Stable kit for detecting homocysteine

Abstract

The invention relates to a stable kit for detecting homocysteine, and belongs to the technical field of medical science examination and determination. The stable kit for detecting homocysteine comprises a reagent 1 and a reagent 2; the reagent 1 comprises the following components: 1-200mmol / L of an HCY reducing agent, 1.0-100mmol / L of serine, 0.5-0.8g / L of NADH, and 50-200KU / L of LDH; the reagent 2 comprises the following components: 0.1-10g / L of a stabilizing agent, 1-100KU / L of cystathionine beta-synthase, and 1-100KU / L of cystathionine beta-catabolic enzyme. The stable kit for detecting homocysteine has the advantages of convenient determination, fast detection speed, high sensitivity, high accuracy, good stability, wide linear scope and long storage time. The two reagents does not need preparation, are liquid-state reagents, and can be directly used. The kit can be stored below 4 DEG C for at least one year. Furthermore, the raw material cost is low. Clinic examination need is completely satisfied.

Description

technical field

[0001] The invention relates to a stable homocysteine ​​detection kit, which belongs to the technical field of medical examination and determination. Background technique

[0002] Homocysteine ​​is a sulfur-containing amino acid that is produced intracellularly by demethylation of methionine. Recent studies have shown that homocysteine ​​produces superoxide and peroxide damages vascular endothelial cells, changes the function of coagulation factors, increases the tendency of thrombosis, promotes atherosclerosis and thrombosis, and increases the incidence of cardiovascular diseases and Mortality increases, so measuring the concentration of homocysteine ​​in blood is of great clinical significance.

[0003] There are many methods for determining homocysteine ​​in plasma, including high performance liquid chromatography (HPLC), cycling enzyme method, fluorescence polarization immunoassay (FPIA), enzyme-linked immunoassay (EIA), amino acid analyzer determination...

Examples

Embodiment 1

[0047] The stable homocysteine ​​detection kit in this embodiment includes reagent 1 and reagent 2,

[0048] The components of reagent 1 are: TRIS: 100mmol / L, TCEP: 50mmol / L, thioglycolic acid: 10mmol / L, EDTA: 10mmol / L, serine: 100mmol / L, NADH: 0.5g / L, BSA: 5g / L , Sodium azide: 1g / L, Triton 100: 0.1%, LDH: 50KU / L;

[0049] The components of reagent 2 are: TRIS: 100mmol / L, BSA: 5g / L, soluble starch: 1g / L, glutathione: 1g / L, sodium azide: 1g / L, triton 100: 0.1 %, cystathionine β-synthase: 100KU / L, cystathionine β-decomposition enzyme: 100KU / L.

Embodiment 2

[0051] The stable homocysteine ​​detection kit in this example includes reagent 1 and reagent 2. The components of reagent 1 are: TRIS: 100mmol / L, TCEP: 80mmol / L, thioglycolic acid: 20mmol / L, EDTA: 10mmol / L L, Serine: 80mmol / L, NADH: 0.5g / L, Sucrose: 10g / L, Gentamicin Sulfate: 1g / L, Triton 100: 0.1%, LDH: 80KU / L;

[0052] The components of reagent 2 are: TRIS: 100mmol / L, sucrose: 10g / L, soluble starch: 2g / L, glutathione: 1g / L, gentamicin sulfate: 1g / L, Tween-80: 0.1%, cystathionine β-synthase: 80KU / L, cystathionine β-decomposition enzyme: 80KU / L.

Embodiment 3

[0054] The stable homocysteine ​​detection kit in this example includes reagent 1 and reagent 2. The components of reagent 1 are: phosphate buffer: 100mmol / L, TCEP: 80mmol / L, thioglycolic acid: 50mmol / L, EDTA: 10mmol / L, Serine: 100mmol / L, NADH: 0.5g / L, BSA: 5g / L, Sodium Azide: 1g / L, Triton 100: 0.1%, LDH: 100KU / L;

[0055] The components of reagent 2 are: phosphate buffer: 100mmol / L, BSA: 5g / L, soluble starch: 2g / L, glutathione: 1g / L, sodium azide: 1g / L, Triton 100 : 0.1%, cystathionine β-synthase: 50KU / L, cystathionine β-decomposition enzyme: 100KU / L.

technical field

[0001] The invention relates to a stable homocysteine ​​detection kit, which belongs to the technical field of medical examination and determination. Background technique

[0002] Homocysteine ​​is a sulfur-containing amino acid that is produced intracellularly by demethylation of methionine. Recent studies have shown that homocysteine ​​produces superoxide and peroxide damages vascular endothelial cells, changes the function of coagulation factors, increases the tendency of thrombosis, promotes atherosclerosis and thrombosis, and increases the incidence of cardiovascular diseases and Mortality increases, so measuring the concentration of homocysteine ​​in blood is of great clinical significance.

[0003] There are many methods for determining homocysteine ​​in plasma, including high performance liquid chromatography (HPLC), cycling enzyme method, fluorescence polarization immunoassay (FPIA), enzyme-linked immunoassay (EIA), amino acid analyzer determination...

Examples

Embodiment 1

[0047] The stable homocysteine ​​detection kit in this embodiment includes reagent 1 and reagent 2,

[0048] The components of reagent 1 are: TRIS: 100mmol / L, TCEP: 50mmol / L, thioglycolic acid: 10mmol / L, EDTA: 10mmol / L, serine: 100mmol / L, NADH: 0.5g / L, BSA: 5g / L , Sodium azide: 1g / L, Triton 100: 0.1%, LDH: 50KU / L;

[0049] The components of reagent 2 are: TRIS: 100mmol / L, BSA: 5g / L, soluble starch: 1g / L, glutathione: 1g / L, sodium azide: 1g / L, triton 100: 0.1 %, cystathionine β-synthase: 100KU / L, cystathionine β-decomposition enzyme: 100KU / L.

Embodiment 2

[0051] The stable homocysteine ​​detection kit in this example includes reagent 1 and reagent 2. The components of reagent 1 are: TRIS: 100mmol / L, TCEP: 80mmol / L, thioglycolic acid: 20mmol / L, EDTA: 10mmol / L L, Serine: 80mmol / L, NADH: 0.5g / L, Sucrose: 10g / L, Gentamicin Sulfate: 1g / L, Triton 100: 0.1%, LDH: 80KU / L;

[0052] The components of reagent 2 are: TRIS: 100mmol / L, sucrose: 10g / L, soluble starch: 2g / L, glutathione: 1g / L, gentamicin sulfate: 1g / L, Tween-80: 0.1%, cystathionine β-synthase: 80KU / L, cystathionine β-decomposition enzyme: 80KU / L.

Embodiment 3

[0054] The stable homocysteine ​​detection kit in this example includes reagent 1 and reagent 2. The components of reagent 1 are: phosphate buffer: 100mmol / L, TCEP: 80mmol / L, thioglycolic acid: 50mmol / L, EDTA: 10mmol / L, Serine: 100mmol / L, NADH: 0.5g / L, BSA: 5g / L, Sodium Azide: 1g / L, Triton 100: 0.1%, LDH: 100KU / L;

[0055] The components of reagent 2 are: phosphate buffer: 100mmol / L, BSA: 5g / L, soluble starch: 2g / L, glutathione: 1g / L, sodium azide: 1g / L, Triton 100 : 0.1%, cystathionine β-synthase: 50KU / L, cystathionine β-decomposition enzyme: 100KU / L.

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