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Dual-functional collagen scaffold material as well as preparation method and application thereof in spinal cord injury repair

A technology of collagen scaffold and spinal cord injury, which is applied in the repair of spinal cord injury. In the field of dual-function collagen scaffold materials, it can solve the problems of insufficient neuron differentiation and insufficient number of neural stem cells, so as to reduce the diffusion and promote the recovery of motor function. Effect

Active Publication Date: 2018-06-12
DUBU BIOSCIENCES
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  • Claims
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AI Technical Summary

Problems solved by technology

However, the rapid proliferation of cells and functional biomolecules can lead to insufficient numbers of neural stem cells and insufficient neuronal differentiation at the site of spinal cord injury.

Method used

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  • Dual-functional collagen scaffold material as well as preparation method and application thereof in spinal cord injury repair
  • Dual-functional collagen scaffold material as well as preparation method and application thereof in spinal cord injury repair
  • Dual-functional collagen scaffold material as well as preparation method and application thereof in spinal cord injury repair

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Embodiment Construction

[0047] In view of the deficiencies in the prior art, the inventors of the present application have prepared a bifunctional collagen scaffold material by adsorbing the CBD-EGFR-Fab antibody with collagen-specific binding ability on the nerve regeneration scaffold after long-term research and extensive practice . The bifunctional collagen scaffold material can be combined with exogenous neural stem cells to be transplanted in the transected part of the rat spinal cord. In vitro and in vivo tests prove that the bifunctional collagen scaffold material can bind exogenous neural stem cells, make them stay in the injured area, and promote their differentiation into neurons. This has a good effect on the reconstruction of neuronal circuits and the recovery of motor function after spinal cord injury, and can effectively solve the problem of the diffusion of exogenous neural stem cells from the transplantation site and the low-efficiency differentiation into neurons etc.

[0048] The ...

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Abstract

The invention discloses a dual-functional collagen scaffold material as well as a preparation method and an application thereof in spinal cord injury repair. The dual-functional collagen scaffold material consists of a nerve regeneration scaffold and a CBD-EGFR-Fab antibody which is bonded with the nerve regeneration scaffold, wherein the CBD-EGFR-Fab antibody comprises a collagen bonding area anda Fab area of an EGFR antibody which is linked to the collagen bonding area. According to the dual-functional collagen scaffold material provided by the invention, in vitro, exogenous neural stem cell bonding can be promoted and differentiation of inhibited neural stem cells towards neurons with the presence of myelin protein can be rescued, and in vivo, spread of the exogenous neural stem cellsfrom a transplantation site can be effectively relieved and a neuronal circuit can be reconstructed by promoting the differentiation of the neural stem cells towards functional neurons, so that the recovery of motor functions after spinal cord injury can be finally promoted.

Description

technical field [0001] This application specifically relates to a biological scaffold material, in particular to a bifunctional collagen scaffold material, its preparation method and its application in spinal cord injury repair. Background technique [0002] Spinal cord injuries usually cause permanent sensory and motor deficits below the level of injury. In recent decades, neural stem cell-based therapies for spinal cord injuries have undergone tremendous advancements. Neural stem cells have the potential to differentiate into neurons, astrocytes and oligodendrocytes, and can self-renew, repair and replace dead nerve cells in spinal cord injuries. At the same time, neural stem cells are undifferentiated primitive cells that do not express mature cell antigens and will not be recognized by the host immune system after transplantation. In addition, it can also fuse well with the host's nervous tissue and survive in the host for a long time. However, the few cells staying i...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/38A61L27/54
CPCA61L27/3604A61L27/3675A61L27/3834A61L27/3878A61L27/54A61L2300/256A61L2430/32
Inventor 戴建武赵燕南陈冰李晓然徐白
Owner DUBU BIOSCIENCES
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