Micro-organism preparation for preventing and curing streptococcicosis of Tilapia mossambica as well as preparation method and application thereof

A technology for microbial preparations and streptococcal disease, applied in applications, chemical instruments and methods, biological water/sewage treatment, etc., can solve rare problems, achieve the effects of small usage, improve immunity, and reduce economic losses

Inactive Publication Date: 2012-07-18
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the current microbial preparations are mainly aimed at improving water quality and enhancing body digestion, and specialized antibacterial and disease-preventing microbial preparations are still rare. Microbial preparations for the prevention and treatment of tilapia streptococcosis are very necessary

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Mix strain A (Nocardia rubrum ATCC 11653) and strain B (Nocardia corallicolor ACCC 40100), and the ratio is based on the dry weight of bacterial cells. Strain A: strain B is 1:1;

[0036] (2) Medium:

[0037] General medium for obtaining large biomass: use inorganic nitrogen source and organic carbon source as nutrients; general medium formula is: potato 200g / L, glucose 20g / L, soluble starch 25g / L, ammonium sulfate 5g / L, Dipotassium hydrogen phosphate 0.85g / L; the specific preparation method is as follows: first wash and peel the potatoes, then weigh 200g and cut them into small pieces, add water to boil until it can be pierced by a glass rod, filter with four layers of gauze, and the filtrate Add glucose and soluble starch to the mixture, continue heating and stirring to mix well, then add other ingredients after cooling down, and make up water to 1000 ml, adjust pH to 7.5;

[0038] 3) Strain fermentation preparation:

[0039] a) Shaker culture: put strain A and...

Embodiment 2

[0045] (1) Mix strain A (Nocardia rubrum ATCC 11653) and strain B (Nocardia corallicolor ACCC 40100), and the ratio is based on the dry weight of bacterial cells. Strain A: strain B is 1:2;

[0046] (2) Medium:

[0047] General medium for obtaining large biomass: use inorganic nitrogen source and organic carbon source as nutrients; general medium formula is: potato 200g / L, glucose 20g / L, soluble starch 25g / L, ammonium sulfate 5g / L, Dipotassium hydrogen phosphate 0.85g / L; the specific preparation method is as follows: first wash and peel the potatoes, then weigh 200g and cut them into small pieces, add water to boil until it can be pierced by a glass rod, filter with four layers of gauze, and the filtrate Add glucose and soluble starch to the mixture, continue heating and stirring to mix well, then add other ingredients after cooling down, and make up water to 1000 ml, adjust pH to 7.5;

[0048] 3) Strain fermentation preparation:

[0049] a) Shaker culture: put strain A and...

Embodiment 3

[0055] (1) Mix strain A (Nocardia rubrum ATCC 11653) and strain B (Nocardia corallicolor ACCC 40100), and the ratio is based on the dry weight of bacterial cells. Strain A: strain B is 2:1;

[0056] (2) Medium:

[0057] General medium for obtaining large biomass: use inorganic nitrogen source and organic carbon source as nutrients; general medium formula is: potato 200g / L, glucose 20g / L, soluble starch 25g / L, ammonium sulfate 5g / L, Dipotassium hydrogen phosphate 0.85g / L; the specific preparation method is as follows: first wash and peel the potatoes, then weigh 200g and cut them into small pieces, add water to boil until it can be pierced by a glass rod, filter with four layers of gauze, and the filtrate Add glucose and soluble starch to the mixture, continue heating and stirring to mix well, then add other ingredients after cooling down, and make up water to 1000 ml, adjust pH to 7.5;

[0058] (3) Strain fermentation preparation:

[0059] a) Shaker culture: put strain A an...

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Abstract

The invention relates to a micro-organism preparation for preventing and curing streptococcicosis of Tilapia mossambica as well as a preparation method and an application thereof. The micro-organism preparation method for preventing and curing streptococcicosis of Tilapia mossambica comprises steps of compounding mixed vaccine with red Nocardia ATCC11653 and coral Nocardia ACCC40100, fermenting and cultivating the mixed vaccine with fermentation medium which is compounded of nitrogen source, carbon source and inorganic salt, and processing resultants into solid powder after the fermentation and cultivation. The micro-organism preparation for preventing and curing streptococcicosis of Tilapia mossambica, which provided by the invention, can reduce the content of ammonia nitrogen and nitrate nitrogen in the Tilapia mossambica breeding water body, antagonize the streptococcicosis of the Tilapia mossambica, reduce the accumulation of harmful substances of the middle and later breeding periods, enhance the immunity of the Tilapia mossambica,, achieve the aim of preventing and curing streptococcicosis of Tilapia mossambica, reduce the economic loss caused by diseases, and ensure the breeding output and quality of the Tilapia mossambica. The micro-organism preparation provided by the invention has advantages of convenience in use, small amount of usage and stable effect after use.

Description

technical field [0001] The invention relates to a microbial preparation for preventing and treating tilapia streptococcosis, a preparation method and application thereof, and belongs to the technical field of aquaculture. Background technique [0002] Tilapia is native to Africa and is an inland tropical fish belonging to the order Perciformes, the family Anchovy, and the genus Tilapia. Because tilapia has the characteristics of fast growth, omnivorous, few diseases, strong disease resistance, and wide adaptability, tilapia has become the most widely introduced fish species in the world. After China introduced tilapia in the 1970s, the tilapia industry has developed rapidly. In 2008, the aquaculture output of tilapia in my country reached 1.2 million tons, accounting for 60% of the world, making it one of the main foreign exchange-earning fish species for export in my country. With the increase of tilapia breeding density in my country and the decline of the quality of the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34A01K61/00
CPCY02A40/81
Inventor 吴伟陈家长瞿建宏
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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