Immunogens used to prepare lrrc52 polyclonal antibodies

A technology of polyclonal antibodies and immunogens, applied in the direction of anti-animal/human immunoglobulins, antibodies, immunoglobulins from serum, etc., can solve the problems of contraceptive drugs that do not have male reproductive physiology

Inactive Publication Date: 2016-11-09
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, contraceptives in the current market are mainly designed for female reproductive physiology and most of them are hormonal drugs. There is no marketed contraceptive drug for male reproductive physiology.

Method used

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  • Immunogens used to prepare lrrc52 polyclonal antibodies
  • Immunogens used to prepare lrrc52 polyclonal antibodies
  • Immunogens used to prepare lrrc52 polyclonal antibodies

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Design and preparation of LRRC52 polyclonal antibody (LID1)

[0030] 1. Prepared by Beijing Xinnuo Jinke Biotechnology Co., Ltd., the antibody is named LID1, and the preparation steps are as follows:

[0031] Such as Figure 13As shown (LRRC52 N-terminal 1-244 is extracellular, 245-265 is the transmembrane region, and human C-terminal 266-313 (mouse is 266-314) is located in the cytoplasm. The 84-98 polypeptide segment marked in the figure is used as the preparation of rabbit The antigenic site of the polyclonal antibody. The arrows correspond to the specific amino acid sequence of the polypeptide in humans and mice and the sequence comparison results, showing that humans and mice are completely homologous;

[0032] Analysis and design of peptide sequences: use DNAstar software to analyze the epitope of amino acids of LRRC52, evaluate its hydrophilicity, antigenicity, surface possibility, flexible region and other indexes, comprehensively consider the amino a...

Embodiment 2

[0036] 1. The LID1 antibody was prepared as in Example 1.

[0037] 2. Experimental animals and handling

[0038] In this experiment, 14-18 weeks old C57 male mice were purchased from the Model Animal Center of Nanjing University. The mice were free to eat and drink, the room temperature was controlled at 20-25 °C, and the lighting was given according to the law of 12 hours each day and night. After the male mice were killed by cervical dislocation, the abdominal cavity was exposed, and the bilateral epididymis were taken out. The fat and mesangial tissue were separated as much as possible under the dissecting microscope, washed 2 to 3 times, the tail was cut off, placed in a petri dish filled with HS, scratched, and placed at 37 °C, 5% CO 2 Incubate in an incubator for 20 min, centrifuge at 1600 rpm for 5 min to precipitate sperm, and wash twice with HS.

[0039] 3. Experimental method

[0040] 1. Separation of human sperm

[0041] Use Percoll discontinuous gradient centr...

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Abstract

The invention belongs to the biotechnical field, and relates to an immunogen for preparing an LRRC52 polyclonal antibody. The immunogen is a polypeptide, and the amino acid sequence of the immunogen is CQNNRIREVMDYTFI. The LRRC52 polyclonal antibody prepared by using the immunogen has the advantages of high titer and good specificity. The antibody can substantially inhibit the generation of sperm potassium current after incubated with mouse or human sperms in order to substantially influence the physiologic functions of mouse and human mature sperms. The antibody can be used for researching the physiologic adjusting mechanism of the sperms as a tool medicine, and can also be used for developing novel female contraceptives as a candidate molecule.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to an immunogen for preparing LRRC52 polyclonal antibody. Background technique [0002] At present, the fertility problem has become a major global social health problem. According to the data released by the World Health Organization, the number of infertility patients in the world has reached 80 to 110 million, and it is predicted that infertility in the 21st century will become the third largest disease after tumors and cardiovascular and cerebrovascular diseases. At present, about 15% of spouses in China have fertility disorders, and 50% of the pathogenic factors are related to men. On the other hand, the current global population has reached 7 billion people, and it is still increasing at a rate of 75 million people every year. At the same time, the harm of unwanted pregnancy to human health and economy cannot be ignored. Therefore, it is of great significance to find an ideal cont...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47C07K16/18C07K16/06A61K39/395A61P15/16
CPCA61K2039/505C07K14/47C07K16/06C07K16/18
Inventor 曾旭辉郑莉萍罗韬王涛陈莹王华锋
Owner NANCHANG UNIV
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