Method for artificially inducing sex reversal of female siniperca chuatsi

Abstract

The invention discloses a method for artificially inducing sex reversal of female siniperca chuatsi. The method comprises the following steps of (1) breeding siniperca chuatsi parents; (2) conductingartificial spawning induction of the siniperca chuatsi; (3) obtaining heterologous sperms of siniperca scherzeri; (4) conducting genetic inactivation of the heterologous sperms of the siniperca scherzeri; (5) obtaining mature eggs of the siniperca chuatsi; (6) performing artificial gynogenesis on the siniperca chuatsi to obtain female individuals; and (7) artificially inducing sexual transformation of gynogenesis siniperca chuatsi. According to the method, the siniperca chuatsi can be subjected to artificial gynogenesis induction to obtain all-female siniperca chuatsi, then the all-female siniperca chuatsi is induced by artificial male hormone methyl testosterone (MT) to generate pseudo-male fishes with XX genotypes, sperms of the pseudo-male fishes have the same fertilization capacity ascommon male fishes, and a large number of all-female seedlings can be obtained as common female fish parents by the pseudo-male fishes and common female fishes; and the culture yield and the commercial value are greatly improved.

Description

technical field [0001] The invention belongs to the field of fish genetics and breeding, and in particular relates to a method for artificially inducing sex reversal of female cockroaches. Background technique [0002] Commonly known as sweet-scented osmanthus fish, it belongs to the family Perciformes and Siniperidae. It is the largest and fastest-growing fish in the genus Siniperca. It is a rare edible fish native to major rivers and lakes in China. The research object "Mandarin mandarin fish" mentioned in this project refers to "Qiaozui Siniperca mandarin fish". According to statistics, the annual output of mandarin fish farming in the country has reached more than 500,000 tons, with a direct annual output value of about 30 billion yuan, occupying a very important position in the breeding of famous freshwater fish. However, 90% of the mandarin fish seedling breeding units in the country do not pay attention to the breeding and conservation of mandarin fish broodstock. T...

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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for artificially inducing the sexual reversal of female cockroaches in order to solve the problems of germplasm degradation, slow growth, poor disease resistance, and high deformity rate during the breeding and breeding of mandarin fish seedlings in the prior art. Artificial gynogenetic induction of Siniperca sinensis is carried out first, and the offspring female fish are induced by artificial androgen to produce "pseudo male fish" with XX genotype, and the sperm of pseudo male fish has the same fertilization ability as ordinary male fish, which can realize Large-scale normal breeding of all female offspring as breeding seedlings solves the above technical problems

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