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Enzyme-labeled antibody conjugate stabilizer and application thereof

An enzyme-labeled antibody and conjugate technology, applied in the field of enzyme-labeled antibody conjugate stabilizer and biosurfactant, can solve problems such as unreported research on biosurfactants, interference with enzyme-linked immunosorbent assay results, and decreased reactivity. , to achieve the effect of large price advantage, good protection effect and extended shelf life

Active Publication Date: 2015-07-22
LIAONING CHENGDA ANIMAL PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] During the storage process of enzyme-labeled antibodies, there are problems such as easy degradation, protease catalytic activity, and decreased reactivity of antibodies and antigen-specific binding. It is often necessary to add protective agents. Commonly used protective agents include small molecule sugars, chemically synthesized surface active agents, etc., but the effect is not ideal. Although protein protective agents have a clear protective effect, they may interfere with the results of enzyme-linked immunosorbent assays, which limits their application to a certain extent.
At present, there are many examples of chemically synthesized surfactants being applied to the protease catalytic activity of enzyme-labeled antibodies and the reactivity protection of specific binding of antibodies and antigens at home and abroad, but the research on biosurfactants in this area has not been reported.

Method used

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  • Enzyme-labeled antibody conjugate stabilizer and application thereof
  • Enzyme-labeled antibody conjugate stabilizer and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Example 1: Protection of horseradish peroxidase-labeled goat anti-mouse IgG antibody.

[0031] The enzyme-labeled conjugate stabilizer includes rhamnolipid, dextran, trehalose, cysteine, and a mixture of threonine, alanine, glycine, lysine, and sodium glutamate, with a pH of 7.0~7.6 buffer salt powder and water.

[0032] The preparation method and detection method of the stabilizer solution are as follows.

[0033] 1. Preparation method.

[0034] 1.1 Prepare pH7.4 phosphate buffer solution (PBS).

[0035] 1.2 Add the above components to the buffer solution obtained in step 1.1 to prepare a stabilizer solution. The final concentration of rhamnolipid is 0.005%; the final concentration of dextran 40 is 2%; the final concentration of trehalose is 5%; the final concentration of cysteine ​​is 0.05%; threonine, alanine, glycine, lysine 1. The mixture of sodium glutamate. The final concentration of the mixture is 1.0%.

[0036] 1.3 Store the prepared solution at 4°C, take ...

Embodiment 2

[0052] Example 2: Enzyme labeling of protein A.

[0053] The enzyme-labeled conjugate stabilizer includes rhamnolipid, dextran, trehalose, cysteine, and a mixture of threonine, alanine, glycine, lysine, and sodium glutamate, with a pH of 7.0~7.6 pH buffered salt powder and water.

[0054] The preparation method and detection method of the stabilizer solution are as follows.

[0055] 1. Preparation method.

[0056] 1.1 Prepare pH7.4 phosphate buffer solution (PBS).

[0057] 1.2 Add the above components to the buffer solution obtained in step 1.1 to prepare a stabilizer solution. The final concentration of rhamnolipid is 0.002%; the final concentration of dextran 40 is 1%; the final concentration of trehalose is 5%; the final concentration of cysteine ​​is 0.05%; threonine, alanine, glycine, lysine 1. The mixture of sodium glutamate. The final concentration of the mixture is 1.0%.

[0058] 1.3 Store the prepared solution at 4°C, take it out and return to room temperature wh...

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Abstract

The invention relates to an enzyme-labeled antibody conjugate stabilizer and an application thereof. The enzyme-labeled antibody conjugate stabilizer contains rhamnolipid, dextran, mycose, cysteine, an alanine, glycine, lysine, threonine and sodium glutamate mixture, buffer salt powder with the pH value of 7.0-7.6, and water, and is used for protecting an enzyme-labeled antibody and improving the stability of the enzyme-labeled antibody in order to improve the stability and accuracy of enzyme-linked immunoassay. The enzyme-labeled antibody conjugate stabilizer prepared by adopting a biosurfactant used in the invention is novel, green and efficient, makes a enzyme-labeled antibody conjugate still have high activity in 2-8DEG C environment, obviously improves the stability of the enzyme-labeled antibody conjugate, improves the stability and accuracy of the enzyme-linked immunoassay, and has a very protection effect on other protein products.

Description

technical field [0001] The invention relates to a biosurfactant, in particular to an enzyme-labeled antibody conjugate stabilizer and application thereof, belonging to the technical field of biodetection. Specifically, after the stabilizer described in the present invention is mixed with the enzyme-labeled antibody conjugate used in ELISA, and stored at 2-8°C, the protease catalytic activity of the enzyme-labeled antibody conjugate and the protease catalytic activity of the enzyme-labeled antibody conjugate can be maintained for a long time. Antigen specific binding reactivity. Background technique [0002] Biosurfactants are metabolites with certain surface activity secreted by microorganisms during their metabolism when they are cultured under certain conditions, such as glycolipids, polysaccharide lipids, lipopeptides or neutral lipid derivatives, etc., which have While reducing surface tension, stabilizing emulsion and increasing foam, it also has the advantages of non-...

Claims

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Application Information

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IPC IPC(8): G01N33/535
CPCG01N33/535
Inventor 苏文全曾祥伟赵森汪婷于铁富袁德明卫广森
Owner LIAONING CHENGDA ANIMAL PHARMA
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