Allophycocyanin subunits fluorescent protein combined with phycocyanobilin PCB and application thereof

A technology for allophycocyanin and phycocyanin, which is used in the detection field of soluble antigen or antibody detection, and can solve the problems of high background, non-specific staining problems that have not been completely solved, and difficulties in quantitative determination by fluorescent immunological technology.
CN101759796AInactive Publication Date: 2010-06-30GUANGZHOU TEBSUN BIO TECH DEV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GUANGZHOU TEBSUN BIO TECH DEV
Publication Date
2010-06-30
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to allophycocyanin subunits fluorescent protein combined with phycocyanobilin PCB and fusion protein formed by allophycocyanin subunits fluorescent protein and streptavidin, the sequences are from sequence 1 to sequence 10; furthermore, the invention discloses a method of the fluorescent protein fused with streptavidin for fluorescent immunological detection directly; allophycocyanin subunit conserved cysteine residue is combined with the phycocyanobilin PCB by a thioether bond, and the subunit of the fluorescent allophycocyanin can be obtained by utilizing escherichia coli through genetic engineering, the spectroscopy of the protein carries His-tag label, therefore, purification is not only convenient, but also the dissolubility of the protein can be improved; the protein is combined with the streptavidin to form the fusion protein for fluorescent immunological detection directly, thereby being beneficial to the application of the protein in all kinds of the field.
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Description

technical field

[0001] The invention relates to an allophycocyanin subunit fluorescent protein combined with phycocyanin PCB, which belongs to the field of pigment protein materials in biotechnology, in particular to an allophycocyanin subunit fluorescent protein combined with phycocyanin PCB, The fusion protein formed with streptavidin and its mutant, and the detection method for detecting soluble antigen or antibody by using the fusion protein. Background technique

[0002] Phycobiliproteins are functional components of photosynthetic light-harvesting complexes in cyanobacteria and red algae. According to their absorption spectrum and fluorescence spectrum characteristics, phycobiliproteins can be divided into phycoerythrin (CPE for short), phycoerythrocyanin (PEC for short), phycocyanin (CPC for short) and variable phycocyanin. (allophycocyanin, referred to as APC). CPE, PEC, CPC, and APC contain alpha and beta subunits, and in each subunit, phycobilin (phycobilin) ​​is...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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