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Bacillus subtilis strain and application thereof in aquaculture

A technology of Bacillus subtilis and Bacillus licheniformis, applied in the application, Lactobacillus, Bifidobacterium and other directions, can solve the problems affecting the food safety of aquatic products, aquatic animal residues, drug-resistant strains, etc., to reduce the occurrence of animal diseases , the effect of efficient decomposition and good enzyme production capacity

Active Publication Date: 2020-09-22
青岛玛斯特生物技术有限公司 +1
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the application of antibiotics has also brought many side effects to humans and the breeding industry, and it is becoming more and more serious, which has attracted the attention of many parties.
The use of antibiotics is prone to the problem of drug-resistant strains, and there is a problem of residues in aquatic animals, which affects the food safety of aquatic products, so antibiotic substitution has become inevitable

Method used

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  • Bacillus subtilis strain and application thereof in aquaculture
  • Bacillus subtilis strain and application thereof in aquaculture
  • Bacillus subtilis strain and application thereof in aquaculture

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Isolation, screening and identification of embodiment 1 bacterial strain

[0026] 1. Sample

[0027] The intestines of Litopenaeus vannamei were collected from the shrimp farm in Jiaozhou, Qingdao.

[0028] 2. Screening method

[0029] Take the intestinal tract of prawns, add sterile saline, shake at 28°C, 150rpm for 1 hour, so that the bacteria are fully dispersed in the saline, 10-fold gradient dilution, inoculated on 2216E, TSB and MRS solid medium respectively, and culture at 28°C 24h-48h. Select a uniform and clear single colony and streak to purify the bacteria. The obtained single colonies were named DN01, DN02, DN03, ..., DN32 accordingly.

[0030] 3. Analysis of biological characteristics of symbiotic bacteria in shrimp digestive tract

[0031] The symbiotic bacteria (test strain) and pathogenic bacteria (control strain) were activated and cultured on an inclined plane, and an appropriate amount of normal saline was added to make a concentration of 10 4-7...

Embodiment 2

[0045] Example 2 Bacillus subtilis DN02 Enzyme Production Ability Determination

[0046] 1. Qualitative experiment on enzyme production ability of potential probiotics

[0047] Prepare protease-producing qualitative medium, amylase-producing qualitative medium, and cellulase-producing qualitative medium respectively, activate the screened antagonistic bacteria of pathogenic bacteria, and inoculate the liquid culture of each strain on different culture mediums with sterile inoculation sticks. On the base, cultivate at constant temperature for 48 hours, and observe after the formation of obvious colonies. When the strain produces protease to decompose casein, a transparent circle appears on the plate; the starch medium needs to be added with Lugol's iodine solution, and the strain produces amylase, and a transparent circle appears on the blue-purple plate; the qualitative medium producing cellulase needs to be stained with Congo red , and then washed with NaCl, the colony produ...

Embodiment 3

[0058] Embodiment 3 Bacillus subtilis DN02 growth performance evaluation

[0059] Inoculate Bacillus subtilis DN02 into the liquid fermentation medium according to the inoculation amount of 1%, 30°C, 180r / min, shake culture, take samples every 2h, measure the OD 600 value of the bacterial liquid, see the specific results figure 2 .

[0060] like figure 2 As shown, the growth curve of Bacillus subtilis DN02 changes in stages, and its lag period is short. After 4 hours, it enters the exponential growth period, and the OD value shows a rapid growth trend, and it is in a stable period for 10-28 hours. At the same time, the bacterium produced spores when cultured for 8 hours, and the concentration of spores reached 10 8 CFU / mL. The characteristics of short growth lag period and long stable period of DN02 are very suitable for the development of fermentation industry.

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Abstract

The invention relates to the technical field of functional microorganism screening and application, in particular to novel bacillus subtilis and application thereof in aquaculture. The bacillus subtilis is screened from intestinal tracts of litopenaeus vannamei, the preservation number is CCTCC NO: M 2020091, pathogenic bacteria can be significantly inhibited, the immunity and disease resistance of aquatic animals are improved, protease and amylase are produced to improve feed digestion and utilization, and the growth of the aquatic animals is promoted, and the bacillus subtilis can be widelyapplied to the field of aquatic feeds.

Description

technical field [0001] The invention relates to the technical field of functional microorganism screening, in particular to a strain of Bacillus subtilis and its application in aquaculture. Background technique [0002] my country is a large fishery country, and aquaculture has become one of the main sources of food supply in my country. Aquatic products have the characteristics of high nutritional value, delicious taste, and beneficial to the human body. With the rapid development of the national economy and the continuous improvement of living standards, people are interested in The demand for aquatic products is increasing day by day. At the same time, the internationalization of aquatic product trade has promoted the continuous expansion of the scale of aquaculture in my country. However, the aquaculture model dominated by high-density and intensive farming has produced many problems, such as water eutrophication, poor water quality, and outbreaks of various diseases, whi...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34A23K10/18A23K50/80C12R1/125C12R1/10C12R1/07C12R1/25C12R1/23C12R1/01C02F103/20C02F101/16
CPCC12N1/20C02F3/341A23K10/18A23K50/80C02F2103/20C02F2101/16C02F2101/166C12R2001/125C12N1/205A23V2400/113A23V2400/169A23V2400/51
Inventor 周怡王磊倪梦丽郭本月刘广魏万权
Owner 青岛玛斯特生物技术有限公司
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