Construction method of novel photo-assisted bipolar self-energized aptamer sensing device

An aptamer sensor and construction method technology, applied in the field of electrochemical biosensing, can solve problems such as threats to human health, labor-intensive, and complex operations, and achieve excellent power output performance, improved utilization, and good energy level matching. Effect

Active Publication Date: 2021-03-09
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the residue problem in animal-derived food caused by excessive use of SMZ is a serious threat to human health
Currently, methods for detecting SMZ include enzyme-linked immunoassay, high-performance liq

Method used

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  • Construction method of novel photo-assisted bipolar self-energized aptamer sensing device
  • Construction method of novel photo-assisted bipolar self-energized aptamer sensing device
  • Construction method of novel photo-assisted bipolar self-energized aptamer sensing device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] (1) B-TiO 2 preparation of

[0051] Measure 1.7mL of tetrabutyl titanate and 2.5mL of ethanol and mix them evenly to obtain solution A; measure and mix 0.1mL of concentrated nitric acid, 2.5mL of ethanol and 0.5mL of water to obtain solution B; Add dropwise into solution B, stir for 0.5h to obtain mixed solution C, transfer it to a stainless steel autoclave, and react at 180°C for 12h to obtain solid product titanium dioxide; weigh 200mg of titanium dioxide and 66.66mg of sodium borohydride and mix them in a mortar Grind well, transfer to a porcelain crucible, put into a tube furnace, and calcinate at 350°C for 1h in an argon atmosphere with a heating rate of 10°C / min to obtain B-TiO 2 nanoparticles.

[0052] (2)Cu 2 Preparation of O / 3DNGH

[0053] First, 50 mL of graphene oxide dispersion (1 g / mL) was stirred with 2 g of urea, transferred to an autoclave, and reacted at 180 ° C for 12 h to obtain 3DNGH. Then, dissolve 0.25g of copper nitrate in 50mL of water, drop...

Embodiment 2

[0067] (1) B-TiO 2 Preparation of nanoparticles

[0068] Measure 1mL of tetrabutyl titanate and 1.5mL of ethanol and mix them evenly to obtain solution A; measure and mix 0.05mL of concentrated nitric acid, 1.25mL of ethanol and 0.25mL of water to obtain solution B; Add to solution B, stir for 0.5h to obtain mixed solution C, transfer to a stainless steel autoclave, react at 180°C for 10h, and obtain solid product titanium dioxide; weigh 100mg of titanium dioxide and mix with 33.33mg of sodium borohydride, grind in a mortar fully, transferred to a porcelain crucible, placed in a tube furnace, and calcined at 300°C for 1h in an argon atmosphere with a heating rate of 10°C / min to obtain B-TiO 2 nanoparticles.

[0069] (2)Cu 2 Preparation of O / 3DNGH

[0070] First, 50 mL of graphene oxide dispersion (1 g / mL) was stirred with 2 g of urea, transferred to an autoclave, and reacted at 180 ° C for 12 h to obtain 3DNGH. Then, dissolve 0.25g of copper nitrate in 50mL of water, drop...

Embodiment 3

[0073] (1) B-TiO 2 Preparation of nanoparticles

[0074] Measure 3mL of tetrabutyl titanate and 4mL of ethanol and mix them evenly to obtain solution A; measure and mix 0.15mL of concentrated nitric acid, 3.75mL of ethanol and 0.75mL of water to obtain solution B; add solution A dropwise into solution B, stirred for 0.5h to obtain mixed solution C, transferred to a stainless steel autoclave, and reacted at 180°C for 14h to obtain a solid product of titanium dioxide; weigh 300mg of titanium dioxide and 100mg of sodium borohydride, mix them in a mortar and grind them thoroughly, Transfer to a porcelain crucible, put it into a tube furnace, and calcinate at 400 °C for 1 h in an argon atmosphere with a heating rate of 10 °C / min to obtain B-TiO 2 nanoparticles.

[0075] (2)Cu 2 Preparation of O / 3DNGH

[0076] First, 50 mL of graphene oxide dispersion (1 g / mL) was stirred with 2 g of urea, transferred to an autoclave, and reacted at 180 ° C for 12 h to obtain 3DNGH. Then, disso...

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Abstract

The invention provides a construction method of a novel photo-assisted bipolar self-energized aptamer sensor, which uses a universal meter as a direct reading strategy, and comprises the following steps: step 1, preparing a photo-anode material black titanium dioxide (BTiO2) and a photo-cathode material three-dimensional nitrogen-doped graphene hydrogel loaded cuprous oxide nanosphere (Cu2O/3DNGH); and step 2, constructing the photo-assisted bipolar self-energized aptamer sensor for detecting sulfamethazine. The novel photo-assisted bipolar self-energized aptamer sensor constructed by the invention does not need an external power supply, the detection device supplies energy to the detection process of the sensor, and a simple universal meter is adopted as a direct reading strategy, so thatminiaturization and portability are facilitated, and field detection is realized. Meanwhile, both the anode and the cathode of the sensor are made of semiconductor materials, so that the use of bioactive components and precious metal electrodes Pt is avoided, the solar energy utilization efficiency is greatly improved, and the manufacturing cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of electrochemical biosensing, and relates to a construction method of a self-powered aptamer sensor device based on a light-assisted bipolar fuel cell. Background technique [0002] Self-powered electrochemical sensor is an emerging electrochemical detection technology. Unlike traditional electrochemical sensing systems, self-powered sensors do not require an external power supply and can supply energy for their own sensing process. By changing the concentration of the target Converted to changes in power signals (such as open circuit voltage, current density or power, etc.), to achieve quantitative detection of targets. Self-powered electrochemical sensing technology has unique advantages in promoting sensor miniaturization, convenience, and low cost. For example, no external power supply is required, and only two electrodes (anode and cathode) are used to realize electrochemical detection, which is conduc...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N33/53
CPCG01N27/305G01N33/9446Y02E60/50
Inventor 王坤张萌张真真郝楠戴震
Owner JIANGSU UNIV
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