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Total artificial scytosiphon lomentaria breeding technology

The technology of Hemerocallis algae and the technology is applied in the technical field of artificial seedling raising of Helianthus algae, which can solve the problems of poor seedling attachment and low density, and achieve the effects of simple operation, reduced production cost, and easy popularization and application.

Inactive Publication Date: 2014-06-18
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the defects and drawbacks of the prior art, the purpose of the present invention is to provide a technique for the artificial cultivation of Hemerocallis using spores, which can not only improve the utilization rate of germplasm, but also solve the problems of poor attachment and low density of seedlings.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Day algae growing vegetables were collected from the sea near Changdao Island in Shandong Province in late February of that year. The mature fronds with dark brown algae, gametophytic plaques on the surface and hollow constrictions were selected, and the inner and outer surfaces of the tubular algae were washed with sterile sea water. Place the washed algae in the incubator at 12°C for 3 hours, then put it into the sterile seawater pre-spread with glass slides to release the female and male gametes. After 24 hours, the male and female gametes combine to form zygotes. The zygote slides were cultured in a 250mL Erlenmeyer flask under the conditions of water temperature 16℃, light duration 12 hours, light intensity 18.0-54.0μmol / (m 2 ·S), replace the culture water in full every 3-5 days. After 40 days of culture, the zygote develops into filaments, which is the germplasm of that year.

[0024] The germplasm of the year using Dayalgae was amplified on June 10th. The amplificat...

Embodiment 2

[0027] The method for obtaining the serophyte germplasm was the same as in Example 1. The serophyte germplasm of different regions was collected for many years to establish a serophyte germplasm bank. The healthy germplasm selected from the genebank was expanded on June 1. The expansion was carried out by aerated culture. The other conditions were a temperature of 20°C, a light duration of 12 hours, and a light intensity of 72.0μmol / (m 2 ·S). On September 26, the amplified germplasm was induced under the conditions of 18℃, 10 hours of light, and light intensity of 21.6μmol / (m 2 ·S). Control the nutrient NO in cultivated water 3 — N (10-40mg / mL), PO 4 3— P (20-80 mg / mL), replace the culture water in full every 5-7 days. From the 26th day of induction to October 21st, the single-compartment sporangia of S. dayweed developed and matured, and its characteristics were as follows: the color changed to dark brown, the cells enlarged obviously, and the diameter reached 60-80μm. The ...

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Abstract

The invention relates to a total artificial scytosiphon lomentaria breeding technology. The total artificial scytosiphon lomentaria breeding technology comprises the following steps: (1) performing germplasm preparation, namely preparing germplasm from scytosiphon lomentaria collected in the current year, or preparing the germplasm by the recovery of the germplasm stored in the previous years; (2) performing germplasm amplification, namely amplifying the germplasm of the scytosiphon lomentaria by using an airlift photo-bioreactor; (3) performing germplasm induction, namely inducing the germplasm of the scytosiphon lomentaria to generate a mature unilocular sporangium; (4) performing spore seedling collection, namely stimulating the unilocular sporangium to diffuse spores, and collecting the spores on a net curtain formed by blending vinylon and polyvinyl chloride; (5) performing indoor seedling breeding; (6) performing mariculture, namely performing mariculture in a raft culture manner for 60-80 days to breed commercial scytosiphon lomentaria of which the body length is 60-100cm, wherein 80-120 seedlings exist on a seedling rope which is 1cm long. According to the total artificial scytosiphon lomentaria breeding technology, the scytosiphon lomentaria breeding efficiency can be improved, and the stable yield of commercial seedlings is ensured. The technology has the advantages of high germplasm utilization rate, firm seedling adhesion, low breeding cost, good production benefit and the like, and can be widely applied to large-scale industrial breeding and artificial culture of the scytosiphon lomentaria.

Description

technical field [0001] The invention relates to a seaweed cultivation technology, in particular to a technology for fully artificial cultivation of day lilies. Background technique [0002] Cucumber ( Scytosiphon lomentaria ) belongs to Phaeophyta ( Phaeophyta ), which is a pan-temperate seaweed, is widely distributed in the coastal areas from Liaodong Peninsula in the north to Hailing Island in Guangdong Province in the south in my country. Hemerocallis has an alternate life history of heteromorphic generations. The macroscopic tubular algae is the gametophyte stage, and the microscopic filaments, cushion-like bodies and cushion-like bodies are the sporophyte stage. Filaments, cushions and cushion-like bodies can transform into each other under certain conditions. The key factor affecting the transformation is temperature. High temperature conditions can make filaments form cushion-like bodies and cushion-like bodies, while low temperature conditions make Cushionoids a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G33/00
CPCY02A40/80
Inventor 宫相忠高伟张文健邢永泽张必达
Owner OCEAN UNIV OF CHINA
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