Solid-phase preservation method for kelp gametophytes

Abstract

The invention discloses a solid-phase preservation method for kelp gametophytes. The solid-phase preservation method comprises the following steps of scrubbing obtained sporangium kelp blocks; performing germfree treatment on the sporangium kelp blocks; spreading, attaching and germinating kelp spores; separating and inoculating single kelp gametophytes; cultivating the kelp gametophytes in a solid culture medium and performing later-stage preserving. According to the solid-phase preservation method for the kelp gametophytes, the germfree collection and the solid-phase preservation are combined, so that the pollution of germs is inhibited from the sources, the opportunity that the kelp gametophytes pollute the germs is reduced, the germfree treatment flow in the early-stage solid-phase preservation of the kelp gametophytes is greatly simplified, the difficulty in treating the germs on the kelp gametophytes through solid-phase cultivation is alleviated, and the kelp gametophytes with low pollution or even no pollution can be easily obtained; furthermore, the working amount of later replacement of culture liquid during the liquid-phase preservation of the kelp gametophytes is reduced, and a limited preservation space is saved; moreover, the solid-phase preservation method is convenient to popularize and apply.

Description

technical field [0001] The invention relates to a solid-phase preservation method, in particular to a solid-phase preservation method of kelp gametophytes, which belongs to the technical field of marine biology. Background technique [0002] Kelp is an important economic seaweed in China. In the 1970s, the collection and cloning technology of kelp gametophytes was established, which laid the foundation for the germplasm protection of kelp. Gametophytes collected by traditional methods are generally separated into test tubes and sealed with cotton plugs for storage. Usually, the culture medium is updated once a month, which is a heavy workload and cumbersome operation, which increases the chances of bacterial contamination and germplasm loss and mixing; Shandong Dongfang in 2005 Marine Technology Co., Ltd. and Yantai University jointly developed the solid-phase preservation technology of kelp gametophytes, but due to the extension of the preservation time of the original game...

AI Technical Summary

Problems solved by technology

Gametophytes collected by traditional methods are generally separated into test tubes and sealed with cotton plugs for preservation. Usually, the culture medium is updated once a month. The workload is heavy and the operation is cumbersome, which increases the chances of bacterial contamination and germplasm loss and mixing; Shandong Dongfang in 2005 Marine Science and Technology Co., Ltd. and Yantai University jointly developed the solid-phase preservation technology of kelp gametophytes, but due to the extension of the preservation time of the original gametophyte clones in the laboratory, changes in the external environment and sea water quality, and the open operation of liquid-phase culture and many other factors , by the end of 2008, most of the gametophytic clones of Laminaria preserved by the end of 2008 were contaminated by bacteria, some even had fungi, and the composition of the contaminated bacteria was complex, not a single kind of bacteria. Gametocyte cloning is often difficult to achieve a sterile state, and it is easy to grow bacteria after inoculation, resulting in the failure of solid-phase culture, which affects the popularization and application of solid-phase preservation technology

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