Method for producing gynoecial loaches

Abstract

The invention discloses a method for producing gynoecial loaches. The method comprises the following steps: (1) artificial propagation and artificial insemination; (2) sex reversal: reversing most XX male fishes in fries into XX physiological male fishes by taking artemia larvae soaked into a male hormone solution as a bait, remaining XY male fishes unchanged, and removing XX female fishes till male fishes and female fishes can be distinguished; (3) test mating: feeding the XX physiological male fishes and the XY male fishes to a sexually mature state, mating with the XX female fishes respectively, and remaining a parent of which all offspring are XX female fishes, namely, the XX physiological male fishes; (4) reproduction: mating the XX physiological male fishes and the XY male fishes to produce 100 percent XX female fries, and carrying out sex-reversing on a part of the female fries into XX physiological male fishes according to the step (2); (5) establishment of a population: mating the XX female fishes and the XX physiological male fishes to produce 100 percent XX female fishes, and repeating the steps (4) and (5) to continuously produce gynoecial loaches on a large scale.

Description

technical field [0001] The invention relates to the artificial sex control technology of fish, which belongs to a method of physiological genetic reproduction of fish, and is the technical field of artificial sex reversal, test cross screening, group expansion and other technologies to control the breeding of single-sex fish, and specifically relates to a Method for producing whole female loach. Background technique [0002] Loach is an important fish in freshwater culture. Under the same breeding conditions, female loaches grew faster and gained more weight than compatriot males. Breeding all-female loach will significantly increase the breeding yield and economic benefits. [0003] There are several methods for producing parthenogenetic females: artificial selection, direct estrogen-induced sex reversal, gynogenesis, and sex-specific DNA probe technology. The artificial selection method is not suitable for large-scale production of single-sex female fish because of its...

AI Technical Summary

Problems solved by technology

The artificial selection method is not suitable for large-scale production of single-sex female fish due to its high labor intensity and inability to accurately distinguish male and female fish

The method of direct estrogen-induced sex reversal can make male fish sex-reversed into female fish on a large scale, but this method cannot achieve the goal of all females, and there is a risk of residual sex hormones, which is not conducive to safe production, and has long been banned by many countries

At present, the method of using gynogenesis to produce all-female fish has been applied to aquaculture production, but the biggest difficulty of this method is the low frequency of diploid occurrence, low survival rate of larvae and juvenile fish, large individual differences, and the ability to reach sexual maturity. Not many individuals

Images