Trichoderma harzianum-contanining functional vegetable seedling biological matrix and preparation method thereof

A seedling-raising bio-substrate and seedling-raising substrate technology, applied in the field of functional vegetable seedling-raising bio-substrate and its preparation, can solve the problems of indeterminate growth-promoting effect and inconspicuous growth-promoting effect, and achieve enhanced growth and prevention and control of soil-borne diseases, Enhance the effect of preventing and controlling soil-borne diseases and promoting growth

Inactive Publication Date: 2014-05-07
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Microbial organic fertilizers have the effect of disease prevention and growth promotion, but because of the high salt content of the bio-organic fertilizer, Ec>6.0ms / cm (the ratio of fertilizer to water is equal to 1:5, the data measured by the Ec determination method of the same substrate), while the seedlings are resistant to salt Concentration-sensitive specific biological characteristics lead to fertilizers that cannot be used for seedling growth, and the growth-promoting effect of adding microorganisms directly to the soil is not obvious. Therefore, those skilled in the art cannot determine whether adding it to the seedling-raising substrate can play a role in promoting growth effect

Method used

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  • Trichoderma harzianum-contanining functional vegetable seedling biological matrix and preparation method thereof
  • Trichoderma harzianum-contanining functional vegetable seedling biological matrix and preparation method thereof
  • Trichoderma harzianum-contanining functional vegetable seedling biological matrix and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Embodiment 1, the production of functional bacteria liquid

[0029] Inoculate the strain SQR-T037 (CGMCC NO.3308) into the PDA culture medium for liquid fermentation production. The conditions for fermentation production are: natural pH, culture temperature 28-30°C, stirring speed 170-300 rpm, and the formation of Spores, so that the amount of spores in the fermentation broth is ≥5×10 8 pieces / ml. The preparation method of the PDA culture medium used is as follows, taking the preparation of 1L medium as an example: Peel 200g of potatoes, cut into small pieces, put them in water and boil, boil for 30min, add 20g of ordinary sucrose to the filtrate after filtration, and dilute to 1000ml, adjust the pH value to 7.0-7.6, and sterilize at 121°C for 20min.

Embodiment 2

[0030] Embodiment 2, research and development of functional biomatrix

[0031] 2.1 Seedling raising effect of different inoculated amount of biological matrix

[0032] Research and development of the substrate: The strain SQR‐T037 was inoculated into the common seedling cultivation substrate with 2% and 5% of the inoculation amount respectively, mixed well, and counted to contain 0.52×10 7 CFU / g, 2.19×10 7 CFU / g dry weight of functional bacteria. SQR‐T037 in the matrix was counted using selective medium: glucose 3g, NH 4 NO 3 1g, KH 2 PO 4 0.9g, MgSO 4 ·7H 2O0.2g, KCl0.15g, Bengal red 0.15g. Deionized water 1000ml, sterilized at 115°C for 30min. Add chloramphenicol 0.25g after medium cooling, streptomycin 9ml (1%w / v), propamocarb 1.2ml (772g / L), pentachloronitrobenzene 0.2g (Williams J., Clarkson J.M., Mills P.R., et al. A selective medium for quantitative reisolation of Trichoderma harzianum from Agaricus bisporus compost [J], Applied and environmental microbiology,...

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Abstract

The invention provides a trichoderma harzianum-contanining functional vegetable seedling biological matrix and a preparation method thereof. The functional vegetable seedling biological matrix is formed by adding a functional bacterial strain, namely the trichoderma harzianum SQR-T037, into a common seedling matrix. As for seedlings such as cucumbers, eggplants and watermelons, the specific fungus trichoderma harzianum SQR-T037 is selected, is kept alive and is added into the matrix to obtain an active biological matrix product, and by using the product, the plant growth promoting effect on seedling stages of seedlings such as the cucumbers, eggplants and watermelons is good, and high-quality seedlings of which the rhizospheres include a large amount of microorganisms with an activity function can be cultivated; meanwhile, the beneficial microorganisms can be in large-scale colonization at the rhizospheres of the seedlings, so that the growth of the cultivated seedlings in the later cultivation process can be promoted, the yield of the crops planted in a big field is improved, and the effect of preventing and controlling a cucumber soil-borne disease of the cultivated seedlings is achieved.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and relates to a functional vegetable seedling-raising biological matrix containing Trichoderma harzianum and a preparation method thereof. Background technique [0002] With the development of facility agriculture at home and abroad, more and more attention has been paid to industrialized seedling cultivation. According to incomplete statistics, the area of ​​crops that need to be raised in my country is very large, among which the planting area of ​​rice exceeds 300 million mu / year, and the area of ​​economic crops exceeds 500 million mu / year. At present, in China, materials rich in organic matter (such as peat, decomposed plant residues, etc.) are mainly used, and an appropriate proportion of light inorganic materials, such as vermiculite and expanded perlite, are used to make seedling substrates. Since the source of materials is limited by region and cost, and peat is a non-renewab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C12N1/14C12R1/885
Inventor 沈其荣张苗李荣吕娜娜王康徐阳春
Owner NANJING AGRICULTURAL UNIVERSITY
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