Method for obtaining normal mature sperm of eel

Abstract

A method of obtaining normal mature sperms of eel relates to a method of obtaining normal mature sperms of eel requiring manual adjusting and controlling to obtain normal mature sperms of eel. The invention comprises: performing backgrounding domestication and food calling on the received catadromous eel parent, causing wild eel to adapting manual breeding environment, increasing corporeity of the eel parent through food calling, storing more matter and energy, preparing for entering the seawater, characterized in that, the eel parent is performed with salinity domestication after being bred for 15 days under manual breeding environment, salinity domestication is transferred from low concentration to sea water; water temperature of a culture pond for the eels is controlled between 18 to 25 DEG C, light is shielded in the day and moonlight is seen at night, flowing water stimulation is performed at night, oxygen concentration in the water keeps more than 6 mg / l, in weak alkalinity water, content of ammonia and nitrogen is not more than 0.02 mg / l and 0.05 mg / l, eel males discharge seminal fluid naturally, normal mature sperms are obtained. The invention is used for fully artificial propagation for the eels.

Description

technical field [0001] The invention relates to a method for obtaining normal mature spermatozoa of eel under the condition of artificial regulation (ripening and labor without exogenous hormone). Background technique [0002] Eel (Anguilla japonica) belongs to the order Eel and Eelidae. It is a kind of migratory fish. It lives in two completely different living environments, freshwater and seawater. The reproductive groups are descended from rivers to the sea for reproductive migration in autumn and winter every year. , the gonads develop and mature during the migration after entering the sea, and reach the spawning ground to reproduce. Every winter, flocks of young eels enter the estuary from the ocean, travel upstream, reach the middle and upper reaches and enter the water bodies attached to the rivers and lakes to inhabit, grow and fatten until they reach sexual maturity and begin to migrate to the sea to complete a life process. [0003] The artificial reproduction of ...

AI Technical Summary

Problems solved by technology

[0004] To achieve full artificial reproduction of eels, we must first start with obtaining normal sperm and eggs. Since the sexual maturity and reproduction of eels are completed in the ocean, the eels mysteriously disappeared after entering the sea. So far, we have not been able to obtain eels to lay eggs naturally. Naturally mature sperm and eggs

[0005] At present, people rely on exogenous hormones such as carp pituitary, HCG, LHRH, dioxydone, androstenedione and other hormones for injection or embedding, and artificially induce ripening to achieve gonad maturation and induced labor, so as to obtain eel sperm and Eggs, due to the ripening effect of exogenous hormones, have sperm quality problems, which cannot fully guarantee the quality of sperm, which directly affects the survival rate of seedlings after fertilization. one of the keys to survival