Ferment method of producing fuel ethanol using stalk material
A technology of fuel ethanol and fermentation method, applied in the direction of biofuel, fermentation, fuel, etc., can solve the problems of waste of resources, environmental pollution, low utilization level, etc., and achieve the effect of low cost
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Embodiment 1
[0018] The fermentation method for producing fuel ethanol from straw raw materials in this embodiment comprises the following steps:
[0019] (1) Selection of raw materials: crop straw, wheat straw, corn straw, rice straw;
[0020] (2) Pulverization: the stalks and straws are pulverized by a hammer mill and sieved into 20-40 mesh rice straw powder;
[0021] (3) Pretreatment: The raw material is subjected to lignin degradation treatment by white-rot fungus under solid-state culture and liquid crude enzyme;
[0022] (4) Double-enzyme saccharification: use cellulase and xylanase to saccharify straw under the conditions of 45°C, pH 4.5, 150rpm, and a liquid-solid ratio of 15 to obtain reducing sugars;
[0023] (5) Fermentation: use Pachysolen tannophilus As2.1585 to co-ferment pentose and hexose sugars in the straw saccharification solution to produce fuel ethanol, wherein the fermentation system is constructed as follows: add the following substances and their dosage to the stra...
Embodiment 2
[0026] The fermentation method for producing fuel ethanol from straw raw materials in this embodiment comprises the following steps:
[0027] (1) Selection of raw materials: crop straw, wheat straw, corn straw, rice straw;
[0028] (2) Pulverization: the stalks and straws are pulverized by a hammer mill and sieved into 20-40 mesh rice straw powder;
[0029] (3) Pretreatment: The raw material is subjected to lignin degradation treatment by white-rot fungus under solid-state culture and liquid crude enzyme;
[0030] (4) Double-enzyme saccharification: use cellulase and xylanase to saccharify straw under the conditions of 45°C, pH 4.5, 150rpm, and a liquid-solid ratio of 15 to obtain reducing sugars;
[0031] (5) Fermentation: use Pachysolen tannophilus As2.1585 to co-ferment pentose and hexose sugars in the straw saccharification solution to produce fuel ethanol, wherein the fermentation system is constructed as follows: add the following substances and their dosage to the stra...
Embodiment 3
[0034] The fermentation method for producing fuel ethanol from straw raw materials in this embodiment comprises the following steps:
[0035] (1) Selection of raw materials: crop straw, wheat straw, corn straw, rice straw;
[0036] (2) Pulverization: the stalks and straws are pulverized by a hammer mill and sieved into 20-40 mesh rice straw powder;
[0037] (3) Pretreatment: The raw material is subjected to lignin degradation treatment by white-rot fungus under solid-state culture and liquid crude enzyme;
[0038] (4) Double-enzyme saccharification: use cellulase and xylanase to saccharify straw under the conditions of 45°C, pH 4.5, 150rpm, and a liquid-solid ratio of 15 to obtain reducing sugars;
[0039] (5) Fermentation: use Pachysolen tannophilus As2.1585 to co-ferment pentose and hexose sugars in the straw saccharification solution to produce fuel ethanol, wherein the fermentation system is constructed as follows: add the following substances and their dosage to the stra...
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