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Stable kit for detecting homocysteine

A homocysteine ​​and detection kit technology, applied in the field of medical testing and determination, can solve the problems of large variation of test data, long test time, complicated operation, etc., and achieve the effect of fast detection speed, convenient measurement and long storage time

Active Publication Date: 2015-12-23
NINGBO RUI BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Similarly, ion chromatography and capillary electrophoresis are mainly used in experimental research, and are rarely used in clinical practice, and capillary electrophoresis is similar to high performance liquid chromatography, but there are still complex operations, long test time and test data Disadvantages such as relatively large variation
[0008] Enzyme-linked immunoassay (EIA): This method is a common method for clinical determination of homocysteine. It has the advantages of simple and fast operation, good repeatability and good stability. However, most of this method requires manual operation and is time-consuming. Long and expensive reagents

Method used

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  • Stable kit for detecting homocysteine
  • Stable kit for detecting homocysteine
  • Stable kit for detecting homocysteine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The stable homocysteine ​​detection kit in this embodiment includes reagent 1 and reagent 2,

[0048] The components of reagent 1 are: TRIS: 100mmol / L, TCEP: 50mmol / L, thioglycolic acid: 10mmol / L, EDTA: 10mmol / L, serine: 100mmol / L, NADH: 0.5g / L, BSA: 5g / L , Sodium azide: 1g / L, Triton 100: 0.1%, LDH: 50KU / L;

[0049] The components of reagent 2 are: TRIS: 100mmol / L, BSA: 5g / L, soluble starch: 1g / L, glutathione: 1g / L, sodium azide: 1g / L, triton 100: 0.1 %, cystathionine β-synthase: 100KU / L, cystathionine β-decomposition enzyme: 100KU / L.

Embodiment 2

[0051] The stable homocysteine ​​detection kit in this example includes reagent 1 and reagent 2. The components of reagent 1 are: TRIS: 100mmol / L, TCEP: 80mmol / L, thioglycolic acid: 20mmol / L, EDTA: 10mmol / L L, Serine: 80mmol / L, NADH: 0.5g / L, Sucrose: 10g / L, Gentamicin Sulfate: 1g / L, Triton 100: 0.1%, LDH: 80KU / L;

[0052] The components of reagent 2 are: TRIS: 100mmol / L, sucrose: 10g / L, soluble starch: 2g / L, glutathione: 1g / L, gentamicin sulfate: 1g / L, Tween-80: 0.1%, cystathionine β-synthase: 80KU / L, cystathionine β-decomposition enzyme: 80KU / L.

Embodiment 3

[0054] The stable homocysteine ​​detection kit in this example includes reagent 1 and reagent 2. The components of reagent 1 are: phosphate buffer: 100mmol / L, TCEP: 80mmol / L, thioglycolic acid: 50mmol / L, EDTA: 10mmol / L, Serine: 100mmol / L, NADH: 0.5g / L, BSA: 5g / L, Sodium Azide: 1g / L, Triton 100: 0.1%, LDH: 100KU / L;

[0055] The components of reagent 2 are: phosphate buffer: 100mmol / L, BSA: 5g / L, soluble starch: 2g / L, glutathione: 1g / L, sodium azide: 1g / L, Triton 100 : 0.1%, cystathionine β-synthase: 50KU / L, cystathionine β-decomposition enzyme: 100KU / L.

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Abstract

The invention relates to a stable kit for detecting homocysteine, and belongs to the technical field of medical science examination and determination. The stable kit for detecting homocysteine comprises a reagent 1 and a reagent 2; the reagent 1 comprises the following components: 1-200mmol / L of an HCY reducing agent, 1.0-100mmol / L of serine, 0.5-0.8g / L of NADH, and 50-200KU / L of LDH; the reagent 2 comprises the following components: 0.1-10g / L of a stabilizing agent, 1-100KU / L of cystathionine beta-synthase, and 1-100KU / L of cystathionine beta-catabolic enzyme. The stable kit for detecting homocysteine has the advantages of convenient determination, fast detection speed, high sensitivity, high accuracy, good stability, wide linear scope and long storage time. The two reagents does not need preparation, are liquid-state reagents, and can be directly used. The kit can be stored below 4 DEG C for at least one year. Furthermore, the raw material cost is low. Clinic examination need is completely satisfied.

Description

technical field [0001] The invention relates to a stable homocysteine ​​detection kit, which belongs to the technical field of medical examination and determination. Background technique [0002] Homocysteine ​​is a sulfur-containing amino acid that is produced intracellularly by demethylation of methionine. Recent studies have shown that homocysteine ​​produces superoxide and peroxide damages vascular endothelial cells, changes the function of coagulation factors, increases the tendency of thrombosis, promotes atherosclerosis and thrombosis, and increases the incidence of cardiovascular diseases and Mortality increases, so measuring the concentration of homocysteine ​​in blood is of great clinical significance. [0003] There are many methods for determining homocysteine ​​in plasma, including high performance liquid chromatography (HPLC), cycling enzyme method, fluorescence polarization immunoassay (FPIA), enzyme-linked immunoassay (EIA), amino acid analyzer determination...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 谢蒙张闻周海滨王建飞
Owner NINGBO RUI BIO TECH
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