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Application of bacteroides fragilis and zwitterionic capsular polysaccharide thereof in preparation of medicine for treating respiratory system tumors

A technology of Bacteroides fragilis and zwitterions, applied in the field of biomedicine, can solve problems such as the absence of microecological products for the treatment of respiratory system tumors

Active Publication Date: 2022-04-29
GUANGZHOU ZHIYI PHARMA INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] An important document published in Nature in 2017 proposed "microecological regulation of the gut-lung axis", confirming that intestinal flora is closely related to tumors. However, there is no microecological product for the treatment of respiratory tumors in the prior art

Method used

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  • Application of bacteroides fragilis and zwitterionic capsular polysaccharide thereof in preparation of medicine for treating respiratory system tumors
  • Application of bacteroides fragilis and zwitterionic capsular polysaccharide thereof in preparation of medicine for treating respiratory system tumors
  • Application of bacteroides fragilis and zwitterionic capsular polysaccharide thereof in preparation of medicine for treating respiratory system tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Embodiment 1: the fermentation culture of Bacteroides fragilis

[0070] Streak inoculation of Bacteroides fragilis ZY-312 strain on blood plate, anaerobic culture for 48h. Observe the colony morphological characteristics, staining characteristics, size, club shape and distribution, etc. Colony characteristics: Bacteroides fragilis ZY-312, after being cultured on a blood plate for 48 hours, is slightly convex, translucent, white, smooth, non-hemolytic, and the diameter of the colony is between 1-3mm. See figure 1 .

[0071] Morphology under the microscope: Bacteroides fragilis ZY-312 was examined by Gram staining. It is a Gram-negative bacterium with a typical rod shape, blunt rounded ends and dense staining. The uncolored part in the middle of the bacteria is like a vacuole. figure 2 .

Embodiment 2

[0072] Embodiment 2 Bacteroides fragilis living bacteria liquid and the preparation of inactivated bacteria liquid

[0073] 1) Preparation of live bacteria solution

[0074] Select the single bacterium colony cultivated in Example 1 and inoculate it into the plant-derived peptone liquid medium for fermentation and cultivation for 8 hours (at a temperature of 37° C.) to obtain a live Bacteroides fragilis liquid; After 15 minutes, the supernatant was removed, and the precipitate was collected to obtain the Bacteroides fragilis ZY-312 sludge.

[0075] 2) Preparation of inactivated bacteria solution

[0076] The above bacterial liquid was taken and subjected to conventional heat inactivation treatment to obtain the inactivated bacterial liquid of Bacteroides fragilis ZY-312.

Embodiment 3

[0077] Example 3: Preparation of Bacteroides fragilis capsular polysaccharide

[0078] The bacteria slime prepared in Example 1 was used to carry out the experiment.

[0079] (1) Take 50g of bacteria slime, add 300g of purified water to resuspend the bacteria, adjust its pH to 3.5 with 1mol / L hydrochloric acid solution, extract at 100°C for 1.5h, cool to room temperature, centrifuge at 12000g at room temperature for 10min, take the supernatant to obtain rough sugar solution;

[0080] (2) The crude sugar solution is concentrated by ultrafiltration through a 10KD ultrafiltration membrane to remove small molecular impurities until the conductivity is stable, and the reflux liquid is collected;

[0081] (3) Add an equal volume of 40mmol / L Tris-HCl (pH8.5) to the reflux liquid to convert the salt; .5, containing 0.2mol / L NaCl) gradient elution for 25 column volumes, segmented collection, 100mL / bottle (component), SEC-HPLC tracking monitoring, combined 206nm absorption peak as a s...

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Abstract

The invention discloses an application of Bacteroides fragilis and / or zwitterionic capsular polysaccharide thereof in preparation of a medicine for treating respiratory system tumors, the Bacteroides fragilis, especially the Bacteroides fragilis ZY-312 with the preservation number of CGMCC No.10685 and the zwitterionic capsular polysaccharide thereof, can be used for preparing medicines for treating respiratory system tumors by increasing the levels of anti-tumor factors IL-12 and IFN-gamma, and can be used for preparing medicines for treating respiratory system tumors. The traditional Chinese medicine composition can be used for inhibiting expression of a tumor promoting factor IL-1beta, promoting up-regulation of the proportion of infiltrated CD8 + CD45 + T cells in tumors, regulating the microenvironment of tumor tissues, reducing the weight of in-situ tumors and inhibiting the number of tumor metastases, can be used for effectively preventing and treating respiratory system tumors, can be independently used for cancer treatment, and also can be used for preventing and treating tumors. The traditional Chinese medicine composition can also be combined with other treatment means such as microorganisms, operations, radiotherapy and chemotherapy, so that the comprehensive curative effect is remarkably improved, the harm of radiotherapy and chemotherapy to organisms is relieved, occurrence, development, relapse and metastasis of respiratory system tumors are effectively prevented, and the living quality of patients is improved.

Description

[0001] The microbial strains used in the implementation of the present invention have been preserved on April 2, 2015 at the General Microbiology Center (CGMCC) of the China Microbiological Culture Collection Management Committee (No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing). Taxonomic designation: Bacteroides fragilis ZY-312 (bacteroides fragilis ZY-312), deposit number CGMCC No.10685. Bacteroides fragilis ZY-312 was isolated and obtained by the applicant unit of the present invention, and has been authorized for patent protection (patent number 201510459408.X). According to the provisions of the patent examination guidelines, the public can buy it from commercial channels or has authorized it without preservation, that is, No deposit certificate is required. technical field [0002] The invention relates to the field of biomedicine, in particular to the application of Bacteroides fragilis and / or its zwitterionic capsular polysaccharide in the preparation of d...

Claims

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Application Information

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IPC IPC(8): A61K35/741A61K31/715A61K45/06A61P35/00A61P35/04C08B37/00A23L33/135A23L33/125A23C9/16A23C9/156A23C9/152A23C19/09A23G9/36A23G9/34A23L7/104A23K10/18A23K20/163
CPCA61K35/741A61K31/715A61K45/06A61P35/00A61P35/04C08B37/0003A23L33/135A23L33/125A23C9/16A23C9/156A23C9/152A23C19/09A23G9/363A23G9/34A23L7/104A23K10/18A23K20/163A23V2002/00A61K2300/00A23V2200/308A23V2200/314A23V2250/51Y02A50/30
Inventor 王薇蔡琰郑丽君梁德宝
Owner GUANGZHOU ZHIYI PHARMA INC
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