Molecular design phycoerythrocyanin beta subunit fluorescent protein combining phycocyanobilin (PCB) and application thereof

A phycoerythrin and phycocyanin technology, which is applied to a molecularly designed phycoerythrin β subunit-like fluorescent protein combined with phycocyanin PCB and its application field, can solve complex technical procedures and high background. , the difficulty of quantitative determination by fluorescence immunoassay technology, etc., to achieve the effect of high fluorescence efficiency, easy purification, and good sensitivity
CN101759795AInactive Publication Date: 2010-06-30GUANGZHOU TEBSUN BIO TECH DEV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GUANGZHOU TEBSUN BIO TECH DEV
Publication Date
2010-06-30
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a molecular design phycoerythrocyanin beta subunit fluorescent protein combining phycocyanobilin (PCB), fusion protein formed by the phycoerythrocyanin beta subunit fluorescent protein and streptavidin, and mutant thereof, having the sequences 1, 2, 3 and 4. The invention also discloses a method for directly using the fluorescent protein fused with the streptavidin for fluorescence immunoassay; and due to thioether bond, 153th cysteine residue conserved by phycoerythrocyanin beta subunit can be combined with the phycocyanobilin (PCB), and is prepared into beta subunit of fluorescent phycoerythrocyanin by escherichia coli of genetic engineering. The spectrum property of the phycoerythrocyanin is completely different from that of the natural phycoerythrocyanin, and is convenient for purification due to being provided with His-tag label. Furthermore, the dissolubility can be improved, the fusion protein can be formed by the phycoerythrocyanin beta subunit fluorescent protein and the streptavidin, the fluorescent protein can be directly used for fluorescence immunoassay, and the invention is beneficial to the application in various fields.
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Description

technical field

[0001] The present invention relates to a molecularly designed phycoerythrin beta subunit fluorescent protein combined with phycocyanin PCB and its application, belonging to the field of pigment protein materials in biotechnology, in particular to molecularly designed phycocyanin combined The phycoerythrin beta subunit fluorescent protein of PCB, the fusion protein formed with streptavidin and its mutant, and the detection method for detecting soluble antigen or antibody by using the fusion protein. Background technique

[0002] Phycobiliproteins are functional components of photosynthetic light-harvesting complexes in cyanobacteria and red algae. According to their absorption spectrum and fluorescence spectrum characteristics, phycobiliproteins can be divided into phycoerythrin (CPE for short), phycoerythrocyanin (PEC for short), phycocyanin (CPC for short) and variable phycocyanin. (allophycocyanin, referred to as APC). CPE, PEC, CPC, and APC contain alph...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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