On-site rapid detection method for volatile benzaldehyde in expired gas of human body

Abstract

The invention relates to an on-site rapid detection method for volatile benzaldehyde in expired gas of a human body. The on-site rapid detection method comprises the following steps of S1, preparing gold nanospheres modified by p-mercaptoaniline and carbon quantum dots modified by mercaptosuccinic acid; S2, adopting electrostatic interaction and a layer-by-layer assembly strategy to prepare a GNPs-CQDs assembly body and a GNPs-CQDs@MOFs core-shell nano material; S3, the GNPs-CQDs@MOFs core-shell nanometer material being deposited on a paper base, and a GNPs-CQDs@MOFs paper chip being obtained; S4, gas benzaldehyde in exhaled gas being extracted through the GNPs-CQDs@MOFs paper chip; and S5, detecting benzaldehyde by adopting a portable Raman spectrometer and an ultraviolet lamp in a surface enhanced Raman spectroscopy (SERS) and fluorescent double-probe mode. Compared with the prior art, the method has advantages of high analysis speed, high sensitivity, low sample consumption, wide application range, simplicity and convenience in operation and the like.

Description

technical field

[0001] The invention relates to the technical field of on-site detection, in particular to an on-site rapid detection method for volatile benzaldehyde in human exhaled air. Background technique

[0002] Lung cancer ranks first in the incidence of malignant tumors in the world. In many large and medium-sized cities in my country, its incidence and mortality have leapt to the top of common tumors, seriously endangering human health. About 10,000 people die of lung cancer every year in our country, and among all stages of lung cancer, the annual survival rate of advanced lung cancer is extremely low. With the continuous development of medical science and technology, the level of diagnosis and treatment of lung cancer has been significantly improved, but it is still far from meeting the needs of human and clinical work. A large number of studies have shown that the most effective way to reduce the mortality rate of lung cancer is its early diagnosis and treatmen...

Examples

Embodiment 1

[0046] (1) Gold nanospheres modified with p-mercaptoaniline

[0047] Add 100mL ultra-purified water and 1.0mL 10% chloroauric acid (HAuCl 4 ) solution and heated to boiling. Immediately afterward, 1.0 mL of 1% sodium citrate solution was added to synthesize gold nanoparticles with an average diameter of 50 nm. When the color of the solution changed from colorless to wine red, GNPs were formed, and 1.0 mL of 1% polyvinylpyrrolidone (PVP) solution was added, and the heating was stopped. After cooling to room temperature, filter with a 0.45 μM filter membrane, and finally store the prepared gold nanosphere solution (GNPs) at 4°C.

[0048] Add 0.5 mL of 1 mM p-mercaptoaniline to 5 mL of the purified gold nanosphere solution. The mixture was equilibrated at 30°C for 24 hours, centrifuged at 8000 rpm for 5 minutes to remove excess p-mercaptoaniline in the mixture, and ultrasonicated for 30 minutes to make the solution fully distributed evenly.

[0049] (2) Carbon quantum dots mo...

Embodiment 2

[0063] Detection of Volatile Benzaldehyde in Human Exhaled Breath

[0064] Figure 8 Schematically provides the flow chart of benzaldehyde in the exhaled breath of the embodiment of the present invention, described detection method comprises the following steps:

[0065] (1) Prepare gold nanospheres modified with p-mercaptoaniline, the steps are the same as in Example 1;

[0066] (2) Prepare carbon quantum dots modified with mercaptosuccinic acid, the steps are the same as in Example 1;

[0067] (3) Prepare GNPs-CQDs@MOFs core-shell nanomaterials, the steps are the same as in Example 1;

[0068] (4) Construct GNPs-CQDs@MOFs paper chip and VG-PBM device, the steps are the same as in Example 1;

[0069] (5) Fluorescent detection of volatile benzaldehyde in exhaled air.

[0070] By recruiting 10 volunteers, each participant was asked to collect breath samples using a nitrogen-purged 500-mL Tedlar bag with an attached mouthpiece after obtaining informed consent from the voluntee...

technical field

[0001] The invention relates to the technical field of on-site detection, in particular to an on-site rapid detection method for volatile benzaldehyde in human exhaled air. Background technique

[0002] Lung cancer ranks first in the incidence of malignant tumors in the world. In many large and medium-sized cities in my country, its incidence and mortality have leapt to the top of common tumors, seriously endangering human health. About 10,000 people die of lung cancer every year in our country, and among all stages of lung cancer, the annual survival rate of advanced lung cancer is extremely low. With the continuous development of medical science and technology, the level of diagnosis and treatment of lung cancer has been significantly improved, but it is still far from meeting the needs of human and clinical work. A large number of studies have shown that the most effective way to reduce the mortality rate of lung cancer is its early diagnosis and treatmen...

Examples

Embodiment 1

[0046] (1) Gold nanospheres modified with p-mercaptoaniline

[0047] Add 100mL ultra-purified water and 1.0mL 10% chloroauric acid (HAuCl 4 ) solution and heated to boiling. Immediately afterward, 1.0 mL of 1% sodium citrate solution was added to synthesize gold nanoparticles with an average diameter of 50 nm. When the color of the solution changed from colorless to wine red, GNPs were formed, and 1.0 mL of 1% polyvinylpyrrolidone (PVP) solution was added, and the heating was stopped. After cooling to room temperature, filter with a 0.45 μM filter membrane, and finally store the prepared gold nanosphere solution (GNPs) at 4°C.

[0048] Add 0.5 mL of 1 mM p-mercaptoaniline to 5 mL of the purified gold nanosphere solution. The mixture was equilibrated at 30°C for 24 hours, centrifuged at 8000 rpm for 5 minutes to remove excess p-mercaptoaniline in the mixture, and ultrasonicated for 30 minutes to make the solution fully distributed evenly.

[0049] (2) Carbon quantum dots mo...

Embodiment 2

[0063] Detection of Volatile Benzaldehyde in Human Exhaled Breath

[0064] Figure 8 Schematically provides the flow chart of benzaldehyde in the exhaled breath of the embodiment of the present invention, described detection method comprises the following steps:

[0065] (1) Prepare gold nanospheres modified with p-mercaptoaniline, the steps are the same as in Example 1;

[0066] (2) Prepare carbon quantum dots modified with mercaptosuccinic acid, the steps are the same as in Example 1;

[0067] (3) Prepare GNPs-CQDs@MOFs core-shell nanomaterials, the steps are the same as in Example 1;

[0068] (4) Construct GNPs-CQDs@MOFs paper chip and VG-PBM device, the steps are the same as in Example 1;

[0069] (5) Fluorescent detection of volatile benzaldehyde in exhaled air.

[0070] By recruiting 10 volunteers, each participant was asked to collect breath samples using a nitrogen-purged 500-mL Tedlar bag with an attached mouthpiece after obtaining informed consent from the voluntee...

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