Back-portable visual in-vivo calcium imaging device

Abstract

The invention discloses a back-portable visual in-vivo calcium imaging device, and belongs to the technical field of in-vivo calcium imaging. The device includes a device body, a spine holder and fixing screws; the device body includes a track pulley system, four universal wheels and a spine holder base, the track pulley system is composed of two mutually perpendicular metal rods, wherein the twomutually perpendicular metal rods include a horizontal track and a longitudinal track, and the bottom end of the longitudinal track is provided with a microscope fixing platform; and the spine holderis connected to the spine holder base of the device main body through the four fixing screws, and is located at the inner lower part of the device main body. According to the mouse back-portable in-vivo calcium imaging device provided by the invention, a mouse spine can be fixed and is not easy to move, the mouse spine can be combined with the device main body carrying an ultramicroscope, and thedevice has the advantages of clever design, a simple and reasonable structure, stable clamping, good flexibility, convenient use, and strong compatibility, and can make calcium signal recording methods more abundant.

Description

technical field [0001] The invention relates to the technical field of in vivo calcium imaging, in particular to a back-carried visualized in vivo calcium imaging device. Background technique [0002] Calcium imaging refers to the method of using calcium ion indicator to monitor the calcium ion concentration in tissues. In the study of the nervous system, in vivo or in vitro experiments, calcium imaging technology is widely used to monitor the changes of calcium ions in hundreds or thousands of neurons at the same time, so as to detect the activity of neurons Condition. As soon as this technology appeared, it was sought after by neuroscientists all over the world, and it is still the most direct means for people to observe neuron activity. [0003] In recent years, with the development of optogenetics and in vivo calcium imaging technology, people have a very complete application method for brain in vivo calcium imaging technology. However, based on the characteristics of...

AI Technical Summary

Problems solved by technology

However, based on the characteristics of the anatomical structure and physiological functions of the spine, the application and research of the in vivo calcium imaging technology of the spinal cord is not very mature at present, because the spine itself is a movable joint with physiological curvature, and mice can easily turn when they move freely. The head bites off the imaging device fixed on the back, and because the spine keeps swinging with the movement, the imaging device will not be able to fix the focus, so that stable calcium signals cannot be taken. perfect stage

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