50 results about "Hyaluronates" patented technology

The invention relates to the technical field of biological products, in particular to a collagen-hyaluronic acid or hyaluronate composite solution. By controlling the concentration of the collagen concentrated solution, the concentration of the hyaluronic acid / hyaluronate solution and the mass ratio of the collagen to the hyaluronic acid / hyaluronate in the composite solution, the composite solution has good storage stability and whiteness and is easy to push. The composite solution is safe to use, simple in preparation method and easy to industrialize, can be used in the field of injection medical treatment or injection filling, and has a wide application prospect.

The invention provides a method for preparing ultra-small molecular zinc hyaluronate, and relates to the field of hyaluronate. The method comprises the following steps: carrying out enzymolysis on macromolecular sodium hyaluronate by using leech hyaluronidase to obtain ultra-small molecular sodium hyaluronate; the method comprises the following steps: adsorbing ultra-small molecular sodium hyaluronate by using anion exchange resin, eluting by using a zinc salt solution, and reacting the obtained eluent to obtain ultra-small molecular zinc hyaluronate; the average molecular weight of the ultra-small molecular sodium hyaluronate is 600 Da to 1000 Da. The whole process is simple, the obtained ultra-small molecular zinc hyaluronate is high in zinc content, free of sodium ion residues and high in preparation yield, an organic solvent is not needed in the whole production process, and the method is suitable for industrial production.

The invention discloses a preparation method of low-temperature cross-linked sodium hyaluronate gel particles, which comprises the following steps: 1, mixing two sodium hyaluronates with different molecular weights, adding the mixture into a cross-linking reaction medium, and uniformly stirring and dispersing to obtain a sodium hyaluronate cross-linking reaction system mixed solution; 2, performing low-temperature standing and cross-linking reaction to obtain cross-linked sodium hyaluronate gel; 3, roughly cutting gel blocks, performing constant-volume purification, cleaning and draining to obtain cross-linked sodium hyaluronate gel; and 4, extruding and granulating to obtain the cross-linked sodium hyaluronate gel particles. In addition, the invention also discloses an application of the low-temperature cross-linked sodium hyaluronate gel particle. The mixed cross-linked gel is prepared under the conditions of sodium hyaluronates with different molecular weights and low-temperature standing, the cross-linking degree of the prepared gel can reach 20% or above, the elastic modulus can reach 400 Pa or above, the residual quantity of a cross-linking agent is smaller than 0.1 ppm, and the gel can be used for preparing a sodium hyaluronate gel product for injection and filling.

The invention belongs to the technical field of biological medicine, and particularly relates to hyaluronic acid nano-microspheres as well as a preparation method and application thereof. According to the preparation method of the hyaluronic acid nano-microspheres, the hyaluronic acid nano-microspheres are prepared through interaction of nucleic acid, basic amino acid and hyaluronate in a specific proportion, the preparation process is optimized, and the prepared hyaluronic acid nano-microspheres are high in hyaluronic acid adding amount, high in skin permeability and good in skin care effect. The stability is good, and the application requirements in transdermal drug delivery drugs, skin care products and other products are met. Meanwhile, a non-covalent binding mode is adopted for preparing the nano-microspheres, the preparation operation is simple, the gelling property of the hyaluronate is effectively improved, the sticky feeling under high content is improved, and the application scene of hyaluronic acid in the field of skin care products is widened.

The present invention relates to ophthalmic formulations in which a synergistic combination of three different types of disinfectants (a polybiguanide compound, a quaternary ammonium compound and an antifungal / anti-acanthamoeba compound), in particular a combination of polyhexamethylene biguanide, polyquaternium-1 and myristoylaminopropyl dimethylamine, high concentrations of hyaluronates are enabled to provide better comfort and support for corneal health without compromising antimicrobial efficacy. The novel ophthalmic formulations support the use of hyaluronates at concentrations greater than 7.5 times as high as currently commercially available multi-purpose disinfectants.

A complex consisting of lipoic acid or a salt thereof, a chelating agent such as a salt of ethylene diamine tetraacetic acid (EDTA) or analogous compounds and an ester of ascorbic acid with long-chain fatty acids, in particular ascorbyl palmitate inhibits the pigmentation of teeth induced by chlorhexidine and salts thereof. In addition to the depigmenting complex, the formulations of the invention, in the form of gels, mouthwashes, toothpastes or periodontal sprays, may contain other ingredients such as hyaluronic acid or hyaluronates, glycerophosphocholine, sweeteners, flavourings, buffering agents, viscosifying agents, mucoadhesive and filming agents, solvents, co-solvents, surfactants.

A cosmetic composition comprises at least one Laminaria extract and at least one salt of hydrolyzed hyaluronate, for topical application to skin for seborrhea control.

The present invention relates to ophthalmic formulations wherein a synergistic combination of three different classes of disinfectants (a multimeric biguanide compound, a quaternary ammonium compound and an antifungal / anti-acanthamoeba compound, in particular a combination of polyhexamethylene biguanide, Polyquaternium-1 and myristamidopropyl dimethylamine) enables the use of high concentrations of hyaluronate for providing better comfort and support of corneal health without compromising antimicrobial efficacy. The novel ophthalmic formulations support the use of more than 7.5 times higher concentrations of hyaluronate than are present in currently marketed multi-purpose disinfection solutions.

The invention discloses a skin implant, the skin implant is a freeze-drying agent, the freeze-drying agent is prepared from the following components in parts by weight: 2-15 parts of a PEG-PLA copolymer and 0.05-4.0 parts of hyaluronate, the PEG-PLA copolymer is prepared from a single-terminal hydroxyl polyethylene glycol compound and lactide through a polymerization reaction, and the hyaluronate is prepared from the following components in parts by weight: 1-10 parts of a cross-linking agent, 1-10 parts of a cross-linking agent, 1-10 parts of a cross-linking agent and 1-10 parts of a cross-linking agent. The mass ratio of the single-end hydroxyl polyethylene glycol to the lactide is (2.0-8.5): 1, and the molecular weight of the hyaluronate is 10-100 WDa. The skin implant provided by the invention has an excellent redissolution speed and can be quickly redissolved within 10 minutes, and the quick redissolution characteristic of the freeze-drying agent greatly facilitates clinical operation and shortens the preoperative preparation time.

The present invention relates to a hyaluronate non-woven fabric made of hyaluronate fibers and a method of manufacturing the same. More particularly, the present invention relates to a highly functional hyaluronate non-woven fabric made of hyaluronate fibers having a rough surface and a micro-size diameter and a method of manufacturing the same. A hyaluronate non-woven fabric manufacturing method according to the present invention includes (a) preparing hyaluronate fibers, (b) treating the hyaluronate fibers to be curled, (c) cutting the curled hyaluronate fibers, (d) forming a hyaluronate web from the curled hyaluronate fibers, and (e) needle-punching the hyaluronate web. The hyaluronate non-woven fabric of the present invention has a high hyaluronate content, is manufactured by needle punching high-strength hyaluronate fibers, and has excellent adhesion and tensile strength. Therefore, the hyaluronate non-woven fabric of the present invention can be used as a mask pack for external application on the skin, a biomaterial for tissue repairing, and an anti-adhesion coating material.

A biocompatible lyophilized pellet comprised of alginate and hyaluronate. The pellet may be used to provide a gliding surface for tissues impacted by surgery. The pellet may be delivered in a broad range of volumes to address large to micron-sized target tissues. The pellet may be hydrated before or after application. An external stimulus comprising a crosslinker may be applied to mold the pellet in situ around complex tissue geometries or to tailor the rate of bioresorption.

The invention discloses a method for preparing silk fibroin and hyaluronic acid composite gel, which comprises the following steps of: uniformly mixing hyaluronate with a first solvent to prepare hyaluronic acid gel, mixing the hyaluronic acid gel with a silk fibroin solution, shearing at a high speed, homogenizing and emulsifying, standing, shearing at a high speed, homogenizing and emulsifying, sealing and sterilizing to obtain the silk fibroin and hyaluronic acid composite gel. According to the method disclosed by the invention, reagents such as a cross-linking agent, an initiator and enzyme do not need to be additionally added; the silk fibroin solution and the hyaluronic acid gel can form uniform and mutually-crosslinked double networks in the homogeneous gel forming process, the phase separation risk is low, and the silk fibroin can generate an emulsion reaction through high-speed homogenization, so that the color of the composite gel is whiter; the preparation method disclosed by the invention is simple, rapid in emulsification and gel forming, short in production cycle, low in endotoxin of the product, high in biocompatibility, slow in degradation, small in inflammatory response and high in injection filling safety, and the composite gel can play a role in moisturizing and moisturizing after subcutaneous injection and has the whitening effects of improving skin elasticity and glossiness, fading fine wrinkles and the like.

The invention provides a preparation method of semi-fluid micro-crosslinked gel, which comprises the following steps: dissolving a mixture of sodium hyaluronates with various different molecular weights in an alkaline solution, and centrifuging after the mixture is completely dissolved, so that the sodium hyaluronates with various different molecular weights are longitudinally arranged to realize longitudinal gradient distribution; the preparation method comprises the following steps: adding a cross-linking agent dropwise in a centrifugal state to form a layered gradient cross-linked structure, further introducing ultrasonic treatment to induce transverse rearrangement of molecules, fully utilizing the limited cross-linking agent to carry out cross-linking again, improving the utilization rate of the cross-linking agent, enhancing the mechanical property of the gel and reducing the residue of the cross-linking agent, and finally carrying out post-treatment to obtain the semi-fluid micro-cross-linked gel. The semi-fluid micro-crosslinking gel prepared by the preparation method disclosed by the invention has good mechanical properties and biological integration.

A method of making a semi-synthetic scaffold is described. The method includes mixing a gelatin solution, a high molecular weight chitosan solution, and a hyaluronate solution, to form a polymeric liquid solution; pouring the polymeric liquid solution into a mold; gradually cooling the mixture to gellify and form a polymer matrix; and freezing and lyophilizing the polymer matrix to remove water and form a scaffold. Semi-synthetic scaffolds, and methods of using them for wound healing, are also described.

The application discloses a use of hyaluronic acid and its salt in taste enhancement, and belongs to the field of salt and sugar reduction. The application discloses a use of hyaluronic acid, hyaluronic acid salt and a mixture of hyaluronic acid and hyaluronic acid salt in taste enhancement, wherein the hyaluronic acid salt includes sodium salt and the like, and the molecular weight is 80.4 kDa-1090 kDa. Based on the use, the intake of condiments or taste compounds in condiments of an animal body, especially a human body, can be reduced under the condition of obtaining the same taste feeling, or a more lasting feeling of taste can be obtained under the condition of intaking the same amount of condiments or taste compounds.

The present invention relates to a cosmetic composition for topical application to the skin to control sebum overflow, comprising at least one kelp extract and at least one hydrolyzed hyaluronate.

The invention discloses an oral care composition which comprises hyaluronic acid and / or a derivative thereof and a ginseng root extract, and the weight ratio of the hyaluronic acid and / or the derivative thereof to the ginseng root extract is 50: 1 to 10000: 1; and wherein the hyaluronic acid derivative is a hyaluronate salt in the form of a salt having a cation selected from the group consisting of a sodium ion, a potassium ion, a zinc ion, an ammonium ion, mono-, di-, tri-alkanolamine, and combinations thereof.

The invention relates to a composition for promoting gene expression of skin cells in the aspects of moisturizing and repairing. The composition is composed of ceramide, composite hyaluronic acid and cosmetic acceptable auxiliary materials, ceramide is selected from ceramide 1 to ceramide 11, and the composite hyaluronic acid is a compound of hyaluronic acid and two sodium hyaluronates with different molecular weights. According to the composition of the composite hyaluronic acid and the ceramide, disclosed by the invention, the effects of moisturizing and repairing the skin can be synergistically exerted. And compared with single use of the two components, the effect is better.

To provide a method for producing hyaluronate-containing fine fibers that are excellent in moisture retention and hygroscopicity and contain high-molecular-weight hyaluronate at a high concentration, hyaluronate-containing fine fibers, and a textile product.SOLUTION: A method for producing hyaluronate-containing fine fibers comprises: a step of preparing a mixed aqueous solution containing a hyaluronate having a weight average molecular weight of 500,000 or more, a polymer having a hydrophilic group and water; and an electro-spinning of injecting the mixed aqueous solution charged from a spinning nozzle on which a high voltage is applied to spin hyaluronate-containing fine fibers. The hyaluronate-containing fine fibers contain a hyaluronate having a weight average molecular weight of 500,000 or more and a polymer having a hydrophilic group, and the hyaluronate is contained in an amount of 10 mass% or more based on a total amount of the fibers.SELECTED DRAWING: Figure 1

A biocompatible membrane comprised of alginate and hyaluronate. The membrane may be used to prevent unwanted scarring after surgery. The tissue adherence and the rate of bioresorption of the membrane may be modified through an external stimulus comprising a sequestering agent and a viscosity modifier.

The invention discloses a hydrogel sensing material for hyaluronidase activity detection and a preparation method and application thereof, and belongs to the technical field of biosensors. The hydrogel sensing material for hyaluronidase activity detection comprises: a three-dimensional network structure formed by crosslinking hyaluronate and polyethyleneimine; methylene blue molecules are loaded in the three-dimensional network structure. Based on the amount of the raw materials, the mass ratio of the hyaluronate to the methylene blue is 24-60; the mass ratio of the hyaluronate to the polyethyleneimine is 2 to 5. According to the method for quantitatively detecting hyaluronidase by using the hydrogel sensing material for hyaluronidase activity detection, the activity concentration of hyaluronidase can be rapidly and sensitively detected, the hyaluronidase activity detection can be realized only in 8-15 minutes, the specificity is good, and the anti-interference performance is strong.

This application discloses a rapidly dispersible and soluble hyaluronic acid salt and its preparation method. The method includes: granulating the hyaluronic acid salt in a fluidized bed, and spraying an aqueous ethanol solution into the granulation process to obtain rapidly dispersible and soluble hyaluronic acid or its salt. The hyaluronic acid salt prepared by this method solves the problem of hyaluronic acid salt easily agglomerating and being difficult to dissolve in water, and can be rapidly dispersed and dissolved; moreover, the obtained hyaluronic acid salt does not contain any excipients other than the raw material itself, and has a wide range of applications.

This application belongs to the field of biomedical technology, specifically relating to hyaluronic acid nanospheres, their preparation methods, and applications. This application provides a method for preparing hyaluronic acid nanospheres, which involves the interaction of nucleic acids with hyaluronic acid salts in a specific ratio. The preparation process is optimized, resulting in hyaluronic acid nanospheres with high hyaluronic acid content, strong skin permeability, and good stability, meeting the application requirements for transdermal drug delivery and skincare products. Furthermore, this application uses a non-covalent bonding method to prepare the nanospheres, simplifying the preparation process, effectively improving the gelling properties of hyaluronic acid salts, reducing stickiness at high concentrations, and broadening the application scenarios of hyaluronic acid in skincare products.

The invention relates to a preparation method of hydrogel loaded with lidocaine hydrochloride and pregabalin and the hydrogel. The preparation method comprises the following steps: mixing hyaluronic acid, dopamine hydrochloride and pure water to prepare a solution A, and then preparing a nano cellulose suspension through an ultrasonic dispersion and magnetic stirring method; the preparation method comprises the following steps: preparing a nano-cellulose suspension, mixing and stirring lidocaine hydrochloride, pregabalin, carboxymethyl chitosan and the nano-cellulose suspension to prepare a solution B, mixing the solution A with the solution B, and carrying out step-by-step oxidation crosslinking treatment to finally obtain the hydrogel loaded with lidocaine hydrochloride and pregabalin. According to the invention, the mechanical strength and adhesion strength of the hydrogel are improved, the drug loading rate, drug loading stability and release controllability are ensured, and the synergistic treatment effect of first rapid analgesia and then long-acting nerve regulation is realized.

The invention provides a zinc negative electrode composite interface layer as well as a preparation method and application thereof. The composite interface layer comprises organic salt and inorganic salt, the organic salt is selected from any one or two of alginate and hyaluronate; the inorganic salt is selected from any one or two of bicarbonate and carbonate. The composite interface layer has high mechanical performance, can prolong the protection of the interface on the zinc negative electrode while isolating water molecules, effectively reduces the possibility of decomposition or rupture of the zinc negative electrode, achieves the effect of improving the stability of the zinc negative electrode, further promotes ion transmission, improves the zinc ion deposition mode, and improves the service life of the zinc negative electrode. And the formation of by-products and zinc dendrites is greatly reduced.

The application discloses a preparation method and application of a lysine hyaluronate composition, and belongs to the technical field of cosmetics. The preparation method comprises the following steps: preparing lysine hyaluronate, preparing a HA-LYS compound, preparing a tannic acid-magnesium compound, and mixing. The HA-LYS compound is prepared in the following manner: zinc acetate is mixed with an acetic acid aqueous solution, stirring is conducted at room temperature, chitosan is added, stirring is conducted, the pH value is adjusted to 5.8-6.2, HA-LYS is added, stirring is conducted, standing is conducted, alcohol precipitation is conducted, centrifugal separation is conducted, the precipitate is taken, the precipitate is washed with an ethanol aqueous solution, drying is conducted under reduced pressure, and crushing is conducted to obtain the HA-LYS compound. The lysine hyaluronate composition prepared in the application has the characteristics of good stability, good water solubility, good skin permeability, no harmful reagent residue, and a green and simple preparation process.

To provide a contact lens packaging solution and a contact lens product.SOLUTION: A contact lens packaging solution comprises a buffer solution and 0.1 wt.%-0.4 wt.% of a moisturizing agent. The moisturizing agent comprises a salt of hyaluronic acid and a hydrolyzed hyaluronic acid derivative. The weight ratio of the hyaluronic acid salt to the hydrolyzed hyaluronic acid derivative is 1:4-4:1. The contact lens product comprises the packaging solution and a contact lens soaked in the packaging solution. The contact lens packaging solution according to the present invention can provide the contact lens wearer with sufficient initial comfort.SELECTED DRAWING: None

The invention discloses a pyrograph transfer film and a preparation method thereof, and belongs to the technical field of transfer films, the pyrograph transfer film comprises a PET release film, a printing protection layer, a printing layer and a modified adhesive layer; the printing protective layer is formed by a printing protective agent, the printing protective agent is composed of a component A and a component B according to the mass ratio of 105-223: 100, and the component A comprises hyaluronic acid, dopamine hydrochloride and nano organic zirconium; the component B comprises ionic liquid and an anti-aging agent; the modified adhesive layer is formed by a modified adhesive, and the modified adhesive is prepared by mixing styrene-butadiene latex, starch-based microgel microspheres and an ultraviolet light absorber according to the mass ratio of (80-100): (8-15): (1-3). The adhesive force between the printing protection layer and the printing layer is high, the prepared pyrograph transfer film has the advantages of being high in adhesive force, resistant to scraping, resistant to moisture, resistant to high temperature, good in weather resistance and the like, the printing layer is not prone to aging, fading, deformation, blistering, wrinkling and the like, and the transfer decoration effect of the pyrograph transfer film is guaranteed.

The invention discloses joint health-care soft sweets, and belongs to the field of health-care foods. Comprising hyaluronic acid or hyaluronate, a glucosamine compound without strongly acidic anions, a sugar body, a colloid, an additive and purified water. Compared with the prior art, firstly, glucosamine compounds, such as acetyl sugar or lemon glucosamine, which are milder in metabolism are selected as nutrient elements of the health-care soft sweets, so that the problem of stomach stimulation caused by introduction of sulfate radicals or chlorine radicals is fundamentally avoided, and damage to gastric mucosa can be reduced; secondly, the glucosamine and the hyaluronic acid are compounded according to a specific proportion, have a synergistic effect and can jointly protect articular cartilage and lubricate joints; and finally, through a specific material pretreatment method and process, the technical problems of high viscosity, moisture absorption, caking and the like caused by hyaluronic acid are solved, and the homogeneity, stability and industrial productivity of the composition in the soft sweets are ensured.

A biocompatible lyophilized pellet comprised of alginate and hyaluronate. The pellet may be used to provide a gliding surface for tissues impacted by surgery. The pellet may be delivered in a broad range of volumes to address large to micron-sized target tissues. The pellet may be hydrated before or after application. An external stimulus comprising a crosslinker may be applied to mold the pellet in situ around complex tissue geometries or to tailor the rate of bioresorption.