Application of litsea cubeba essential oil in controlling pepper phytophthora blight

A technology of pepper blight and litsea cubeba, applied in the field of application of litsea cubeba essential oil to control pepper blight, can solve problems such as difficult production and cultivation, human health hazards, and threats to food safety, so as to inhibit the development and spread of diseases, The effect of good protective effect and good therapeutic effect

Active Publication Date: 2017-10-10
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, in order to reduce the losses caused by pepper blight, a lot of research has been done on the prevention and treatment of pepper blight in the aspects of genetic breeding, farming measures, and chemical control at home and abroad, but no disease-resistant germplasm materials have been found so far; The management measures are more complicated, and it is difficult to implement large-scale production and cultivation; the most...

Method used

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  • Application of litsea cubeba essential oil in controlling pepper phytophthora blight
  • Application of litsea cubeba essential oil in controlling pepper phytophthora blight
  • Application of litsea cubeba essential oil in controlling pepper phytophthora blight

Examples

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Effect test

Embodiment 1

[0019] Dissolving the litsea cubeba essential oil in acetone, adding Tween-60, and finally diluting and mixing with sterile water to obtain the medicinal solution of the required concentration of the litsea cubeba essential oil. The concentration of Litsea Cubeba essential oil, 0.2 g acetone, and 1 g Tween-60 in each 1 L of medicinal solution.

[0020] The preparation method of the above-mentioned litsea cubeba essential oil comprises the following steps: drying, grinding and sieving the litsea cubeba fruit to obtain litsea cubeba powder; 2 extraction to obtain litsea cubeba essential oil; among them, supercritical CO 2 Extraction conditions: extraction temperature 45°C, extraction time 60 minutes, extraction pressure 2.5 MPa, CO 2 Flow rate 1.5 mL / min.

Embodiment 2

[0022] Dissolving the litsea cubeba essential oil in methanol, adding Tween-80, finally diluting and mixing with sterile water to obtain the medicinal solution of the required concentration of the litsea cubeba essential oil. The concentrations of Litsea Cubeba essential oil, 0.3 g methanol, and 1.5 g Tween-80 were contained in each 1 L of medicinal solution.

[0023] The preparation method of the above-mentioned litsea cubeba essential oil comprises the following steps: drying, grinding and sieving the litsea cubeba fruit to obtain litsea cubeba powder; 2 extraction to obtain litsea cubeba essential oil; among them, supercritical CO 2 Extraction conditions: extraction temperature 40°C, extraction time 80 minutes, extraction pressure 2 MPa, CO 2 The flow rate is 2 mL / min.

Embodiment 3

[0025] Dissolving the litsea cubeba essential oil in ethanol, adding Tween-60, and finally diluting and mixing with sterile water to obtain the medicinal solution of the required concentration of the litsea cubeba essential oil. The concentration of Litsea Cubeba essential oil, 0.4 g of ethanol, and 2 g of Tween-60 in each 1 L of liquid medicine.

[0026] The preparation method of the above-mentioned litsea cubeba essential oil comprises the following steps: drying, grinding and sieving the litsea cubeba fruit to obtain litsea cubeba powder; 2 extraction to obtain litsea cubeba essential oil; among them, supercritical CO 2 Extraction conditions: extraction temperature 50°C, extraction time 40 minutes, extraction pressure 3 MPa, CO 2 The flow rate is 1.2 mL / min.

[0027] The liquid medicine prepared in Examples 1-3 is applied to the crops by spraying the whole plant.

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Abstract

The invention discloses an application of litsea cubeba essential oil in controlling pepper phytophthora blight. The litsea cubeba essential oil is dissolved in an organic solvent, and then a Tween emulsifying agent is added and lastly the mixture is diluted with sterile water and is uniformly mixed, so that the liquor in the required litsea cubeba essential oil concentration is acquired; the liquor is applied in the manner of spraying or root irrigation; the litsea cubeba essential oil is prepared from the following steps: drying, grinding and screening the litsea cubeba fruits, thereby acquiring the litsea cubeba powder, and then adopting supercritical CO2 extraction for the litsea cubeba powder, thereby acquiring the litsea cubeba essential oil. The liquor prepared from the litsea cubeba essential oil according to the invention is an efficient inhibitor for pepper phytophthora blight, has an excellent field control effect for pepper phytophthora blight and can be used for comprehensively treating pepper phytophthora blight. The litsea cubeba essential oil can be used for preventing and controlling in different stages of the diseases. The drug, especially applied before the forming of sporangia, can effectively restrain the secondary infection of the pathogenic bacteria and the development and propagation of the diseases.

Description

technical field [0001] The invention belongs to the technical field of agricultural prevention and control, and in particular relates to the application of litsea cubeba essential oil in controlling capsicum blight. Background technique [0002] Litsea cubeba essential oil is the volatile oil extracted from the fresh fruit of Litsea cubeba Pers, a deciduous shrub of the family Zingiberaceae. It is an edible natural spice that is allowed to be used according to GB2760-2011, and it is the most exported plant essential oil of a single species in my country. "Dictionary of Chinese Medicine" records that it has the effects of expelling wind and cold, regulating qi and killing insects, detoxifying and relieving pain, and is one of the commonly used folk medicines. Initially, Zhou Yong and others found that litsea cubeba essential oil is a broad-spectrum antifungal drug through experimental research, and confirmed that it has a bactericidal effect. Subsequently, researches on the ...

Claims

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Application Information

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IPC IPC(8): A01N65/24A01P3/00
CPCA01N65/00A01N65/24A01N2300/00
Inventor 周琳高飞赵特王轶楠王海娇刘向阳那日松王合中
Owner HENAN AGRICULTURAL UNIVERSITY
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