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Method for preparing efficient probiotic preparation from macrofungi bacterial liquid (mycoplasm)

A high-efficiency, bacterial preparation technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of low stability of bacterial activity and low therapeutic effect, so as to improve the effect of health care and treatment, The production process is simple and the effect of a wide range of sources

Inactive Publication Date: 2015-01-21
HUNAN MINKANG BIOTECH RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, in the market of probiotic preparations, there are also problems of low activity and stability of the strains and low therapeutic effect; in order to better tap the potential effect of probiotics, in recent years, while exploring the mechanism of action of probiotics at home and abroad , to strengthen the development and research of high-efficiency products, including: (1) screening and combination of microbial varieties, seeking their own bacterial sources (including screening of genetically engineered strains), and developing compound bacterial preparations (because of the synergistic effect of compound bacterial preparations compared with single bacterial preparations) (2) Research on the stability of probiotics products, in the screening of excellent strains, the fermentation process of high-activity bacteria agents, the protection of bacteria (microcapsule coating, etc.) and synergists Breakthroughs have been made in key technologies such as selection, so that the activity of the bacteria can be maintained during preservation and processing; (3) The development of prebiotics, exploring new prebiotics, and preparing them by extraction of natural raw materials, enzymatic hydrolysis or acid hydrolysis, etc. Reduce harmful bacteria, promote the reproduction of beneficial bacteria, substances that are beneficial to the health of the body, and improve the activity and function of microecological agents (synbiotics)

Method used

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  • Method for preparing efficient probiotic preparation from macrofungi bacterial liquid (mycoplasm)
  • Method for preparing efficient probiotic preparation from macrofungi bacterial liquid (mycoplasm)
  • Method for preparing efficient probiotic preparation from macrofungi bacterial liquid (mycoplasm)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Comparison of the active components of different kinds of macrofungus hyphae fermentation bacteria / bacteria liquid

[0029] 1. Materials

[0030] Large-scale fungal strains: tea mushroom, morel, slide mushroom, Hericium erinaceus, boletus, Bailing mushroom, shiitake mushroom, oyster mushroom, golden mushroom, ganoderma lucidum, Yunzhi, Cordyceps, Poria cocos, bamboo fungus, Pine milk mushroom and other slant strains are preserved by Hunan Minkang Biotechnology Research Institute.

[0031] Liquid medium: 1.5g corn steep liquor, 0.15g ammonium nitrate, 0.4g sodium carboxymethylcellulose, 1.5g glucose, K 2 HPO 4 0.02g, MgSO 4 ·7H 2 O0.05g, distilled water to 100mL, pH6.5.

[0032] Solid medium: solid matrix (40% bran, 30% corn bran, 10% soybean meal, 10% puffed corn, 10% oil bran), nutrient solution (0.5% corn steep liquor, 1% glucose, (NH 4 ) 2 NO 3 0.3%, K 2 HPO 4 0.05%, MgSO 4 ·7H 2 (00.1%, the balance is water, pH6.0-6.5) is 55% of the mass of the...

Embodiment 2

[0043] Example 2 The cultivation test of probiotics by macrofungus bacterium liquid preparation medium

[0044] 1. Materials

[0045] (1) Probiotic strains: Lactacidophilus, Lactobacillus bulgaricus, Bacillus subtilis, B. lichenifofmis, Saccharomycesbayanus.

[0046] (2) Culture medium: the fungal liquid fermentation product (culture liquid and mycelium) that embodiment 1 produces, milk homogenizer homogenate, compound enzyme (0.5~1.5% of cellulase, 1.0%~3.0% of pectinase, protease 1.5-3.0%) enzymolysis, 80 ℃ ultrasonic extraction for 40min, four layers of gauze filtration; take the filtrate, add 2% glucose, 1.5% corn steep liquor, 1% peptone (such as 100mL filtrate, add 2g glucose, 1.5g corn steep liquor, 1g peptone), put it in a Erlenmeyer flask, and sterilize at 121°C for 20min.

[0047] 2. Method

[0048] (1) Secondary inoculation culture: Inoculate 2-5% aerobic probiotic single strain liquid culture composite strains (Bacillus licheniformis: Bacillus subtilis: Saccharo...

Embodiment 3

[0055] Example 3 Determination of antibacterial activity of edible and medicinal fungal mycelia fermentation broth and its cultured probiotics to pathogenic bacteria

[0056] 1. Method: take the liquid cultures of 9 kinds of fungal hyphae in Table 3 respectively, after grinding, enzymatic hydrolysis, ultrasonic treatment and sterilization, secondarily inoculate and cultivate compound probiotics according to the method of Example 2, fungal mycelium The solution and its cultured probiotics were filtered separately (wherein the concentration of the bacterial solution contained about 2% dry bacterial substance, and the bacterial concentration of the probiotic solution was 1.5×10 9 cfu / mL) for spare; use the agar plate culture method to streak inoculate pathogenic bacteria (Escherichia coli C44103, Salmonella C26112, Pasteurella C44-1, Streptococcus 01026), place Oxford cups respectively, and add 50 μL bacterial plasma / bacterial liquid to each cup , cultivated at 37°C for 24h, meas...

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Abstract

The invention discloses a method for preparing an efficient probiotic preparation from macrofungi bacterial liquid (mycoplasm). The method comprises the following steps: preparing a liquid or a solid culture medium from agricultural and sideline products and culturing fungi to obtain a bacterial liquid or a mycoplasm; carrying out physical and biological treatment on the bacterial liquid or the mycoplasm to enable polysaccharide polypeptide substances to be released from cells and adding a nitrogen source and a carbon source to prepare a probiotics culture medium; and then inoculating the probiotics and culturing to obtain the efficient probiotic preparation. The fungi comprise medicinal (edible) fungi of lentinu edodes, agrocybe cylindracea, morchella, pholiota nameko, hericium erinaceus, bolete, pleurotus nebrodensis, pleurotus citrinopileatus, lucid ganoderma, coriolus versicolor, cordyceps taishanensis, poria cocos, bamboo fungus, lactarius deliciosus and the like; and the probiotics is one or more selected from bacillus, saccharomycete, lactobacillus, enterococcus, bifidobacterium and the like. The probiotic preparation prepared by the method disclosed by the invention is rich in active substances, such as polysaccharides and polypeptides, has better health and treatment effects that those of a conventional probiotic preparation and can be used as therapeutic drugs and health foods or feed additives of human and animal gastrointestinal diseases.

Description

technical field [0001] The invention relates to the preparation of probiotic preparations, in particular to a method for preparing high-efficiency probiotic preparations with macrofungus liquid (substance). Background technique [0002] With the endogenous infection and superinfection caused by the long-term use of antibiotics, the emergence of drug-resistant strains, the decline of animal immunity, drug residues and food, a series of problems have aroused widespread concern, making people pay more attention to their health, The awareness of environmental protection and food safety has been continuously strengthened; the requirements for the quality control of the whole process of the breeding industry from breeding to storage, processing, transportation and sales are also increasing. "Pollution-free feed", "ecological feed", "environmentally friendly feed", "organic feed" and "green feed" have attracted much attention. In particular, microbial feed has become one of the cu...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12N1/18C12R1/23C12R1/225C12R1/125C12R1/10C12R1/865
CPCC12N1/20C12N1/14C12N1/16C12N1/18
Inventor 肖兵南高中文刘莹莹
Owner HUNAN MINKANG BIOTECH RES INST
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