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Microbial preparation for preventing and controlling plant root knot nematodes and application thereof

A technology for microbial preparations and nematodes, which is applied in the directions of plant growth regulators, biocides, nematicides, etc., can solve the problems of restricting the development and application of Paecilomyces lilacinus, insufficient control effects, and increased production costs. Conducive to large-scale promotion, significant control of root-knot nematodes, and simple production and operation

Active Publication Date: 2016-10-12
山东鲁虹智慧农业研究院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Because Paecilomyces lilacinus grows in a wide range of temperatures, it can germinate and grow at 8-38 ° C, and it is easy to die at high temperatures, so it is often affected by storage conditions in practical applications, which greatly limits the growth of Paecilomyces lilacinus Develop application
[0006] The existing Paecilomyces lilacinus preparations are produced through various processes, adding various solvents, stabilizers, additives, etc., the method is complicated, and the addition of other auxiliary components increases the production cost, and the control effect is not good enough

Method used

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  • Microbial preparation for preventing and controlling plant root knot nematodes and application thereof
  • Microbial preparation for preventing and controlling plant root knot nematodes and application thereof
  • Microbial preparation for preventing and controlling plant root knot nematodes and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment １

[0036] Stability test of Paecilomyces lilacinus

[0037] Take a certain amount of Paecilomyces lilacinus fungus and divide it into 4 parts, put them in a dry environment at 25°C, 30°C, 35°C, and 40°C respectively, take samples at regular intervals and use the gradient dilution method to detect the survival of spores, so as to initially determine the Paecilomyces lilacinus fungus. The storage temperature of Penicillium is shown in Table 1 and Table 2 for details.

[0038] Table 1 Preservation of Paecilomyces lilacinus at 25°C, 30°C and 35°C

[0039] .

[0040] Table 2 Preservation of Paecilomyces lilacinus at 40°C

[0041] .

[0042] The test results in Table 1 and Table 2 show that after Paecilomyces lilacinus was preserved for 9 days at 25°C, the spore survival rate remained above 75%; as the temperature increased, the spore survival rate decreased; when stored at 40°C for 24 Hours later the spore survival rate dropped to 73%. Preliminary conclusions were drawn that ...

Embodiment 2

[0044] Preparation of microbial preparations for controlling plant root-knot nematodes

[0045] Prepare each composition and content of the microbial preparation 100 g of preventing and controlling plant root-knot nematode of the present invention and content as follows:

[0046] Paecilomyces lilacinus agent: 2 g,

[0047] Bacillus subtilis inoculum: 2 g,

[0048] Shell powder: 2 g,

[0049] Shrimp shell powder: 2 g,

[0050] Weathered coal humic acid: 50 g,

[0051] Diatomaceous earth: 42 g.

[0052] Weigh each component according to the formula, mix them together, stir evenly, and seal them for storage.

Embodiment 3

[0054] Preparation of microbial preparations for controlling plant root-knot nematodes

[0055] Prepare each composition and content of the microbial preparation 100 g of preventing and controlling plant root-knot nematode of the present invention and content as follows:

[0056] Paecilomyces lilacinus agent: 4 g,

[0057] Bacillus subtilis inoculum: 4 g,

[0058] Shell powder: 2 g,

[0059] Shrimp shell powder: 2 g,

[0060] Weathered coal humic acid: 50 g,

[0061] Diatomaceous earth: 38 g.

[0062] Weigh each component according to the formula, mix them together, stir evenly, and seal them for storage.

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Abstract

The invention discloses a microbial preparation for preventing and controlling plant root knot nematodes and the application thereof. The preparation is mixed from Paecilomyces lilacinus, Bacillus subtilis, shell powder, shrimp shell powder, weathered coal humic acid and peat. The shell powder and shrimp shell powder are added to induce paecilomyces lilacinus to produce chitinase. The preparation can be stored at room temperature, and after storage for 90 days at 25 DEG C, the survival rate of paecilomyces lilacinus is more than 80%. The tomato pot experiment and cucumber field test prove that the preparation has remarkable effect on the control of root knot nematodes; at the same time, soil colonization test verify the Paecilomyces lilacinus in the preparation has good colonization condition in soil.

Description

technical field [0001] The invention relates to the technical field of biological control, in particular to a microbial preparation for controlling plant root-knot nematode and its application. Background technique [0002] Plant nematode diseases are diseases caused by the invasion and parasitism of plant parasitic nematodes. The survey found that there are more than 3000 kinds of plants or crops affected by nematode diseases. Mainly food crops, such as wheat, sweet potato, rice; vegetable crops, tomato, pepper, spinach; and other crops. Plant nematode diseases often occur in our country and are seriously harmful, and the area of ​​occurrence has expanded from time to time, especially in heavy crops, root-knot nematodes are particularly serious. After the nematode invades the plant, the normal growth and development of the plant is persecuted, thereby reducing the yield and quality. Nematode control has always been an urgent problem in agricultural production. [0003] ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01N63/00A01N61/00A01P5/00
CPCA01N61/00A01N63/00A01N63/30A01N2300/00
Inventor 黄道跟杨春玉阎鹏高超李恒
Owner 山东鲁虹智慧农业研究院
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