Method for propagating dendrobium candidum test-tube plantlets

Abstract

The invention provides a mthod for propagating dendrobium candidum test-tube plantlets, which comprises the following steps: a. adopting dendrobium candidum stem sections or stem section axillary buds as explants, wherein chloroplasts of the stem sections or the stem section axillary buds have gene sequences with the GenBank login number of FJ530946; and b. carrying out the processes of axillary bud survival and growth, cespitose shoot inducement and multiplication, and plantlet formation, wherein the culture conditions include the culture room temperature of 26+ / -2 DEG C, the illumination intensity of 20001x, the pH value between 5.8 and 6.0, the light source of a fluorescent lamp or an LED light source, and the illumination time of 12 h per day. The method of the invention aims at the defects of the existing dendrobe tissue culture method, improves the existing dendrobe tissue culture method, and provides a fast propagation method of the dendrobium candidum germchits. The method can meet the requirements of germchits required by large-scale production in a short time, and has the characteristics of low cost, regular germchit growth, simplicity and easy implementation of a domestication method, good inheritance stability and the like.

Description

technical field The invention relates to a method for propagating test-tube seedlings of dendrobium officinale, belonging to the field of cultivation. Background technique Dendrobium (Dendrobium) is a genus of Orchidaceae, including more than 1,600 species of plants. There are about 76 species in my country, of which Dendrobium candidum is the most precious. Traditional Chinese medicine believes that Dendrobium has the functions of nourishing yin and clearing heat, promoting essence and quenching thirst, improving eyesight and strengthening the body. Modern medicine has found that Dendrobium contains a variety of alkaloids, polysaccharides and amino acids, which can improve immunity, inhibit thrombosis, inhibit tumors and delay aging. Dendrobium is a traditional precious Chinese medicine in my country. Due to the harsh growth conditions, it grows in the crevices or troughs of cliffs and tree trunks in the forest. Endangered. As a traditional precious traditional Chinese m...

AI Technical Summary

Problems solved by technology

However, the above-mentioned artificial light sources generally have disadvantages such as short life, high heat generation, and low luminous efficiency.

However, the utilization rate of the above light sources by plants is only about 50%.

A lot of energy is wasted

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