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Application of L-cysteine-enveloped nanogold in chiral recognition of tyrosine

A cysteine ​​and chiral recognition technology, applied in the field of chiral recognition, can solve the problems of large reagent loss, low sensitivity, expensive chiral chromatographic column, etc., and achieve the effect of low cost and simple method.

Inactive Publication Date: 2013-09-18
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The simplest is the optical rotation method, but the optical rotation direction of the same optically active compound will change due to the change of the measurement conditions, and there is no easy-to-distinguish relationship with the three-dimensional structure. It is easy to identify chiral compounds by measuring the optical rotation. limited
High-performance liquid chromatography, capillary electrophoresis, etc. are realized by combining separation means with photometric detection, but the chiral chromatographic columns often required are expensive, consume a lot of reagents and have high requirements for separation conditions, and the analysis time is not long enough. for quick identification
However, spectroscopic analysis and electrochemical sensors have low sensitivity and complicated identification process.

Method used

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  • Application of L-cysteine-enveloped nanogold in chiral recognition of tyrosine
  • Application of L-cysteine-enveloped nanogold in chiral recognition of tyrosine
  • Application of L-cysteine-enveloped nanogold in chiral recognition of tyrosine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The use of L-cysteine-coated gold nanoparticles in chiral recognition of tyrosine, the specific use method is as follows:

[0019] Heat and stir 50mL of chloroauric acid aqueous solution with a mass fraction of 0.04%, add 5.6mL of trisodium citrate aqueous solution with a mass fraction of 1% after boiling, the color of the solution changes from light yellow to purple, stir for 10 minutes, and the solution turns red , cooled to room temperature, and continued to stir for 13 minutes to prepare a nano-gold solution. Take 5 mL of nano-gold solution, add 100 μL of 2×10 -4 mol / L L-cysteine ​​aqueous solution was stirred at room temperature for 2 hours to prepare L-cysteine-coated gold nanoparticles. The prepared L-cysteine-coated gold nanoparticles were characterized by Tensor27 infrared spectrometer and JEM-2100 transmission electron microscope. The results are shown in figure 1 and figure 2 . Depend on figure 1 It can be seen that after L-cysteine ​​is coated with gol...

Embodiment 2

[0022] In Example 1, 120 μL of L-cysteine-coated gold nanoparticles, 300 μL of B–R buffer solution with a pH value of 4.0 were added to a 1.5 mL centrifuge tube, and then 80 μL of 2×10 -4 mol / L D-tyrosine aqueous solution, mixed evenly, the volume ratio of L-cysteine-coated nano-gold to B-R buffer solution, D-tyrosine aqueous solution was 1:2.5:0.67, static at room temperature Leave to react for 15 minutes, and the reaction solution is still red by naked eye observation. Depend on Figure 4 It can be seen that the intensity of the absorption peak of gold nanoparticles at 520nm has basically not changed, and no new absorption peaks have appeared, which is consistent with Figure 5 Most of the gold nanoparticles are still in a dispersed state, and only individual gold nanoparticles are connected to a small degree.

[0023] The preparation method of the L-cysteine-coated gold nanoparticles in this embodiment is the same as that in Example 1.

Embodiment 3

[0025] In Example 1, 120 μL of L-cysteine-coated gold nanoparticles, 240 μL of B–R buffer solution with a pH value of 4.0 were added to a 1.5 mL centrifuge tube, and then 60 μL of 1×10 -6 mol / L L-tyrosine aqueous solution, mixed evenly, the volume ratio of L-cysteine-coated gold nanoparticles to B-R buffer solution, and L-tyrosine aqueous solution was 1:2:0.5, and the volume ratio was 1:2:0.5. Leave it to react for 20 minutes, observe the color change of the reaction solution with the naked eye, and measure its absorbance A at the absorption wavelength of 520nm and 650nm by using a UV-visible spectrophotometer. After calculation, A 650 / A 520 =0.375, proved to be L-tyrosine.

[0026] The preparation method of the L-cysteine-coated gold nanoparticles in this embodiment is the same as that in Example 1.

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Abstract

The invention discloses an application of L-cysteine-enveloped nanogold in chiral recognition of tyrosine. L-tyrosine and D-tyrosine can be recognized by baked observation of color change when the concentration of the tyrosine is over 2*10<-4>mol / L; nanogold particles are gathered when the L-tyrosine is added to the L-cysteine-enveloped nanogold; the color of the solution becomes blue from red; the nanogold particles are in a disperse state when the D-tyrosine is added; the nanogold solution is still red; when the concentration of the tyrosine is 1*10-2*10<-4>mol / L, the absorbancies of the nanogold at the absorption wavelengths of 520m and 650nm are determined by an ultraviolet-visible spectrophotometer; the L-tyrosine and the D-tyrosine can be recognized; the recognition magnification can be up to 100 folds. Thus, the nanogold is simple and fast in method, and low in cost.

Description

technical field [0001] The invention belongs to the technical field of chiral recognition, and in particular relates to the chiral recognition of tyrosine enantiomers by nano-gold modified by a chiral coating reagent. Background technique [0002] Chirality is one of the basic properties of nature, and chiral recognition is the basic mode of molecular recognition in organisms. Except for the simplest glycine, all amino acids have D-type and L-type, which have different physiological activities. Therefore, chiral recognition of amino acid enantiomers is of great significance. [0003] In recent years, various chiral recognition methods have been developed. The simplest method is the optical rotation method, but the optical rotation direction of the same optically active compound will change due to the change of the measurement conditions, and there is no easy-to-distinguish relationship with the three-dimensional structure. It is easy to identify chiral compounds by measuri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/31
Inventor 李保新张琳
Owner SHAANXI NORMAL UNIV
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