Method for establishing full-female pelteobagrus fulvidraco breeding population

A technology for yellow catfish and female fish, which is applied in fish farming, application, climate change adaptation, etc., to achieve the effects of increasing the acquisition rate, shortening the breeding time, and achieving obvious economic and social benefits

Inactive Publication Date: 2013-05-01
WATER ENG ECOLOGICAL INST CHINESE ACAD OF SCI
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Problems solved by technology

As for the establishment of breeding populations of whole female yellow catfish, especially the integration of sex reversa

Method used

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  • Method for establishing full-female pelteobagrus fulvidraco breeding population
  • Method for establishing full-female pelteobagrus fulvidraco breeding population

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Embodiment Construction

[0021] The present invention will be further described below in conjunction with the accompanying drawings.

[0022] attached figure 1 A technical schematic diagram of the method for establishing a whole female yellow catfish breeding population is shown, with figure 2 Shown is the step flow block diagram of the method for setting up whole female yellow catfish breeding population, in conjunction with accompanying drawing, specifically discusses the technological process of the present invention as follows:

[0023] Step 1. Artificial oxytocin and artificial insemination: according to the needs of experiments or industrial production, take a certain number of soft common orpiment catfish (15-20 tails / kg) with no injury on the body surface, swollen abdomen, and give each fish to the base of the pectoral fin Perform two injections, the total injection dose is HCG2500~3000IU+LRH‐A per kg female fish 2 30~40ug+DOM6~10mg. The oxytocic agents and doses used are as follows: the f...

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Abstract

The invention discloses a method for establishing a full-female pelteobagrus fulvidraco breeding population, which comprises the following steps of: 1) manually hastening parturition and manually inseminating; 2) reversing sex; 3) identifying inheritance sex of the sex reversal pelteobagrus fulvidraco; 4) expanding the population; and 5) establishing the full-female pelteobagrus fulvidraco breeding population, so as to finally establish the full-female pelteobagrus fulvidraco breeding population. According to the invention, an integrating technology of hormone sex reversal combined with pelteobagrus fulvidraco chromosome peculiar molecular markers is adopted, so that XX physiological milter can be confirmed without adopting a test mating method and the breeding time is shortened; compared with the test mating method, one generation can be shortened; the gynogenesis step is omitted, so that the damage to the germ cell genetic material caused by manual induction gynogenesis is reduced; the yield of the XX raun is increased; and the economic and social benefits are obvious.

Description

technical field [0001] The invention relates to an artificial sex control technology of fish, which belongs to a method for physiological genetic reproduction of fish, and belongs to the technical field of fish sex control by techniques such as artificial sex reversal, screening, group expansion and the like. Background technique [0002] There are several methods for producing parthenogenetic females: artificial selection, direct estrogen-induced sex reversal, gynogenesis, and sex-specific DNA probe technology. However, manual selection is labor-intensive and cannot accurately distinguish between male and female fish. Estrogen directly induces sex reversal is to feed estrogen to fish fry, and under the action of hormone, the male fish will undergo sex reversal and become female fish. This method cannot achieve the goal of all females, and there is a risk of residual sex hormones, which is not conducive to production, and has been banned in many countries. The biggest diff...

Claims

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Application Information

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IPC IPC(8): A01K61/00A01K67/027
CPCY02A40/81
Inventor 刘汉勤管波田华徐江侯昌春
Owner WATER ENG ECOLOGICAL INST CHINESE ACAD OF SCI
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