Polypeptide for promoting apoptosis of breast cancer cells by targeted uptake of siRNA

Abstract

The invention relates to the technical field of biological control of breast cancer disease and discloses a polypeptide for promoting apoptosis of breast cancer cells by targeted uptake of siRNA. Thepolypeptide comprises a polypeptide 1; the sequence of the polypeptide 1 is H-Ile-Phe-D-Trp-Leu-Leu-Trp-Gln-Gly-Arg-Gly-Gly-Gly-Arg-Arg-Arg-Arg-Arg-Arg-Arg-OH. A detection method for the polypeptide for promoting the apoptosis of the breast cancer cells by targeted uptake of the siRNA comprises the following steps: firstly, culturing the breast cancer cells: selecting breast cancer cells MDA-MB-231, culturing the breast cancer cells MDA-MB-231 by adopting a DMEM culture medium, and sequentially adding 10 percent of fetal calf serum, 100 unit / ml of penicillin and 100g / mL of streptomycin in theculture medium. According to the polypeptide for promoting the apoptosis of the breast cancer cells by targeted uptake of the siRNA, the polypeptide 1 wraps the siRNA to form nanoparticles for targeting delivery of the siRNA to the breast cancer cells; the nanoparticles can realize the targeting delivery of the siRNA through a surface receptor of the breast cancer cells and have little damage to normal cells, and the practicality of the polypeptide is improved; meanwhile, the targeting delivery of TRPC1 siRNA by using the polypeptide 1 causes the apoptosis of the breast cancer cells, so that the effect of treating breast cancer is realized.

Description

technical field [0001] The invention relates to the technical field of biological prevention and treatment of breast cancer, in particular to a polypeptide for targeting and introducing siRNA to promote apoptosis of breast cancer cells. Background technique [0002] At present, the treatment of breast cancer includes five major blocks: surgery, chemotherapy, endocrine therapy, radiotherapy and molecular targeted therapy. The development of molecular biology and the in-depth understanding of the pathogenesis at the cellular and molecular levels have brought tumor therapy into a new era of molecular targeted therapy. Compared with chemotherapy and radiotherapy, molecular targeted therapy efficiently and selectively kills tumor cells, making the treatment more targeted and with less side effects. [0003] At present, targeted delivery of siRNA to specific cells has great potential in the field of drug development. siRNA can not only be used as a research tool to inhibit the ex...

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the present invention provides a polypeptide for targeted introduction of siRNA to promote apoptosis of breast cancer cells, which solves the problems that the existing targeted delivery of siRNA is inconvenient and easy to cause damage to normal cells