25 results about "Hyaluronates" patented technology

The invention belongs to the technical field of biological medicine, and particularly relates to hyaluronic acid nano-microspheres as well as a preparation method and application thereof. According to the preparation method of the hyaluronic acid nano-microspheres, the hyaluronic acid nano-microspheres are prepared through interaction of nucleic acid, basic amino acid and hyaluronate in a specific proportion, the preparation process is optimized, and the prepared hyaluronic acid nano-microspheres are high in hyaluronic acid adding amount, high in skin permeability and good in skin care effect. The stability is good, and the application requirements in transdermal drug delivery drugs, skin care products and other products are met. Meanwhile, a non-covalent binding mode is adopted for preparing the nano-microspheres, the preparation operation is simple, the gelling property of the hyaluronate is effectively improved, the sticky feeling under high content is improved, and the application scene of hyaluronic acid in the field of skin care products is widened.

The present invention relates to ophthalmic formulations in which a synergistic combination of three different types of disinfectants (a polybiguanide compound, a quaternary ammonium compound and an antifungal / anti-acanthamoeba compound), in particular a combination of polyhexamethylene biguanide, polyquaternium-1 and myristoylaminopropyl dimethylamine, high concentrations of hyaluronates are enabled to provide better comfort and support for corneal health without compromising antimicrobial efficacy. The novel ophthalmic formulations support the use of hyaluronates at concentrations greater than 7.5 times as high as currently commercially available multi-purpose disinfectants.

A complex consisting of lipoic acid or a salt thereof, a chelating agent such as a salt of ethylene diamine tetraacetic acid (EDTA) or analogous compounds and an ester of ascorbic acid with long-chain fatty acids, in particular ascorbyl palmitate inhibits the pigmentation of teeth induced by chlorhexidine and salts thereof. In addition to the depigmenting complex, the formulations of the invention, in the form of gels, mouthwashes, toothpastes or periodontal sprays, may contain other ingredients such as hyaluronic acid or hyaluronates, glycerophosphocholine, sweeteners, flavourings, buffering agents, viscosifying agents, mucoadhesive and filming agents, solvents, co-solvents, surfactants.

The present invention relates to ophthalmic formulations wherein a synergistic combination of three different classes of disinfectants (a multimeric biguanide compound, a quaternary ammonium compound and an antifungal / anti-acanthamoeba compound, in particular a combination of polyhexamethylene biguanide, Polyquaternium-1 and myristamidopropyl dimethylamine) enables the use of high concentrations of hyaluronate for providing better comfort and support of corneal health without compromising antimicrobial efficacy. The novel ophthalmic formulations support the use of more than 7.5 times higher concentrations of hyaluronate than are present in currently marketed multi-purpose disinfection solutions.

The invention discloses a skin implant, the skin implant is a freeze-drying agent, the freeze-drying agent is prepared from the following components in parts by weight: 2-15 parts of a PEG-PLA copolymer and 0.05-4.0 parts of hyaluronate, the PEG-PLA copolymer is prepared from a single-terminal hydroxyl polyethylene glycol compound and lactide through a polymerization reaction, and the hyaluronate is prepared from the following components in parts by weight: 1-10 parts of a cross-linking agent, 1-10 parts of a cross-linking agent, 1-10 parts of a cross-linking agent and 1-10 parts of a cross-linking agent. The mass ratio of the single-end hydroxyl polyethylene glycol to the lactide is (2.0-8.5): 1, and the molecular weight of the hyaluronate is 10-100 WDa. The skin implant provided by the invention has an excellent redissolution speed and can be quickly redissolved within 10 minutes, and the quick redissolution characteristic of the freeze-drying agent greatly facilitates clinical operation and shortens the preoperative preparation time.

The present invention relates to a hyaluronate non-woven fabric made of hyaluronate fibers and a method of manufacturing the same. More particularly, the present invention relates to a highly functional hyaluronate non-woven fabric made of hyaluronate fibers having a rough surface and a micro-size diameter and a method of manufacturing the same. A hyaluronate non-woven fabric manufacturing method according to the present invention includes (a) preparing hyaluronate fibers, (b) treating the hyaluronate fibers to be curled, (c) cutting the curled hyaluronate fibers, (d) forming a hyaluronate web from the curled hyaluronate fibers, and (e) needle-punching the hyaluronate web. The hyaluronate non-woven fabric of the present invention has a high hyaluronate content, is manufactured by needle punching high-strength hyaluronate fibers, and has excellent adhesion and tensile strength. Therefore, the hyaluronate non-woven fabric of the present invention can be used as a mask pack for external application on the skin, a biomaterial for tissue repairing, and an anti-adhesion coating material.

A biocompatible lyophilized pellet comprised of alginate and hyaluronate. The pellet may be used to provide a gliding surface for tissues impacted by surgery. The pellet may be delivered in a broad range of volumes to address large to micron-sized target tissues. The pellet may be hydrated before or after application. An external stimulus comprising a crosslinker may be applied to mold the pellet in situ around complex tissue geometries or to tailor the rate of bioresorption.

To provide a method for producing hyaluronate-containing fine fibers that are excellent in moisture retention and hygroscopicity and contain high-molecular-weight hyaluronate at a high concentration, hyaluronate-containing fine fibers, and a textile product.SOLUTION: A method for producing hyaluronate-containing fine fibers comprises: a step of preparing a mixed aqueous solution containing a hyaluronate having a weight average molecular weight of 500,000 or more, a polymer having a hydrophilic group and water; and an electro-spinning of injecting the mixed aqueous solution charged from a spinning nozzle on which a high voltage is applied to spin hyaluronate-containing fine fibers. The hyaluronate-containing fine fibers contain a hyaluronate having a weight average molecular weight of 500,000 or more and a polymer having a hydrophilic group, and the hyaluronate is contained in an amount of 10 mass% or more based on a total amount of the fibers.SELECTED DRAWING: Figure 1

The invention discloses a hydrogel sensing material for hyaluronidase activity detection and a preparation method and application thereof, and belongs to the technical field of biosensors. The hydrogel sensing material for hyaluronidase activity detection comprises: a three-dimensional network structure formed by crosslinking hyaluronate and polyethyleneimine; methylene blue molecules are loaded in the three-dimensional network structure. Based on the amount of the raw materials, the mass ratio of the hyaluronate to the methylene blue is 24-60; the mass ratio of the hyaluronate to the polyethyleneimine is 2 to 5. According to the method for quantitatively detecting hyaluronidase by using the hydrogel sensing material for hyaluronidase activity detection, the activity concentration of hyaluronidase can be rapidly and sensitively detected, the hyaluronidase activity detection can be realized only in 8-15 minutes, the specificity is good, and the anti-interference performance is strong.

This application belongs to the field of biomedical technology, specifically relating to hyaluronic acid nanospheres, their preparation methods, and applications. This application provides a method for preparing hyaluronic acid nanospheres, which involves the interaction of nucleic acids with hyaluronic acid salts in a specific ratio. The preparation process is optimized, resulting in hyaluronic acid nanospheres with high hyaluronic acid content, strong skin permeability, and good stability, meeting the application requirements for transdermal drug delivery and skincare products. Furthermore, this application uses a non-covalent bonding method to prepare the nanospheres, simplifying the preparation process, effectively improving the gelling properties of hyaluronic acid salts, reducing stickiness at high concentrations, and broadening the application scenarios of hyaluronic acid in skincare products.

The invention relates to a preparation method of hydrogel loaded with lidocaine hydrochloride and pregabalin and the hydrogel. The preparation method comprises the following steps: mixing hyaluronic acid, dopamine hydrochloride and pure water to prepare a solution A, and then preparing a nano cellulose suspension through an ultrasonic dispersion and magnetic stirring method; the preparation method comprises the following steps: preparing a nano-cellulose suspension, mixing and stirring lidocaine hydrochloride, pregabalin, carboxymethyl chitosan and the nano-cellulose suspension to prepare a solution B, mixing the solution A with the solution B, and carrying out step-by-step oxidation crosslinking treatment to finally obtain the hydrogel loaded with lidocaine hydrochloride and pregabalin. According to the invention, the mechanical strength and adhesion strength of the hydrogel are improved, the drug loading rate, drug loading stability and release controllability are ensured, and the synergistic treatment effect of first rapid analgesia and then long-acting nerve regulation is realized.

The application discloses a preparation method and application of a lysine hyaluronate composition, and belongs to the technical field of cosmetics. The preparation method comprises the following steps: preparing lysine hyaluronate, preparing a HA-LYS compound, preparing a tannic acid-magnesium compound, and mixing. The HA-LYS compound is prepared in the following manner: zinc acetate is mixed with an acetic acid aqueous solution, stirring is conducted at room temperature, chitosan is added, stirring is conducted, the pH value is adjusted to 5.8-6.2, HA-LYS is added, stirring is conducted, standing is conducted, alcohol precipitation is conducted, centrifugal separation is conducted, the precipitate is taken, the precipitate is washed with an ethanol aqueous solution, drying is conducted under reduced pressure, and crushing is conducted to obtain the HA-LYS compound. The lysine hyaluronate composition prepared in the application has the characteristics of good stability, good water solubility, good skin permeability, no harmful reagent residue, and a green and simple preparation process.

The invention discloses joint health-care soft sweets, and belongs to the field of health-care foods. Comprising hyaluronic acid or hyaluronate, a glucosamine compound without strongly acidic anions, a sugar body, a colloid, an additive and purified water. Compared with the prior art, firstly, glucosamine compounds, such as acetyl sugar or lemon glucosamine, which are milder in metabolism are selected as nutrient elements of the health-care soft sweets, so that the problem of stomach stimulation caused by introduction of sulfate radicals or chlorine radicals is fundamentally avoided, and damage to gastric mucosa can be reduced; secondly, the glucosamine and the hyaluronic acid are compounded according to a specific proportion, have a synergistic effect and can jointly protect articular cartilage and lubricate joints; and finally, through a specific material pretreatment method and process, the technical problems of high viscosity, moisture absorption, caking and the like caused by hyaluronic acid are solved, and the homogeneity, stability and industrial productivity of the composition in the soft sweets are ensured.

A biocompatible lyophilized pellet comprised of alginate and hyaluronate. The pellet may be used to provide a gliding surface for tissues impacted by surgery. The pellet may be delivered in a broad range of volumes to address large to micron-sized target tissues. The pellet may be hydrated before or after application. An external stimulus comprising a crosslinker may be applied to mold the pellet in situ around complex tissue geometries or to tailor the rate of bioresorption.

The present invention relates to a hyaluronate nanofabric sheet and a manufacturing method therefor and, more particularly, to a hyaluronate nanofabric sheet having excellent percutaneous absorption and physical properties and a manufacturing method therefor. A method for manufacturing a hyaluronate nanofabric sheet according to the present invention comprises the steps of: (a) mixing a hyaluronate having a weight average molecular weight of 5×103 - 5×104 Da and a hyaluronate having a weight average molecular weight of 1×105 - 1×106Da and dissolving same in water to prepare an aqueous hyaluronate solution; and (b) electrospinning the aqueous hyaluronate solution. The hyaluronate nanofabric sheet of the present invention has an excellent transdermal absorption rate due to the ultra-low molecular weight hyaluronic acid as a main component thereof and exhibits excellent physical properties due to a certain amount of medium-molecular weight hyaluronic acid mixed therewith, and as such, can be applied for various purposes, such as mask patches for topical uses, ophthalmic or mucosal drug delivery systems, etc.

Disclosed in the present invention is a method for preparing a controllable fibroin extracellular scaffold gel material for injection. A composite filler consists of the following raw materials in mass ratio: 5-50 parts of fibroin, 0.01-5 parts of cellulose, and 0.7-10 parts of a hyaluronate. The method comprises: (1) preparation of a fibroin microfiber gel: adding cellulose to an aqueous fibroin solution to prepare a gel, and crushing and sieving to give the fibroin microfiber gel; (2) preparation of an extracellular scaffold gel material: mixing a hyaluronate with the fibroin microfiber gel to physically crosslink same, so as to form the fibroin extracellular scaffold gel material. The modified fibroin hydrogel prepared by the method can promote in-situ tissue regeneration after injection and features a controllable molecular weight and a controllable crosslinking degree. The modified fibroin hydrogel possesses a structure similar to that of the extracellular matrix (ECM), a degradation process matching the in-situ tissue regeneration process, and improved mechanical properties, degradation properties, biocompatibility, etc., without the use of chemical crosslinking agents. The modified fibroin hydrogel is injectable and prospective in the field of medical beauty injection.

The application belongs to the field of new daily chemical materials, and discloses a skin introduction preparation containing trehalose, full-molecular-weight hyaluronate and hydroxytyrosol; the mass ratio of trehalose to full-molecular-weight hyaluronate is 1:99-99:1; and the mass ratio of hydroxytyrosol to full-molecular-weight hyaluronate is 20:1-200. The preparation introduces full-molecular-weight hyaluronate as one of core components, and improves the transdermal effect of trehalose and hydroxytyrosol through the full-molecular-weight hyaluronate. The active ingredient hydroxytyrosol in the preparation can effectively remove free radicals and decompose melanin to lighten and eliminate color spots. The trehalose and full-molecular-weight hyaluronate in the preparation can repair damaged skin, relieve inflammation and resist aging. The three components jointly act to achieve a synergistic skin repair effect. Meanwhile, the application also provides a preparation method and application of the transdermal introduction preparation.

The application discloses a preparation method of controllable silk fibroin extracellular matrix gel material for injection. The composite filler is composed of raw materials in the following mass ratio: 5-50 parts of silk fibroin, 0.01-5 parts of cellulose, and 0.7-10 parts of hyaluronate. The preparation method comprises the following steps: (1) preparation of silk fibroin microfiber gel: adding silk fibroin aqueous solution into cellulose to prepare a gel, crushing, and sieving to obtain silk fibroin microfiber gel; (2) preparation of extracellular matrix gel material: mixing hyaluronate with the silk fibroin microfiber gel, physically cross-linking the mixture, and forming silk fibroin extracellular matrix gel material. The modified silk fibroin hydrogel prepared by the method can promote in-situ tissue regeneration after injection, the molecular weight and cross-linking degree of the modified silk fibroin hydrogel are controllable, the structure of the modified silk fibroin hydrogel is similar to the structure of extracellular matrix (ECM), the degradation process of the modified silk fibroin hydrogel is matched with the in-situ tissue regeneration process, the mechanical property, degradation property and biocompatibility of the modified silk fibroin hydrogel are improved, no chemical cross-linking agent is used, and the modified silk fibroin hydrogel is injectable, so the modified silk fibroin hydrogel has a good application prospect in the field of medical and beauty injection.

The invention discloses application of hyaluronic acid and salt thereof in enhancing salty, fresh, sweet, bitter and sour tastes, and belongs to the field of salt reduction and sugar reduction. The invention discloses application of hyaluronic acid, hyaluronate and a mixture of the hyaluronic acid and the hyaluronate in taste enhancement, the hyaluronate comprises sodium salt and the like, and the molecular weight of the hyaluronate is 80.4 kDa to 1090 kDa. Based on this use, it is possible for the animal body, especially the human body, to reduce the intake of the condiment or flavoring compounds in the condiment while obtaining the same taste sensation, or to obtain a more lasting taste sensation while taking the same amount of condiment or flavoring compound.

The invention belongs to the technical field of sodium hyaluronate compounds, and particularly relates to a compound composition of sodium hyaluronates with different molecular weights, a preparation method and application. The compound composition comprises a gradient hyaluronic acid group, an interface compound group, a continuous phase adjusting group and an auxiliary stabilizing group, and the gradient hyaluronic acid group comprises high molecular weight sodium hyaluronate, medium molecular weight sodium hyaluronate, low molecular weight sodium hyaluronate and oligomeric sodium hyaluronate. The interface compounding group comprises a phospholipid component and an amphoteric small molecule component, and the continuous phase adjusting group comprises a saccharide component, a polyol component, a buffer salt component and water. According to the present invention, the compatibility relationship and the compounding path among the components are clearer through the grouping construction and the step-by-step compounding mode, and the obtained composition has characteristics of good system uniformity, good dispersion coordination and good stability, and can be used for gel preparations, spray preparations, emulsion preparations, cream preparations, application preparations, medical dressing compositions and external liquid preparations.

To provide a hyaluronate skin-penetrating cosmetic that allows a hyaluronate to penetrate into the skin without a complicated step such as composite formation of a hyaluronate.A hyaluronate skin-penetrating cosmetic of the present disclosure contains a hyaluronate and an amphoteric surfactant.

The application belongs to the technical field of medical biomaterials, and particularly relates to a hyaluronic acid particle hydrogel as well as a preparation method and application thereof. The hyaluronic acid particle hydrogel is prepared by using hyaluronate and 1,4-butanediol diglycidyl ether as raw materials, and by reacting a mixed aqueous solution of the two in an oil phase containing a fluorine surfactant. The prepared hyaluronic acid particle hydrogel has excellent mechanical properties and elasticity, and the anti-enzymatic degradation capability is greatly improved. The hyaluronic acid particle hydrogel can also significantly reduce the generation of aging cells, reduce the expression levels of inflammatory factors IL-6 and IL-1beta in chondrocytes, and play an excellent anti-inflammatory role. Moreover, the preparation method is simple, the conditions are mild, and the method is suitable for large-scale production.

The invention relates to the technical field of medical biological materials, and particularly discloses a gel microsphere with a core-shell structure and a preparation method thereof, the shell of the microsphere is hydrogel composed of at least one of hyaluronic acid or hyaluronate or a water-soluble high-molecular polymer, the inner core is an aqueous solution containing nutrient substances or drugs, and the core is an aqueous solution containing the nutrient substances or drugs. A two-stage microfluidic device is used, a low-viscosity aqueous solution containing nutrient substances or drugs serves as an inner phase, a high-viscosity hyaluronic acid solution serves as an intermediate phase, a coaxial layered solution is formed, then a low-viscosity calcium chloride aqueous solution serves as an outer phase, the coaxial layered solution is sheared, and the gel microspheres with the core-shell structure are obtained. According to the preparation method, the gel microspheres with uniform size and high core-shell concentricity can be prepared by utilizing the viscosity difference between the inner phase and the intermediate phase, using a microfluidic technology and adopting a full-water-phase system; the prepared gel microspheres do not need to be cleaned and dialyzed by an oil-phase organic solvent, so that the preparation efficiency is improved, and no oil-phase organic solvent is left.

Skin cream or skin cream serum, in particular face cream, for anti-aging treatment of the skin, comprising a formulation based on an emulsion of water and vegetable oil, characterized in that the formulation also comprises at least three active ingredients, comprising: a. one or more placenta-based peptides b. a tellumer activator and c. a mixture of two or more hyaluronic acid salts with different chain lengths

A biocompatible membrane comprised of alginate and hyaluronate. The membrane may be used to prevent unwanted scarring after surgery. The tissue adherence and the rate of bioresorption of the membrane may be modified through an external stimulus comprising a sequestering agent and a viscosity modifier.