Trichoderma harzianum-contanining functional vegetable seedling biological matrix and preparation method thereof
A seedling-raising bio-substrate and seedling-raising substrate technology, applied in the field of functional vegetable seedling-raising bio-substrate and its preparation, can solve the problems of indeterminate growth-promoting effect and inconspicuous growth-promoting effect, and achieve enhanced growth and prevention and control of soil-borne diseases, Enhance the effect of preventing and controlling soil-borne diseases and promoting growth
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Embodiment 1
[0028] Embodiment 1, the production of functional bacteria liquid
[0029] Inoculate the strain SQR-T037 (CGMCC NO.3308) into the PDA culture medium for liquid fermentation production. The conditions for fermentation production are: natural pH, culture temperature 28-30°C, stirring speed 170-300 rpm, and the formation of Spores, so that the amount of spores in the fermentation broth is ≥5×10 8 pieces / ml. The preparation method of the PDA culture medium used is as follows, taking the preparation of 1L medium as an example: Peel 200g of potatoes, cut into small pieces, put them in water and boil, boil for 30min, add 20g of ordinary sucrose to the filtrate after filtration, and dilute to 1000ml, adjust the pH value to 7.0-7.6, and sterilize at 121°C for 20min.
Embodiment 2
[0030] Embodiment 2, research and development of functional biomatrix
[0031] 2.1 Seedling raising effect of different inoculated amount of biological matrix
[0032] Research and development of the substrate: The strain SQR‐T037 was inoculated into the common seedling cultivation substrate with 2% and 5% of the inoculation amount respectively, mixed well, and counted to contain 0.52×10 7 CFU / g, 2.19×10 7 CFU / g dry weight of functional bacteria. SQR‐T037 in the matrix was counted using selective medium: glucose 3g, NH 4 NO 3 1g, KH 2 PO 4 0.9g, MgSO 4 ·7H 2O0.2g, KCl0.15g, Bengal red 0.15g. Deionized water 1000ml, sterilized at 115°C for 30min. Add chloramphenicol 0.25g after medium cooling, streptomycin 9ml (1%w / v), propamocarb 1.2ml (772g / L), pentachloronitrobenzene 0.2g (Williams J., Clarkson J.M., Mills P.R., et al. A selective medium for quantitative reisolation of Trichoderma harzianum from Agaricus bisporus compost [J], Applied and environmental microbiology,...
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