Production method of sex-reversed male loach

Abstract

The invention provides a production method of sex-reversed male loach. The method comprises the first step of conducting incubation; the second step of conducting temperature regulatory reversion, wherein after loach fry is hatched and out of membrane, and before genital ridge of the loach fry is formed, the water temperature is gradually raised to 27-32 DEG C to cultivate the loach fry to juvenile loach; the third step of conducting male hormone regulatory reversion, wherein after the loach fry is hatched and out of membrane, artemia larva and pellet feed which are treated by a male hormone solution are mixed and continuously thrown through a gradual-transition mode; the fourth step of conducting normal feeding, wherein the water temperature is adjusted to a normal water temperature, then the pellet feed which is not treated by the male hormone solution is thrown, and then XX male loach is removed when the loach is fed to the state that the male loach and the female loach can be distinguished; the fifth step of conducting test cross, wherein XX physiological male loach and XY male loach are fed to be sexually mature, then the XX physiological male loach and XY male loach are mated with XX male loach separately, and then male parent of which the filial generation is 100%XX male loach is screened out, namely, XX sex-reversed male loach. Through a joint effect of the temperature and male hormone, regulatory reversion is conducted, so that the survival rate and the sex reversal rate of the loach fry can reach an optimal state simultaneously, and further the production method of the sex-reversed male loach can be used for conducting scale production of full-female loach.

Description

technical field [0001] The invention relates to fish artificial sex control technology, belongs to the technical field of fish genetics and breeding, in particular to a production method of pseudo-male loach, which is applied to the large-scale production of all female loach seedlings. Background technique [0002] Loach meat is delicious, has high nutritional value, and has a broad market prospect. It is an important fish for freshwater aquaculture. Under the same breeding conditions, female loaches grew faster and gained more weight than compatriot males. Breeding all-female loach will significantly increase the breeding yield and economic benefits. [0003] In actual production and application, XX♀ common female fish and XX♂ pseudo-male fish (genetically female, physiologically male) can be artificially inseminated to obtain parthenogenetic female fish on a large scale. However, there are several ways to obtain XX♂ pseudo-male fish: such as temperature control, direct i...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the invention is to overcome the problem that the survival rate and sexual reversal rate of the existing loach pseudo-male production cannot reach the optimal state at the same time

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