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SSR marker site of Hyphessobrycon herbertaxelrodi and universal application of SSR marker site in native and near-source fishes

A microsatellite and heterogeneous technology, which is applied in the field of biotechnology detection, can solve the problems of rare development and application of microsatellite molecular markers, and achieve the effects of reducing experimental costs, wide application range, and low price

Active Publication Date: 2021-05-25
NANJING FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

At present, the development and application of microsatellite molecular markers for the above species is still rare.

Method used

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  • SSR marker site of Hyphessobrycon herbertaxelrodi and universal application of SSR marker site in native and near-source fishes
  • SSR marker site of Hyphessobrycon herbertaxelrodi and universal application of SSR marker site in native and near-source fishes
  • SSR marker site of Hyphessobrycon herbertaxelrodi and universal application of SSR marker site in native and near-source fishes

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Experimental program
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Embodiment 1

[0031] (1) DNA extraction of the black carp: 26 samples of the black carp were collected from Nanjing Confucius Temple Flower and Bird Market and Tianjin Baojidao Flower and Bird Market, and stored in absolute ethanol, using Nanjing Novizan Biotechnology Co., Ltd. Ltd. DNA extraction kit for DNA extraction, the specific steps are as follows:

[0032]Use clean scissors to cut up the caudal fin and transfer it to a centrifuge tube, add 230 μL Buffer GA and 20 μL PK working solution, and vortex to mix. Use a 55°C water bath until the tissue is completely enzymatically hydrolyzed. Add 250 μL Buffer GB to the digestion solution, vortex and mix at the highest speed for 20 sec, and bathe in 70°C water for 10 min. Then add 250μL of absolute ethanol to the digestion solution, and manually centrifuge for 15-20sec. Put the gDNA Columns adsorption column into the Collection Tubes 2mL collection tube. Transfer the mixed solution (including the precipitate) obtained in the previous step ...

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Abstract

The invention discloses a SSR marker site of Hyphessobrycon herbertaxelrodi and universal application of SSR marker site in native and near-source fishes, and belongs to the field of biotechnology detection. The invention provides five SSR molecular marker sites of Hyphessobrycon herbertaxelrodi, polymorphic primers of the five SSR molecular marker sites and universality of the five SSR molecular marker sites in part of species of Moenkhausia and Hyphessobrycon in Characidae, as shown in a nucleotide sequence table, amplification results of the obtained primers have high polymorphism and stability; technical data are provided for identification of genetic diversity and genetic relationship among populations of five species of Characidae Hyphessobrycon with Hyphessobrycon herbertaxelrodi as the first and Moenkhausia costae, and defects in the prior art are overcome.

Description

technical field [0001] The invention belongs to the field of biotechnology detection, and more specifically relates to a microsatellite marker site of the black different-patterned carp and its universal application between some species of the genus Carp and some species of the genus Stratocarp. Background technique [0002] Both Hyphessobrycon and Moenkhausia belong to Cypriniformes, suborder Characoidei, and Characidae in taxonomy, and most of them are native to South America. In tropical waters such as the Amazon River and the Congo River Basin in Africa, there are mostly tropical ornamental fish with bright colors and easy to raise. Among them, the smaller species are called "light fish", which were introduced into China in the middle and late last century. Popular ornamental fish. Among them, there are about 57 species in the genus Pleurotus genus, and about 30 species in the genus Rectalis. Among them, there are many well-known ornamental fish species such as the swi...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/686C12N15/11
CPCC12Q1/6888C12Q1/686C12Q2600/156C12Q2600/124C12Q2525/151
Inventor 徐润枫刘宏毅许唯
Owner NANJING FORESTRY UNIV
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