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Method for massively producing all-female loaches

A technology for female loach and loach, which is applied in the field of large-scale production of whole female loach, can solve the problems of high labor cost and time cost, complex sex determination mechanism of fish, cumbersome screening methods, etc., and achieve simplification of cultivation method, time cost and manpower The effect of cost optimization and shortening the cultivation period

Inactive Publication Date: 2017-01-04
FISHERIES RES INST ANHUI ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

However, this method has a long screening period, and the screening method is relatively cumbersome, and its labor cost and time cost are relatively high for large-scale breeding
[0005] The mechanism of sex determination in fish is well known to be complex

Method used

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Embodiment 1

[0026] Present embodiment provides a kind of method of large-scale production whole female loach, comprises the following steps:

[0027] (1) Parental cultivation

[0028] Large-scale production of loach fry, screening healthy female fish as backup parents, specifically:

[0029] ① Cultivate for 7-10 days, the fry are 1.5-2.0cm long, screen once, and select large-size fry, with a retention rate of 50%;

[0030] ② Transfer the selected fry to stocking, and the stocking density is 4000 fish / m 2 , continue to cultivate for 7-10 days, the fry are 2-3cm long, screen once, and select large-scale fry, with a retention rate of 50%;

[0031] ③ Adjust the stocking density to 2000 / m 2 , continue to cultivate for 7-10 days, the fry are up to 3-5cm in length, screened once, and select large-scale fry, the retention rate is 50%, and the reserve parent is obtained;

[0032] ④Selection of winter slices: After clearing the pond in winter, select large-scale, standard-sized, and healthy ind...

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Abstract

The invention discloses a method for massively producing all-female loaches. The method for massively producing the loaches comprises the steps that healthy and good female fishes are screened for backup parents, prenatal intensive cultivation and artificial spawning inducement are conducted, and all-female diploid fries are obtained by adopting a gynogenesis method achieved through heterologous sperm stimulation and cold shock treatment; artemia larvae soaked with a methyltestosterone male sex hormone solution with the concentration of 50 mg / kg are continuously taken as baits for 30 days, and XXo+ female fishes in the loach fries are subjected to sex reversal to be XXo+ physiological male fishes; the full-female diploid normal fish fries mate with the physiological male fishes, and then the 100 full-female loaches are massively produced. Compared with the prior art, the method has the advantages that the full-female loaches are massively produced by combining a gynogenesis technology with a sex reversal technology, and therefore the per mu yield and the culturing benefit are significantly increased; by means of the gynogenesis technology, a pure line can be rapidly established, very rich materials can be provided for genetic breeding, the breeding cycle of the full-female loaches can be shortened, and the gynogenesis technology is suitable for the massive production requirements and easier to apply and popularize.

Description

technical field [0001] The invention relates to the technical field of aquaculture, in particular to a method for large-scale production of whole female loach. Background technique [0002] Loach (Misgurnus arguillicaudatus) is a treasure of aquatic products, the market demand gap is large, and the price is rising year by year. Loach meat is tender, delicious, nutritious, rich in minerals and multivitamins, and has a variety of medicinal values. The content of vitamin B is 3 to 4 times higher than that of yellow croaker and shrimp. It is known as "ginseng in water" among the people. Say. The protein content of loach is higher than that of most conventional farmed fish, and its comprehensive amino acid content is regarded as a good product for nourishing and strengthening the body. It is one of the best raw materials for processing animal protein powder. [0003] Loach is an important freshwater fish. In nature, loach females are larger than males. Under the same breeding ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81A01K61/00
Inventor 王永杰陈红莲程云生孙棠丽苏鹏飞
Owner FISHERIES RES INST ANHUI ACAD OF AGRI SCI
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