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CTL epitope peptide of HTNV-Gn/Gc specificity and polymer and application thereof

An epitope peptide and specific technology, applied in the field of HTNV-Gn/Gc specific CTL epitope peptide, can solve the problems of non-specific prevention and treatment methods, and achieve good development and application prospects, high specificity, and high reference value Effect

Active Publication Date: 2013-12-04
FOURTH MILITARY MEDICAL UNIVERSITY
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Problems solved by technology

[0008] HFRS caused by HTNV infection has high morbidity and mortality, but so far there is no specific prevention and treatment

Method used

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  • CTL epitope peptide of HTNV-Gn/Gc specificity and polymer and application thereof
  • CTL epitope peptide of HTNV-Gn/Gc specificity and polymer and application thereof
  • CTL epitope peptide of HTNV-Gn/Gc specificity and polymer and application thereof

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Embodiment Construction

[0029] The present invention first uses the epitope prediction software SYFPEITHI and BIMAS to analyze the possible CD8 restricted by HLA-A*02 in the HTNV76-118 strain Gn / Gc sequence + The T cell 9 peptide epitope was predicted, and the predicted HTNV-Gn / Gc9 peptide was screened by the T2 cell line binding test, and HTNV-Gn / Gc CD8 with high affinity for HLA-A*02 was obtained + T cell 9-peptide epitope. The HTNV-Gn / Gc9 peptide epitope was further identified by ELISPOT assay. HLA / peptide-tetramer staining technique was used to detect the specific CTL frequency of HTNV-Gn / Gc9 peptide epitope in HLA-A*02-positive HFRS patients, and HLA-A*02-restricted HTNV-Gn / Gc The role of epitope-specific CTL responses in HFRS was analyzed. The present invention will be described in detail below, which is an explanation of the present invention rather than a limitation.

[0030] 1. Software prediction of HLA-A*0201-restricted HTNV-Gn / Gc CTL9 peptide epitope

[0031] The Gn / Gc of HTNV (strain...

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Abstract

The invention discloses CTL epitope peptide of HTNV-Gn / Gc specificity limited by HLA-A*02, as well as application thereof. The nucleotide sequence of the CTL epitope peptide is shown in SEQ ID No: 1-3. The CTL epitope peptide of HTNV-Gn / Gc specificity is epitope polypeptide limited by HLA-A*02 molecule, can induce the CD8+T cell to generate strong cell immune response, excrete IFN-gamma; the frequency of excreting IFN-gamma can be used for indicating the severity degree of an HFRS patient, i.e., the higher the frequency of excreting IFN-gamma is, the less severe the HFRS condition is; therefore, the CTL epitope peptide can be applied to the manufacturing of CTL epitope peptide vaccine, inducing of cytotoxicity T cell capable of generating CTL epitope peptide specificity, or manufacturing of HLA-A*02 / epitope peptide-compound tetramer, and has excellent development and application prospect in the field of immunological therapy of HFRS specificity.

Description

technical field [0001] The invention belongs to the technical field of prevention and treatment of Hantaan virus, and relates to HTNV-Gn / Gc specific CTL epitope peptide restricted by HLA-A*02 and application thereof. Background technique [0002] Hantaan virus (HTNV) is the prototype of the genus Hantavirus in the family Bunyaviridae, and it is also one of the important pathogenic microorganisms in the scope of verification of the protocol of the World Convention on the Prohibition of Biological Weapons. The HTNV76-118 strain first isolated by Li Haowang et al. in 1976 is the representative strain of HTNV and one of the main pathogens causing hemorrhagic fever with renal syndrome (HFRS) in my country. HFRS is a natural foci disease characterized by fever, hemorrhage and acute renal impairment, and its pathogenesis is still unclear. Among various viral infectious hemorrhagic fever diseases, HFRS is currently the most widely distributed disease with the largest number of case...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00C07K7/06C12N5/0783G01N33/68A61K39/12A61P31/14
Inventor 马樱庄然张赟张宇丝刘蓓张春梅袁斌易静李琦杨琨陈丽华宋朝君方亮金伯泉
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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