10.0g, agar 2.0%, deionized water 1000mL, pH 7.2-7.4, 121 ℃ autoclave for 20min. 1% polymyxin 2mL, 1% cycloheximide 4mL (the antibiotics are added after the medium is cooled before being poured). The number of colonies of functional bacteria QBJP-F6 is calculated by dry weight per gram of substrate. After counting, the number of functional bacteria in the biological m...

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Abstract

The invention discloses a bacillus megatherium QBJP-F6. The bacillus megatherium QBJP-F6 is preserved in the Common Microorganism Center of the China Committee for Culture Collection of Microorganisms, and the preservation number is CGMCC NO.12888. The invention further discloses an application of the bacillus megatherium QBJP-F6 in preparation of a functional vegetable seedling biological matrix. The QBJP-F6 can effectively colonize the rhizosphere of a seedling, and a bacterial strain has a high growth promoting function and can enhance growth in seedling later-period potting and field planting. The functional vegetable seedling biological matrix has good growth promoting effect on vegetables at the seedling stage and can significantly promote growth of various vegetables at the seedling stage, high-quality seedlings of which the rhizospheres are provided with a large number of active function microorganisms can be obtained, and growth of the seedlings in the later-period cultivation process can be significantly promoted.

Description

technical field [0001] The invention relates to the field of agricultural microorganisms, in particular to a bacillus megaterium QBJP-F6 and its application in preparing functional vegetable seedling-raising biological substrates. Background technique [0002] With the development of my country's modern agriculture, more and more people pay more and more attention to industrial vegetable seedling cultivation, and plug seedling cultivation is the main measure of current industrial seedling cultivation. According to incomplete statistics, the area of ​​crops that need to be raised in my country is very large, among which the planting area of ​​rice exceeds 300 million mu / year, and the area of ​​economic crops exceeds 500 million mu / year. At present, in China, materials rich in organic matter (such as peat, decomposed plant residues, etc.) are mainly used, and an appropriate proportion of light inorganic materials, such as vermiculite and expanded perlite, are used to make seed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01G9/10C12R1/11
CPCA01G24/00C12N1/205C12R2001/11
Inventor 秦俊巩子毓俞萍李荣
Owner 南京秦邦吉品农业开发有限公司
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