Fluorescence liquid drop sorting system based on digital microfluidics and sorting method thereof

Abstract

The invention discloses a fluorescence liquid drop sorting system based on digital microfluidics and a sorting method thereof. The fluorescence liquid drop sorting system comprises a digital microfluidic chip, an integrated circuit and a fluorescence excitation and acquisition module. The digital microfluidic chip is connected with the integrated circuit; and the fluorescence excitation and acquisition module is connected with the digital microfluidic chip and the integrated circuit respectively. According to the fluorescence liquid drop sorting system based on the digital microfluidics, disclosed by the invention, generation, transportation and sorting processes of liquid drops are carried out based on an on-chip dielectric wetting principle, so that a realization mechanism of the third party does not need to be added and the miniaturization of the system is relatively easy to realize; and single liquid drops are manipulated and analyzed in the processes, and a biochemical staining and fluorescence protein marking technology is combined, so that the fluorescence liquid drop sorting system can be used for detecting and sorting single cells, secreted proteins or microorganisms, and is used for the fields of early-stage disease diagnosis and treatment and the like.

Description

technical field [0001] The invention belongs to the technical field of microfluidics, and in particular relates to a fluorescent droplet sorting system and a sorting method based on digital microfluidics. Background technique [0002] In the existing droplet sorting methods, the sorting control module will act on the area where the droplets flow instead of the individual droplets themselves, so there will be inaccurate sorting, missing or wrong selection. [0003] Secondly, the continuous microfluidic technology used in the commonly used fluorescent droplet sorting system is realized by glass or plastic-based microfluidic channels, which is suitable for some simple pre-defined applications, and it is difficult to achieve complex processing. And generally can only work in the serial mode, the work efficiency is low. Since the working parameters (such as pressure, fluid resistance, electric field strength, etc.) are different throughout the microfluidic system, microfluidics ...

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Problems solved by technology

[0005] In addition, in the commonly used fluorescent droplet sorting device based on microfluidics, the method of detecting sample particles using excited fluorescence signals has been widely used. For example, Zhenning Cao et al. proposed Droplet sorting based on the number of encapsulated particles using a solenoid valve However, after the detection of sample particles, screening mechanisms have been developed such as: optical tweezers, mechanical switches, water conductivity, dielectrophoresis, etc., which have expensive technical means, require micro-processing channels, and need to add third-party sorting modules, etc. short board

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