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Artificially induced gynogenesis method for Pseudobagrus ussuriensis

A technology of artificial induction and gynogenesis, applied in fish farming, application, climate change adaptation, etc., can solve problems such as no reports of gynogenesis, achieve broad prospects for industrialized breeding, increase production, and simple and easy irradiation equipment line effect

Active Publication Date: 2013-11-27
HUAIYIN TEACHERS COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there have been no reports of gynogenesis in this fish at home and abroad

Method used

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  • Artificially induced gynogenesis method for Pseudobagrus ussuriensis
  • Artificially induced gynogenesis method for Pseudobagrus ussuriensis

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Embodiment Construction

[0021] Attached below figure 1 Further detailing the method for artificially inducing gynogenesis of Ussuri catfish of the present invention, specifically implement according to the following steps:

[0022] 1. Induced labor of broodstock: Select male and female Ussuri breams with strong physique, undamaged skin, plump and soft abdomen, and perform artificial sexual maturation to induce labor.

[0023] 2. Kill the male fish and take the testis: dissect the male fish and take the testis, cut it into pieces with scissors in a mortar, grind and homogenate, dilute the semen to the sperm preservation solution at a volume ratio of 1:5, and filter it with gauze silk to 1.0-1.8 mm thickness is evenly laid into a 90mm diameter petri dish;

[0024] The sperm preservation solution is prepared as follows: each 500ml solution contains 2.5-4.0g of NaCl, 1.0-1.6g of KCl, and 1.0-1.6g of CaCl 2 0.1~0.2g, MgSO 4 .7H 2 O 0.1~0.2g, NaHCO 3 0.2~0.4g, preservative 0.1~0.3g.

[0025] 3. Sp...

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Abstract

The invention discloses an artificially induced gynogenesis method for Pseudobagrus ussuriensis, comprising the steps of heredity inactivation of sperms, cold shock treatment of germ cells, and hatching; the best time for inactivating the sperms of the Pseudobagrus ussuriensis is found out by regulating the irradiation distance and the irradiation time of ultraviolet; the cold shocks with different time spans are carried out at different time starts after fertilization, the treatment time for a highest survival rate is determined to be the proper time span of the cold shock, and thus the optimal condition suitable for artificially induced gynogenesis of the Pseudobagrus ussuriensis is screened out. According to the technical route of milter sex reversal-artificial gynogenesis-super-milter cultivating-complete milter cultivating, the invention provides a technological base for cultivating the super-milter of the Pseudobagrus ussuriensis and cultivating the monotropic complete milter, so that the cultivation scale is exoanded and the yield is greatly increased, so that the income of farmers is greatly increased, and therefore, the method has a broad industrialized cultivation prospect.

Description

technical field [0001] The invention relates to the technical field of fish genetics and breeding, in particular to a method for artificially inducing gynogenesis of Ussuri breamus. Background technique [0002] Fish have various methods such as sexual reproduction, gynogenesis, and hybrid reproduction to reproduce offspring and continue the population. Gynogenesis refers to the sexual reproduction mode in which eggs must be stimulated by sperm to produce individuals with only maternal genetic material. The common characteristic of fishes that engage in natural gynogenesis is that the number of chromosomes of mature eggs does not halve, and remains the same as that of somatic cells. When the sperm of hermaphrodites enters the egg, it only activates the egg. The sperm nucleus is generally in a compact state, does not undergo prokaryosis, and does not fuse with the female pronucleus. The offspring produced are similar to the mother, and the genotype is the same as the mother ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 潘正军周凤建强晓刚丁怀宇常国亮
Owner HUAIYIN TEACHERS COLLEGE
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