Embedding-dehydrating ultralow temperature storage method for scytosiphon lomentaria mitoplast

A technology of ultra-low temperature preservation and filamentous body, which is applied in the fields of plant preservation, botanical equipment and methods, seaweed cultivation, etc., and can solve the problems of germplasm variation and pollution of Hemerocallis germplasm, and achieve the effect of solving variation and pollution

Active Publication Date: 2014-03-12
OCEAN UNIV OF CHINA
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Problems solved by technology

[0007] The purpose of the present invention is to provide a method for ultra-low temperature preservation of celery filaments by embedding and dehydration, so as to solve the problems of variation and pollutio

Method used

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  • Embedding-dehydrating ultralow temperature storage method for scytosiphon lomentaria mitoplast
  • Embedding-dehydrating ultralow temperature storage method for scytosiphon lomentaria mitoplast
  • Embedding-dehydrating ultralow temperature storage method for scytosiphon lomentaria mitoplast

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Embodiment Construction

[0043] The method of the present invention will be specifically described below in conjunction with the examples.

[0044] A method for embedding and dehydrating ultralow temperature preservation of celery filaments, characterized in that it comprises the following steps:

[0045] (1) Steps for cultivating the filaments of Hemerococcus japonica:

[0046] The mature thallus of Hemerocallis were collected from the natural sea area of ​​Long Island in April 2012. The thallus was repeatedly scrubbed with a sterile cotton swab, then dried in the shade to stimulate and release the female and male gametes. After the female and male gametes were combined, they were cultured for a week to form yellow-brown silk The filaments were collected and expanded and cultured in a light incubator, the culture conditions were (22.0±0.5)°C, and the light intensity was 86.4~97.2μmol / (m 2 s), L:D=14:10, nutrient NO 3 -N is 7g / m 3 ,PO 4 -P is 1.2g / m 3 ; When the filaments are cultivated to brown,...

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Abstract

The invention discloses an embedding-dehydrating ultralow temperature storage method for scytosiphon lomentaria mitoplast. The embedding-dehydrating ultralow temperature storage method comprises the following steps of: collecting mature thallus of the scytosiphon lomentaria, and culturing until brown mitoplast is used as an embedding material; crushing the mitoplast to uniformly mix with sodium alginate liquor containing sucrose and glycerol; dropping NaCl liquor to form rubber balls, and hardening to complete an embedding process; pre-culturing the immobilized rubber balls; placing the rubber balls in a culture dish, placing the culture dish in a large culture dish paved with drying silica gel on bottom, sealing, and immediately placing in an incubator of 21 DEG C for drying and dehydrating under a dark condition; placing the dehydrated rubber balls into a freezing tube, and immediately adding into liquid nitrogen for storage after sealing. The embedding-dehydrating ultralow temperature storage method for scytosiphon lomentaria mitoplast disclosed by the invention effectively solves problems such as variation and pollution in a scytosiphon lomentaria seed storage process; the scytosiphon lomentaria mitoplast has complete growth and development ability and amplification ability after being recovered from freezing, and can form normal sporangium and release spores, so that thallus is developed, a dear apparatus is not needed, and an anti-freezing protective agent with toxic action to the stored material is also not needed to be used. Therefore, popularization and application of the method are facilitated.

Description

technical field [0001] The invention relates to an ultra-low temperature preservation method for embedment and dehydration of celery filaments, and belongs to the technical field of seaweed germplasm preservation. Background technique [0002] Scytosiphon lomentaria (Scytosiphon lomentaria) is a large algae that is widely distributed in the coastal waters from Liaodong Peninsula in the north to Hailing Island in Guangdong Province in the north of my country. Saccharomyces japonica not only has delicious taste and high nutritional value, but also has the characteristics of anti-oxidation, anti-tumor and anti-virus. It is a new type of seaweed with high economic value. Hemerocallis has an alternate life history of heteromorphic generations, consisting of large leafy gametophytic generations and tiny sporophytic generations. The sporophyte generation mainly has three forms: cushion-like, cushion-like and filamentous. The filaments of Hemerocallis are the main object of labora...

Claims

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Application Information

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IPC IPC(8): A01G33/00A01N3/00
Inventor 宫相忠庄琰张文健高伟张必达
Owner OCEAN UNIV OF CHINA
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