3 results about "Enation" patented technology

This invention belongs to the fields of biotechnology and genomics, and relates to a method and kit for capturing plant chromatin conformation. The method includes the following steps: (1) cross-linking and fixing the chromatin of a plant sample; (2) digesting the fixed chromatin using an enzyme digestion combination containing restriction endonuclease AluI and restriction endonuclease HypCh4V; (3) ligating the enzyme fragments generated in step (2) at spatially adjacent locations to form ligation products; (4) enriching the ligation products and constructing sequencing libraries using the enriched ligation products. This invention uses a combination of AluI and HypCh4V enzymes to replace traditional single-enzyme digestion, which significantly improves data resolution while reducing technical bias. It can achieve chromatin interaction mapping at a resolution of 500 bp in plants and has broad application prospects in the study of fine tissue characteristics at the single-gene level in plants.

The invention provides lentinan as well as a preparation method and application thereof, and belongs to the technical field of plant protection. According to the preparation method, a ZTC 1 + 1 II type clarifying agent is used, and step-by-step flocculation purification is carried out at 50 DEG C according to a sequence of firstly adding a component B and then adding a component A and specific stirring parameters. According to the process, impurities such as protein are efficiently removed, the active conformation of lentinan is well reserved, and the antiviral activity of the product is remarkably improved. Meanwhile, the invention finds that when the lentinan obtained by the invention is mixed with oligosaccharin.chain protein in a mass ratio of 7: 3 or 9: 1, the lentinan shows a remarkable specific synergistic effect. The invention solves the problems of poor product stability and large activity loss in the prior art, and is especially suitable for prevention and treatment of plant virus diseases such as tomato mosaic virus diseases.

The invention discloses a self-assembled nucleic acid nanoparticle as well as a preparation method and application thereof. The particle comprises (a) a three-dimensional nanostructure formed by self-folding single-stranded RNA (Ribonucleic Acid) synthesized by a T7 in-vitro transcription system; and (b) at least one functional siRNA which is loaded on the three-dimensional nanostructure and is used for targeting a key gene of a piercing-sucking mouthpart pest. The method has the beneficial effects that by simulating the three-dimensional topological structure of a natural RNA stent, the multi-arm nano-particles with efficient loading and intelligent release functions are constructed, the dynamic elastic conformation of the multi-arm nano-particles can adaptively penetrate through a plant vascular interface and an insect intestinal wall vascular barrier, and the biological distribution efficiency is remarkably improved; the modular architecture supports synchronous loading of siRNA pools targeting different physiological pathways, a'one-dose multiple-effect 'strategy of multi-gene collaborative silencing is realized, and the system is especially suitable for brown planthopper and other pests with redundant gene functions; the SARN may also modulate the release of active siRNA to overcome intermittent ingestion disorders of piercing-sucking pests.