Method for preparing biogas by degrading coal with microorganisms

A technology of microbial degradation and biogas, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as coalbed methane depletion, and achieve the effects of low cost, reduced development cost, and increased output

Inactive Publication Date: 2012-06-27
HENAN POLYTECHNIC UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to technical limitations, most of the currently recoverable coal seams are permeabl

Method used

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  • Method for preparing biogas by degrading coal with microorganisms

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Embodiment

[0034] Embodiment: A kind of method utilizing microbial degradation coal to produce biogas, concrete steps are as follows:

[0035] (1) Collect data, take coal samples and water samples at the same time, and conduct anaerobic bacteria detection on the water samples taken.

[0036] (2) Using the water sample of the mined well as the source of bacteria, in an anaerobic glove box, use the culture medium of methanogens, hydrogen-producing acetogenic bacteria, anaerobic cellulolytic bacteria and sulfate-reducing bacteria to separate and count , to clarify the type of original microorganisms and carry out selective culture. The specific medium formula used is as follows:

[0037] 1) Culture medium for anaerobic hydrogen-producing acetogenic bacteria:

[0038] Glucose 25g, tryptone 3g, beef extract 2.5g, yeast juice 1.5g, NaCl 5g, K 2 HPO 4 1g, MgCl 2 0.4g, FeSO 4 ·7H 2 O 0.3g, L-cysteine ​​0.5g, pH 7.0-7.2

[0039] 2) Anaerobic cellulolytic bacteria culture medium:

[0040...

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Abstract

The invention relates to technology for preparing biogas by degrading coal with microorganisms. The technology is suitable for any environment with oxygen or without oxygen. Under the condition of room temperature (15 to 35 DEG C), biologic methane can be ensured to be continuously generated without activating a strain in a way of raising temperature, so the development cost is effectively reduced; the adopted white-rot fungi is highly adaptive to the environment and is low in cost, so the technology has the feasibility of project development; and different types of colonies can be more effectively used and the effect of the colonies can be improved, so the degradation rate of a coal body is increased, and the yield of biological methane is improved.

Description

technical field [0001] The invention relates to a method for producing biogas by using microorganisms to degrade coal. Background technique [0002] Coalbed methane, also known as coalbed methane, is a new type of clean energy and an important potential alternative energy source in my country. After more than 30 years of exploration and development of coalbed methane, large-scale commercial development has been realized in the United States. In 2007, the output of coalbed methane in the United States exceeded 60 billion cubic meters. After more than 20 years of difficult exploration, my country has realized partial commercial development in Qinshui Basin and Hedong Coalfield in Shanxi, which indicates that this clean energy will soon become an important supplement to my country's primary energy and will improve my country's energy structure. play a driving role. [0003] Coalbed methane has large reserves in the world. However, according to the research of Flores et al., th...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P5/02C12R1/645
CPCY02E50/343Y02E50/30
Inventor 苏现波龚红梅郭红玉林晓英夏大平徐影吴昱陈鑫常天俊潘文维于洪飞
Owner HENAN POLYTECHNIC UNIV
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