Method for cloning and culturing seaweed gametophytes

A cultivation method and gametophyte technology are applied in the field of cultivation of kelp gametophyte clones, which can solve problems such as unfavorable long-term preservation, loss of germplasm resources, difficulty in long-term preservation, etc., and achieve the effects of reducing cultivation costs, reducing workload, and maintaining stable traits.

Inactive Publication Date: 2012-11-14
SHANDONG ORIENTAL OCEAN SCI TECH
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Problems solved by technology

However, due to the incomplete disinfection of kelp and ambient air in this method, bacteria, fungi and algae pollution are very prone to occur, thereby affecting the growth and survival rate of gametophytes, which is not conduci...

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  • Method for cloning and culturing seaweed gametophytes
  • Method for cloning and culturing seaweed gametophytes
  • Method for cloning and culturing seaweed gametophytes

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Embodiment Construction

[0019] Below in conjunction with accompanying drawing and embodiment describe in detail:

[0020] A kind of kelp gametophyte cloning cultivation method of the present invention is realized by the following methods:

[0021] 1. Clipping the kelp block: clipping the sporangia part of mature kelp that has excellent properties, no disease and rot, and has not been released, that is, cutting the kelp block with mature sporangia. The cultured kelp is annual, and the sporangia of cultured kelp generally appear from May to August every year; the wild kelp is biennial, and the sporangia of wild kelp generally appear from October to November every year.

[0022] 2. Pretreatment of the kelp block: pretreat the kelp block with mature sporangia, that is, use sterilized absorbent cotton to soak the boiled and sterilized seawater cooled to 10°C, and carry out the treatment on the kelp block with mature sporangia. Scrub fully to remove mucus, miscellaneous algae and other attachments on the ...

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Abstract

The invention discloses a method for cloning and culturing seaweed gametophytes, which comprises the following steps of: cutting seed seaweed pieces with mature sporangia; using sterilized absorbent cotton to dip the boiled seawater for cleaning the seed seaweed pieces; respectively soaking the pretreated seed seaweed pieces with sterilized double-resistant seawater for 30 minutes, sterilized seawater solution with the potassium iodide concentration being 1.5% for 10 minutes, and sterilized seawater for 20 minutes; carrying out free spore diffusion and filtration in a serum bottle; and pouring the filtered spore water into a vertical staining jar which is provided with a glass slide, after the free spores are attached to the glass slide to form embryo spores, pouring the spore water out, filling sterilized double-resistant seawater, using a micropipette to separate single male and female gametophytes to the serum bottle, then using sterilized double-resistant seawater to culture, and thus obtaining the unicellular seaweed gametophyte clone group. The seaweed gametophyte which is cultured by the method has stable clone characteristics and high survival rate, can be effectively prevented from being contaminated, and is suitable for long-term storage.

Description

technical field [0001] The invention relates to a method for cultivating kelp gametophyte clones, in particular to a method for aseptically cultivating kelp gametophyte clones. Background technique [0002] Kelp, scientific name Laminaria japonica Aresch, is a kind of brown algae. It grows on the rocks on the seabed. It is shaped like a belt and contains a lot of iodine. The algal body is brown, long strip-shaped, leathery, generally 2-6 meters long and 20-30 cm wide. The algae are clearly divided into holdfasts, stalks and leaves. The holder is rhizoid-shaped, the stalk is thick and short cylindrical, and the upper part of the stalk is a wide and long ribbon-shaped leaf. Kelp has extremely high breeding value, but the breeding process of fine breeding varieties is complicated and lengthy. Laminaria gametophyte can carry out vegetative growth to form asexual reproduction line (clone) when isolated and cultured separately, its heredity is pure, can be preserved for a long ...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G33/00
CPCY02A40/80
Inventor 孙娟李霞赵楠张壮志李晓捷钱冠兰罗世菊李言赛珊王娜宋少峰
Owner SHANDONG ORIENTAL OCEAN SCI TECH
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