Oncorhynchus mykiss all-female triploid large-scale production method

A technology of triploid and rainbow trout, applied in the direction of climate change adaptation, application, fish farming, etc., can solve the problem of inability to realize the mass production of all-female triploid of rainbow trout, and achieve the effect of breaking technical barriers

Active Publication Date: 2022-05-27
HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a large-scale seed production method of rainbow trout all-female triploid, to solve the problem that the prior art cannot realize the mass production of rainbow trout all-female triploid

Method used

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  • Oncorhynchus mykiss all-female triploid large-scale production method
  • Oncorhynchus mykiss all-female triploid large-scale production method
  • Oncorhynchus mykiss all-female triploid large-scale production method

Examples

Experimental program
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Effect test

Embodiment 1

[0035] 1: Creation of all-female germplasm of rainbow trout and preparation of pseudo-male fish

[0036] (1) Creation of all-female germplasm of rainbow trout:

[0037] For parental selection, the fast-growing female rainbow trout was selected as the female parent, and the male golden trout was selected as the male parent. Ultraviolet inactivation of genetic material of golden trout sperm: Dilute golden trout sperm into sperm culture medium at a dilution ratio of 1:100, put 1 ml of the diluted semen in a petri dish with a diameter of 9 cm, and then irradiate it under a UV lamp with an irradiation intensity of 6 μW / cm 2 , irradiate for 65s, collect the irradiated semen to reach 100ml, and store it in a 4°C refrigerator until artificial insemination;

[0038] Artificial insemination: mix UV-inactivated sperm with rainbow trout eggs, add water immediately, activate sperm, and record the time;

[0039] Pressure treatment to inhibit the second meiosis and induce gynogenesis: put ...

Embodiment 2

[0067] Except for adjusting the following steps in this embodiment, the remaining steps are the same as those in Embodiment 1. The adjustment steps are:

[0068] The insemination water temperature was adjusted to 12°C, the pressure value of placing the fertilized eggs in the press was adjusted to 8000PSI, the pressure treatment time was adjusted to 15 minutes, the temperature in the press was adjusted to 11°C, and the temperature of the seed production workshop was adjusted to 12°C ; Adjust the water temperature of the incubator to 12°C.

[0069] Statistics show that the female rate of this example is 99%, the triploid rate is 98%, and the seed production survival rate is 80%.

Embodiment 3

[0071] Except for adjusting the following steps in this embodiment, the remaining steps are the same as those in Embodiment 1. The adjustment steps are:

[0072] Adjust the optimal age of the sex-reversed male (pseudo-male) rainbow trout parents to 3 years old, adjust the pH value of the final solution in the preparation step of the sperm culture solution to 9.2, adjust the insemination water temperature to 11°C, and put the fertilized eggs in The pressure value in the pressure vessel was adjusted to 8500 PSI, the pressure treatment time was adjusted to 5 minutes, the temperature in the pressure vessel was adjusted to 11°C, and the temperature of the seed production workshop was adjusted to 12°C; the water temperature of the incubator was adjusted to 12°C.

[0073] Statistics show that the female rate of this example is 99%, the triploid rate is 97%, and the seed production survival rate is 80%.

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Abstract

The invention discloses a rainbow trout all-female triploid large-scale seed production method, and belongs to the technical field of fish sex regulation and control and ploidy breeding. According to the method, a gynogenesis technology based on body color marking is developed, all-female germplasm is created in batches, then the all-female germplasm is induced into sex reversal male fish (pseudo-male fish) in batches through a sex control technology, all-female fertilized eggs are obtained through hybridization of the pseudo-male fish and common female fish, and the sex reversal male fish is obtained through pressure treatment. The pressure and the placing time of the fertilized eggs in the pressure device are limited, and finally the purpose of batch production of rainbow trout all-female triploids is achieved. The method effectively solves the problem of triploid offspring seed supply in the rainbow trout breeding industry, and provides a solution for localization substitution of imported triploid offspring seeds.

Description

technical field [0001] The invention relates to the technical field of fish sex regulation and ploidy breeding, in particular to a large-scale seed production method of full female triploid of rainbow trout. Background technique [0002] Fish ploidy breeding (genome doubling) is one of the key research areas of genetic breeding, and production costs can be effectively reduced through genetic improvement. Triploid female fish has become a research focus of domestic and foreign aquatic scientists because of its advantages of fast growth, increased meat weight, improved quality and easy feeding and management. Since the 1980s, triploid fish has Considerable progress has been made in the artificial induction of fish, because the sterility of triploid female fish is of great significance to control the over-breeding of farmed fish and protect natural germplasm resources. Therefore, the international research work on polyploidy induction is progressing rapidly. [0003] Triploid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10A01K61/17
CPCA01K61/10A01K61/17Y02A40/81A01K67/02
Inventor 徐革锋黄天晴刘恩慧谷伟王炳谦王高超
Owner HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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