Polysiphonia urceolata tetraspore seedling formation method

A technique of tetraspores and polytubules, applied in botany equipment and methods, seaweed cultivation, horticulture, etc., can solve problems such as the method of forming seedlings of tetraspores of polytubules that have not been seen yet, and achieve long-term existence and good effect , the effect of short cleaning time

Inactive Publication Date: 2008-04-30
OCEAN UNIV OF CHINA
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Problems solved by technology

The good prospects for development and utilization make the natural resources of Polytubuleum unable to meet the potential development needs, so it is a forward-looking approach to develop methods...

Method used

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Embodiment Construction

[0006] The inventor brought the sporozoites of Polytrichum sporophytes collected from the seashore of Qingdao back to the room during March-April 2007, and selected healthy individuals with mature tetraspores. In the boiled and cooled seawater, scrub with cotton balls until there are no obvious miscellaneous algae and attachments; at this time, there are often a small amount of miscellaneous algae such as diatoms attached to the algae, and they will multiply rapidly once given suitable conditions , affecting the growth of polycystic algae. In order to further clean the algae, immerse the brushed Polytubule sporozoites in clean sea water, and wash them several times with an ultrasonic cell disruptor, generally with a power of 150 watts, and each time no more than 10 seconds. Excessive heat production will cause mechanical damage to the algae or tetrasporangium. Generally, all the algae can be removed after 3-5 times, but clean sea water should be replaced after each cleaning. ...

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Abstract

A method for forming seedlings of polytubule tetraspores, which is characterized in that mature tetraspores are collected from wild populations, and are first washed several times with clean sea water until there are no obvious miscellaneous algae; then ultrasonic cleaning is performed for 3- 5 times, until there is no miscellaneous algae; cut out the part with more sporangia near the end of the algae body and cultivate it at a temperature of 15-20°C, a light intensity of 2000-3000lux, and a light time of 10-12 hours; wait until the spores are released to meet the demand Finally, take away the algae, and continue to cultivate the tetraspores in the water until they germinate into gametophyte algae. Advantages of the present invention: 1. tetrasporocytes germinate into gametophytes of different sexes, which are important materials for related genetics research; 2. ultrasonic cleaning time is short and effective; 3. tetrasporocytes exist in nature for a long time, and biological The quantity is large, which ensures sufficient provenance; 4. The germination rate of tetraspores is high, and offspring algae can be obtained directly without sexual process.

Description

technical field [0001] The invention relates to a method for forming seedlings of polytubule tetraspores. Background technique [0002] Polytubella is a class of red algae belonging to the Rhodophyta Eurythrophyceae Songosteocetaceae. Studies in recent years have shown that Polytubella has antibacterial and fungal, anti-herpes virus and antioxidant activities, and has cytotoxic activity against a variety of cancer cells (such as colon cancer cells, schwannoma cells), so in the development of seaweed drugs It has important development prospects; in addition, Polytubella is also rich in galactose sulfate, phycoerythrin and other substances, which also have important economic value. The good prospects for development and utilization make the natural resources of Polytubuleum unable to meet the potential development needs, so it is a forward-looking approach to develop methods that can be used in artificial cultivation and production, especially the method of seedling acquisiti...

Claims

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Application Information

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IPC IPC(8): A01G33/02
Inventor 汤晓荣仪梅
Owner OCEAN UNIV OF CHINA
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