Culture method of helminthosporium sporangium spores

A culturing method and technology for Helminthosporium, which are applied in the field of microorganisms, can solve the problems of increasing drug-resistant biotypes of barnyardgrass, threats to the sustainable development of environment and ecological security, and pollution of farmland and water environment, and achieve spore culture and production. The method is simple and easy to implement, the design is reasonable, and the cost is low.

Inactive Publication Date: 2010-11-17
CHINA NAT RICE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although chemical herbicides can quickly control barnyardgrass in paddy fields, long-term large-scale use also brings many negative effects, such as the increase of resistant biotypes of barnyardgrass, and the pollution of farmland and water environment in some areas. development poses a potential threat, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1) Preparation of inoculum

[0022] Transfer the starting strain Helminthospora graminearum stored on a slope at 4°C to fresh PDA medium, activate and cultivate it under dark conditions at 27°C for 8 days, take a 6.5mm diameter bacterial block, inoculate it in PDB medium, and inoculate it in PDB medium. Shake culture at 27°C and 100r / min for 2 days, then inoculate 8mL of the bacterial suspension in the logarithmic growth phase into 100mL of fresh PDB, shake at 27°C and 100r / min for 2 days, spare;

[0023] 2) Preparation of Helminthospora graminearum spore production medium

[0024] Use perlite as the substrate matrix, add wheat flour with 4% volume of perlite, soybean meal powder with 0.5% volume of perlite, and MgSO with 0.1% volume of perlite 4 , Perlite volume 0.2% Na 3 PO 4 50% of the volume of perlite and tap water; after mixing evenly, the culture medium was sterilized at 121°C for 20 minutes;

[0025] 3) Cultivation of Helminthospora graminearum spores

[0...

Embodiment 2

[0030] 1) Preparation of inoculum

[0031] Transfer the starting strain H. graminearum stored on a slant at 4°C to fresh PDA medium, activate and cultivate it under dark conditions at 28°C for 7 days, take a 5.5mm diameter bacterial block, inoculate it in PDB medium, and inoculate it in PDB medium. Shake culture at 28°C and 150r / min for 3 days, then inoculate 10mL of the bacterial suspension in the logarithmic growth phase into 100mL fresh PDB, shake at 28°C and 150r / min for 3 days, spare;

[0032] 2) Preparation of Helminthospora graminearum spore production medium

[0033] Use perlite as the substrate matrix, add rice flour with 4% volume of perlite, soybean meal powder with 1% volume of perlite, and MgSO with 0.1% volume of perlite 4 , Perlite volume 0.2% Na 3 PO 4 and perlite volume 40% tap water; after mixing evenly, the culture medium was sterilized at 121°C for 20 minutes;

[0034] 3) Cultivation of Helminthospora graminearum spores

[0035] Get step 2) the H. gra...

Embodiment 3

[0037] 1) Preparation of inoculum

[0038] Transfer the starting strain H. graminearum stored on a slant at 4°C to fresh PDA medium, activate and cultivate it under dark conditions at 28°C for 7 days, take a 5.5mm diameter bacterial block, inoculate it in PDB medium, and inoculate it in PDB medium. Shake culture at 28°C and 150r / min for 3 days, then inoculate 10mL of the bacterial suspension in the logarithmic growth phase into 100mL fresh PDB, shake at 28°C and 150r / min for 3 days, spare;

[0039] 2) Preparation of Helminthospora graminearum spore production medium

[0040] Using powdered wheat straw as the substrate matrix, add oat flour with 6% volume of wheat straw, soybean protein powder with 0.5% volume of wheat straw, and MgSO with 0.2% volume of wheat straw 4 , 0.1% Na by volume of wheat straw 3 PO 4 30% of the volume of wheat straw and tap water; after mixing evenly, the culture medium was sterilized at 121°C for 20 minutes;

[0041] 3) Cultivation of Helminthosp...

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Abstract

The invention relates to a culture method of helminthosporium sporangium spores, belonging to the technical field of microorganisms. The culture method of helminthosporium sporangium spores comprises the following steps of: (1) preparing an inoculum; (2) preparing a production culture medium of helminthosporium sporangium spores; and (3) culturing the helminthosporium sporangium spores. The culture method of the helminthosporium sporangium spores has reasonable design, the production culture medium in the invention has simple composition and low cost, the spore culture and production method is simple and practical, can reach the yield of 0.0213 + / - 0.00145 billion spores on each gram of substrate medium and is possible to develop helminthosporium sporangium spore preparations.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a method for cultivating Helminthospora graminearum spores. Background technique [0002] Barnyardgrass is a vicious weed in paddy fields. If it is not controlled, it will seriously affect the yield of rice. At present, it is mainly controlled by chemical herbicides. Although chemical herbicides can quickly control barnyardgrass in paddy fields, long-term large-scale use also brings many negative effects, such as the increase of resistant biotypes of barnyardgrass, and the pollution of farmland and water environment in some areas. development poses a potential threat, etc. Microbial herbicides are biological agents developed using biological living organisms or biologically active substances. They have the advantages of no pollution to the environment, no residues, and are not easy to cause drug resistance in crops. Therefore, they are good supplements and sub...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N3/00C12R1/645
Inventor 段桂芳杨爽张建萍余柳青
Owner CHINA NAT RICE RES INST
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