71 results about "Hyaluronates" patented technology

The invention discloses a preparation method of a controllable silk fibroin extracellular skeleton gel material for injection. The composite filler is prepared from the following raw materials in parts by mass: 5-50 parts of silk fibroin, 0.01-5 parts of cellulose and 0.7-10 parts of hyaluronate. The preparation method comprises the following steps: (1) preparation of silk fibroin microfiber gel: adding cellulose into a silk fibroin aqueous solution to prepare gel, crushing and sieving to obtain the silk fibroin microfiber gel; and (2) preparation of an extracellular skeleton gel material: mixing hyaluronate with the silk fibroin microfiber gel, and carrying out physical crosslinking to form the silk fibroin extracellular skeleton gel material. After the modified silk fibroin hydrogel prepared by the method is injected, in-situ tissue regeneration can be promoted, the molecular weight is controllable, the crosslinking degree is controllable, the structure of the modified silk fibroin hydrogel is similar to that of an extracellular matrix (ECM), the degradation process is matched with the in-situ tissue regeneration process, and the modified silk fibroin hydrogel is well improved in the aspects of mechanical property, degradation performance, biocompatibility and the like and has good application prospects. No chemical cross-linking agent is used, and the injection has injectability, and has a very good application prospect in the field of medical beauty injection.

The invention relates to the technical field of biological products, in particular to a collagen-hyaluronic acid or hyaluronate composite solution. By controlling the concentration of the collagen concentrated solution, the concentration of the hyaluronic acid / hyaluronate solution and the mass ratio of the collagen to the hyaluronic acid / hyaluronate in the composite solution, the composite solution has good storage stability and whiteness and is easy to push. The composite solution is safe to use, simple in preparation method and easy to industrialize, can be used in the field of injection medical treatment or injection filling, and has a wide application prospect.

The invention provides a method for preparing ultra-small molecular zinc hyaluronate, and relates to the field of hyaluronate. The method comprises the following steps: carrying out enzymolysis on macromolecular sodium hyaluronate by using leech hyaluronidase to obtain ultra-small molecular sodium hyaluronate; the method comprises the following steps: adsorbing ultra-small molecular sodium hyaluronate by using anion exchange resin, eluting by using a zinc salt solution, and reacting the obtained eluent to obtain ultra-small molecular zinc hyaluronate; the average molecular weight of the ultra-small molecular sodium hyaluronate is 600 Da to 1000 Da. The whole process is simple, the obtained ultra-small molecular zinc hyaluronate is high in zinc content, free of sodium ion residues and high in preparation yield, an organic solvent is not needed in the whole production process, and the method is suitable for industrial production.

The invention discloses a preparation method of low-temperature cross-linked sodium hyaluronate gel particles, which comprises the following steps: 1, mixing two sodium hyaluronates with different molecular weights, adding the mixture into a cross-linking reaction medium, and uniformly stirring and dispersing to obtain a sodium hyaluronate cross-linking reaction system mixed solution; 2, performing low-temperature standing and cross-linking reaction to obtain cross-linked sodium hyaluronate gel; 3, roughly cutting gel blocks, performing constant-volume purification, cleaning and draining to obtain cross-linked sodium hyaluronate gel; and 4, extruding and granulating to obtain the cross-linked sodium hyaluronate gel particles. In addition, the invention also discloses an application of the low-temperature cross-linked sodium hyaluronate gel particle. The mixed cross-linked gel is prepared under the conditions of sodium hyaluronates with different molecular weights and low-temperature standing, the cross-linking degree of the prepared gel can reach 20% or above, the elastic modulus can reach 400 Pa or above, the residual quantity of a cross-linking agent is smaller than 0.1 ppm, and the gel can be used for preparing a sodium hyaluronate gel product for injection and filling.

The invention discloses a film-forming agent composition as well as a preparation method and application of the film-forming agent composition. The film-forming agent composition is prepared from the following components in percentage by weight: 0.2 to 2 percent of hyaluronic acid and / or hyaluronate, 15 to 40 percent of polyhydric alcohol, 0.5 to 2 percent of gluconolactone and / or lactobionic acid lactone, and the balance of deionized water. The film-forming agent composition provided by the invention is obtained by adding a proper amount of polyol, gluconolactone and / or lactobionic acid lactone into an aqueous solution of active substance hyaluronic acid and / or hyaluronate, the polyhydric alcohol, gluconolactone and / or lactobionic acid lactone can form hydrogen bonds with the active substances hyaluronic acid and hyaluronate, and a complex hydrogen bond net can be formed by mixing the polyhydric alcohol, gluconolactone and / or lactobionic acid lactone with the active substances hyaluronic acid and hyaluronate, so that the flexibility of the film-forming agent composition can be greatly improved, the film-forming effect is good, the comprehensive performance of a formed film is excellent, and the film-forming agent composition can be widely applied to the field of film forming. The compound can be used as a film-forming agent to be applied to cosmetics and skin care products in the field of cosmetics and medicines in the field of medicines.

The invention belongs to the technical field of biological medicine, and particularly relates to hyaluronic acid nano-microspheres as well as a preparation method and application thereof. According to the preparation method of the hyaluronic acid nano-microspheres, the hyaluronic acid nano-microspheres are prepared through interaction of nucleic acid, basic amino acid and hyaluronate in a specific proportion, the preparation process is optimized, and the prepared hyaluronic acid nano-microspheres are high in hyaluronic acid adding amount, high in skin permeability and good in skin care effect. The stability is good, and the application requirements in transdermal drug delivery drugs, skin care products and other products are met. Meanwhile, a non-covalent binding mode is adopted for preparing the nano-microspheres, the preparation operation is simple, the gelling property of the hyaluronate is effectively improved, the sticky feeling under high content is improved, and the application scene of hyaluronic acid in the field of skin care products is widened.

The present invention relates to ophthalmic formulations in which a synergistic combination of three different types of disinfectants (a polybiguanide compound, a quaternary ammonium compound and an antifungal / anti-acanthamoeba compound), in particular a combination of polyhexamethylene biguanide, polyquaternium-1 and myristoylaminopropyl dimethylamine, high concentrations of hyaluronates are enabled to provide better comfort and support for corneal health without compromising antimicrobial efficacy. The novel ophthalmic formulations support the use of hyaluronates at concentrations greater than 7.5 times as high as currently commercially available multi-purpose disinfectants.

A complex consisting of lipoic acid or a salt thereof, a chelating agent such as a salt of ethylene diamine tetraacetic acid (EDTA) or analogous compounds and an ester of ascorbic acid with long-chain fatty acids, in particular ascorbyl palmitate inhibits the pigmentation of teeth induced by chlorhexidine and salts thereof. In addition to the depigmenting complex, the formulations of the invention, in the form of gels, mouthwashes, toothpastes or periodontal sprays, may contain other ingredients such as hyaluronic acid or hyaluronates, glycerophosphocholine, sweeteners, flavourings, buffering agents, viscosifying agents, mucoadhesive and filming agents, solvents, co-solvents, surfactants.

A cosmetic composition comprises at least one Laminaria extract and at least one salt of hydrolyzed hyaluronate, for topical application to skin for seborrhea control.

The present application provides a method for preparing a fast-dissolving hyaluronate, wherein the fast-dissolving hyaluronate is prepared using hyaluronate and a binder as raw materials, wherein the binder is selected from one or more of monosaccharides, disaccharides, oligosaccharides, sugar alcohols, and soluble dietary fibers. The preparation method of the present application can solve the problems of dust pollution and slow dissolution rate during the use of hyaluronate. The preparation method of the present application granulates the hyaluronate to prevent the generation of small particles of dust, and can be quickly dispersed and dissolved in a liquid, thereby saving time and cost. In addition, the preparation method of the present application does not use an organic solvent and is safer.

Pharmaceutical compositions in the form of dry powder for inhalation comprising a local anesthetic such as procaine, chloroprocaine, lidocaine, prilocaine, mepivacaine, bupivacaine, etidocaine, ropivacaine, and tetracaine or salts and / or solvates thereof and a hydrophilic biocompatible polymer, in particular hyaluronates, are described. The compositions of the invention are useful for the treatment of the cough.

The present invention relates to ophthalmic formulations wherein a synergistic combination of three different classes of disinfectants (a multimeric biguanide compound, a quaternary ammonium compound and an antifungal / anti-acanthamoeba compound, in particular a combination of polyhexamethylene biguanide, Polyquaternium-1 and myristamidopropyl dimethylamine) enables the use of high concentrations of hyaluronate for providing better comfort and support of corneal health without compromising antimicrobial efficacy. The novel ophthalmic formulations support the use of more than 7.5 times higher concentrations of hyaluronate than are present in currently marketed multi-purpose disinfection solutions.

The invention discloses a skin implant, the skin implant is a freeze-drying agent, the freeze-drying agent is prepared from the following components in parts by weight: 2-15 parts of a PEG-PLA copolymer and 0.05-4.0 parts of hyaluronate, the PEG-PLA copolymer is prepared from a single-terminal hydroxyl polyethylene glycol compound and lactide through a polymerization reaction, and the hyaluronate is prepared from the following components in parts by weight: 1-10 parts of a cross-linking agent, 1-10 parts of a cross-linking agent, 1-10 parts of a cross-linking agent and 1-10 parts of a cross-linking agent. The mass ratio of the single-end hydroxyl polyethylene glycol to the lactide is (2.0-8.5): 1, and the molecular weight of the hyaluronate is 10-100 WDa. The skin implant provided by the invention has an excellent redissolution speed and can be quickly redissolved within 10 minutes, and the quick redissolution characteristic of the freeze-drying agent greatly facilitates clinical operation and shortens the preoperative preparation time.

The present invention relates to a hyaluronate non-woven fabric made of hyaluronate fibers and a method of manufacturing the same. More particularly, the present invention relates to a highly functional hyaluronate non-woven fabric made of hyaluronate fibers having a rough surface and a micro-size diameter and a method of manufacturing the same. A hyaluronate non-woven fabric manufacturing method according to the present invention includes (a) preparing hyaluronate fibers, (b) treating the hyaluronate fibers to be curled, (c) cutting the curled hyaluronate fibers, (d) forming a hyaluronate web from the curled hyaluronate fibers, and (e) needle-punching the hyaluronate web. The hyaluronate non-woven fabric of the present invention has a high hyaluronate content, is manufactured by needle punching high-strength hyaluronate fibers, and has excellent adhesion and tensile strength. Therefore, the hyaluronate non-woven fabric of the present invention can be used as a mask pack for external application on the skin, a biomaterial for tissue repairing, and an anti-adhesion coating material.

A biocompatible lyophilized pellet comprised of alginate and hyaluronate. The pellet may be used to provide a gliding surface for tissues impacted by surgery. The pellet may be delivered in a broad range of volumes to address large to micron-sized target tissues. The pellet may be hydrated before or after application. An external stimulus comprising a crosslinker may be applied to mold the pellet in situ around complex tissue geometries or to tailor the rate of bioresorption.

The invention discloses a method for preparing silk fibroin and hyaluronic acid composite gel, which comprises the following steps of: uniformly mixing hyaluronate with a first solvent to prepare hyaluronic acid gel, mixing the hyaluronic acid gel with a silk fibroin solution, shearing at a high speed, homogenizing and emulsifying, standing, shearing at a high speed, homogenizing and emulsifying, sealing and sterilizing to obtain the silk fibroin and hyaluronic acid composite gel. According to the method disclosed by the invention, reagents such as a cross-linking agent, an initiator and enzyme do not need to be additionally added; the silk fibroin solution and the hyaluronic acid gel can form uniform and mutually-crosslinked double networks in the homogeneous gel forming process, the phase separation risk is low, and the silk fibroin can generate an emulsion reaction through high-speed homogenization, so that the color of the composite gel is whiter; the preparation method disclosed by the invention is simple, rapid in emulsification and gel forming, short in production cycle, low in endotoxin of the product, high in biocompatibility, slow in degradation, small in inflammatory response and high in injection filling safety, and the composite gel can play a role in moisturizing and moisturizing after subcutaneous injection and has the whitening effects of improving skin elasticity and glossiness, fading fine wrinkles and the like.

The invention provides a preparation method of semi-fluid micro-crosslinked gel, which comprises the following steps: dissolving a mixture of sodium hyaluronates with various different molecular weights in an alkaline solution, and centrifuging after the mixture is completely dissolved, so that the sodium hyaluronates with various different molecular weights are longitudinally arranged to realize longitudinal gradient distribution; the preparation method comprises the following steps: adding a cross-linking agent dropwise in a centrifugal state to form a layered gradient cross-linked structure, further introducing ultrasonic treatment to induce transverse rearrangement of molecules, fully utilizing the limited cross-linking agent to carry out cross-linking again, improving the utilization rate of the cross-linking agent, enhancing the mechanical property of the gel and reducing the residue of the cross-linking agent, and finally carrying out post-treatment to obtain the semi-fluid micro-cross-linked gel. The semi-fluid micro-crosslinking gel prepared by the preparation method disclosed by the invention has good mechanical properties and biological integration.

A method of making a semi-synthetic scaffold is described. The method includes mixing a gelatin solution, a high molecular weight chitosan solution, and a hyaluronate solution, to form a polymeric liquid solution; pouring the polymeric liquid solution into a mold; gradually cooling the mixture to gellify and form a polymer matrix; and freezing and lyophilizing the polymer matrix to remove water and form a scaffold. Semi-synthetic scaffolds, and methods of using them for wound healing, are also described.

To prevent unwanted scarring after surgery.SOLUTION: A biocompatible membrane comprising alginate and hyaluronate is provided. The membrane may be used to prevent unwanted scarring after surgery. The tissue adherence and the rate of bioresorption of the membrane may be modified through an external stimulus comprising a sequestering agent and a viscosity modifier.SELECTED DRAWING: None

The invention provides a liquid dressing and a preparation method thereof, and belongs to the technical field of skin barrier repair and treatment.The liquid dressing comprises four phases of raw materials, a phase D comprises p-hydroxyacetophenone, 1, 2-hexanediol, 1, 3-butanediol and the like, and a phase D comprises p-hydroxyacetophenone, 1, 2-hexanediol, 1, 3-butanediol and the like; the phase C comprises triethanolamine, deionized water and the like; the phase B comprises allantoin, carbomer, various sodium hyaluronates, glycerol and the like; the phase A comprises cetostearyl alcohol, beheneth-22 and the like. The preparation method comprises the following steps: heating the phase A to 80-85 DEG C; heating the phase B to 80-85 DEG C, and keeping the temperature for 20 minutes; adding the phase B into a homogenizer, stirring, adding the phase A, emulsifying at 80 DEG C, cooling and homogenizing; cooling to 75-60 DEG C, adding the phase C, and homogenizing; cooling to below 45 DEG C, adding the phase D, and uniformly stirring; the dressing can form a skin defense film, protect a corium layer, deeply preserve moisture and block bacteria, sodium hyaluronate has the effects of resisting inflammation, repairing and the like, and butyrospermum parkii butter can moisten skin and improve skin resistance.

The application discloses a use of hyaluronic acid and its salt in taste enhancement, and belongs to the field of salt and sugar reduction. The application discloses a use of hyaluronic acid, hyaluronic acid salt and a mixture of hyaluronic acid and hyaluronic acid salt in taste enhancement, wherein the hyaluronic acid salt includes sodium salt and the like, and the molecular weight is 80.4 kDa-1090 kDa. Based on the use, the intake of condiments or taste compounds in condiments of an animal body, especially a human body, can be reduced under the condition of obtaining the same taste feeling, or a more lasting feeling of taste can be obtained under the condition of intaking the same amount of condiments or taste compounds.

To provide a hyaluronate skin-penetrating cosmetic that allows a hyaluronate to penetrate into the skin without a complicated step such as composite formation of a hyaluronate.A hyaluronate skin-penetrating cosmetic of the present disclosure contains a hyaluronate and an amphoteric surfactant.

The present invention relates to a cosmetic composition for topical application to the skin to control sebum overflow, comprising at least one kelp extract and at least one hydrolyzed hyaluronate.

The invention discloses an oral care composition which comprises hyaluronic acid and / or a derivative thereof and a ginseng root extract, and the weight ratio of the hyaluronic acid and / or the derivative thereof to the ginseng root extract is 50: 1 to 10000: 1; and wherein the hyaluronic acid derivative is a hyaluronate salt in the form of a salt having a cation selected from the group consisting of a sodium ion, a potassium ion, a zinc ion, an ammonium ion, mono-, di-, tri-alkanolamine, and combinations thereof.

The present invention relates to a hyaluronate non-woven fabric made of hyaluronate fibers and a method of manufacturing the same. More particularly, the present invention relates to a highly functional hyaluronate non-woven fabric made of hyaluronate fibers having a rough surface and a micro-size diameter and a method of manufacturing the same. A hyaluronate non-woven fabric manufacturing method according to the present invention includes (a) preparing hyaluronate fibers, (b) treating the hyaluronate fibers to be curled, (c) cutting the curled hyaluronate fibers, (d) forming a hyaluronate web from the curled hyaluronate fibers, and (e) needle-punching the hyaluronate web. The hyaluronate non-woven fabric of the present invention has a high hyaluronate content, is manufactured by needle punching high-strength hyaluronate fibers, and has excellent adhesion and tensile strength. Therefore, the hyaluronate non-woven fabric of the present invention can be used as a mask pack for external application on the skin, a biomaterial for tissue repairing, and an anti-adhesion coating material.

The invention relates to a composition for promoting gene expression of skin cells in the aspects of moisturizing and repairing. The composition is composed of ceramide, composite hyaluronic acid and cosmetic acceptable auxiliary materials, ceramide is selected from ceramide 1 to ceramide 11, and the composite hyaluronic acid is a compound of hyaluronic acid and two sodium hyaluronates with different molecular weights. According to the composition of the composite hyaluronic acid and the ceramide, disclosed by the invention, the effects of moisturizing and repairing the skin can be synergistically exerted. And compared with single use of the two components, the effect is better.

To provide a method for producing hyaluronate-containing fine fibers that are excellent in moisture retention and hygroscopicity and contain high-molecular-weight hyaluronate at a high concentration, hyaluronate-containing fine fibers, and a textile product.SOLUTION: A method for producing hyaluronate-containing fine fibers comprises: a step of preparing a mixed aqueous solution containing a hyaluronate having a weight average molecular weight of 500,000 or more, a polymer having a hydrophilic group and water; and an electro-spinning of injecting the mixed aqueous solution charged from a spinning nozzle on which a high voltage is applied to spin hyaluronate-containing fine fibers. The hyaluronate-containing fine fibers contain a hyaluronate having a weight average molecular weight of 500,000 or more and a polymer having a hydrophilic group, and the hyaluronate is contained in an amount of 10 mass% or more based on a total amount of the fibers.SELECTED DRAWING: Figure 1

A biocompatible membrane comprised of alginate and hyaluronate. The membrane may be used to prevent unwanted scarring after surgery. The tissue adherence and the rate of bioresorption of the membrane may be modified through an external stimulus comprising a sequestering agent and a viscosity modifier.

A pharmaceutical composition in the form of a dry powder for inhalation is described, which comprises a local anesthetic, such as procaine, chloroprocaine, lidocaine, prilocaine, mepivacaine, bupivacaine, etidocaine, ropivacaine, and tetracaine, or their salts and / or solvates, and a hydrophilic biocompatible polymer, in particular hyaluronate.The composition of the present invention is useful for the treatment of cough.

The invention discloses a hydrogel sensing material for hyaluronidase activity detection and a preparation method and application thereof, and belongs to the technical field of biosensors. The hydrogel sensing material for hyaluronidase activity detection comprises: a three-dimensional network structure formed by crosslinking hyaluronate and polyethyleneimine; methylene blue molecules are loaded in the three-dimensional network structure. Based on the amount of the raw materials, the mass ratio of the hyaluronate to the methylene blue is 24-60; the mass ratio of the hyaluronate to the polyethyleneimine is 2 to 5. According to the method for quantitatively detecting hyaluronidase by using the hydrogel sensing material for hyaluronidase activity detection, the activity concentration of hyaluronidase can be rapidly and sensitively detected, the hyaluronidase activity detection can be realized only in 8-15 minutes, the specificity is good, and the anti-interference performance is strong.