Factory hybrid breeding method of epinephelus fuscoguttatus and epinephelus lanceolatus

Abstract

The invention relates to a factory hybrid breeding method of epinephelus fuscoguttatus and epinephelus lanceolatus, which comprises the following steps: selecting an epinephelus lanceolatus parent fish and an epinephelus fuscoguttatus parent fish for parent fish cultivation, artificially adjusting and controlling the cultivation water temperature of the epinephelus lanceolatus parent fish from 23 DEG C to 20 DEG and from 20 DEG to 30 DEG, controlling the cultivation water temperature of the epinephelus fuscoguttatus parent fish from 23 DEG C to 20 DEG and from 20 DEG to 28 DEG and taking mature sperm of the epinephelus lanceolatus parent fish in May-June of a next year for standby; and choosing a female fish of which an abdomen obviously expands, and an ovary develops to the stage IV, injecting a parturifacient, fishing an epinephelus fuscoguttatus female fish for artificial egg stripping after 36-hour effector phase, then mixing an collected epinephelus fuscoguttatus fish egg with the collected epinephelus lanceolatus mature sperm according to the volume ratio of 1000:1, adding sea water to finish fertilization, putting a fertilized egg into an incubation tank, collecting a floating eyed egg after about 20 hours and sterilizing by adopting ozone with a concentration of 0.3-0.5 ppm. Through the factory hybrid breeding method, the hybrid breeding of the epinephelus fuscoguttatus and the epinephelus lanceolatus is realized.

Description

[0001] technical field [0002] The invention relates to a hybrid breeding method of brown-spotted grouper and saddle-banded grouper. Background technique [0003] Brown Spot Grouper ( Epinephelus fuscoguttatus ), also known as brown-spotted grouper, commonly known as tiger spot and tiger head spot, is a warm-water island and reef fish. It is mainly distributed in the East China Sea and South China Sea in my country, and its resources are relatively scarce. Because of its delicious meat, fast growth and great market potential, the breeding prospect is promising. Saddle banded grouper ( Epinephelus lanceolatus ), commonly known as gentian grouper, also known as purple grouper, is a warm-water marine bottom fish and one of the largest species of grouper. It is known as the king of grouper and is mainly produced in Southeast Asia, Australian waters and the South my country Sea (Nansha Islands), waters near Taiwan. The source of seedlings of saddle grouper is scarce, and...

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a kind of industrialized hybrid breeding method of brown-spotted grouper and saddle-banded grouper. The technical problems to be solved are as follows: one is to construct Artificial maturation technology of brown-spotted grouper and saddle grouper broodstock in a factory environment to adjust the sexual maturity time of brown-spotted grouper and saddle-banded grouper, and solve the problem of brown-spotted grouper and saddle-banded grouper The development is restricted by the external environment; the second is to construct the ultra-low temperature freezing technology of brown-spotted grouper sperm, preserve the semen of saddle-banded grouper, and solve the problem of difficult fertilization due to the inconsistent sexual maturity time of brown-spotted grouper and saddle-banded grouper Problem; The third is to construct the ozone disinfection technology for fertilized eggs, carry out ozone disinfection, and effectively cut off the vertical transmission of bacteria and viral diseases from fertilized eggs to seedlings